ELISA Incubation time? - (Oct/23/2006 )
What will be the consequence if the primary and secondary antibody of captured ELISA are incubated for 2 days in 4 degree C separately? Will they denature?
Also, for the process of blocking? What is the difference between using non-fat dry milk and BSA?
Thank you for your attention!!
-Gambler-
QUOTE (Gambler @ Oct 23 2006, 11:20 AM)
What will be the consequence if the primary and secondary antibody of captured ELISA are incubated for 2 days in 4 degree C separately? Will they denature?
Also, for the process of blocking? What is the difference between using non-fat dry milk and BSA?
Thank you for your attention!!
Also, for the process of blocking? What is the difference between using non-fat dry milk and BSA?
Thank you for your attention!!
Q1: Ab should not be denaturized
Q2: milk contains various proteins but predominantly casein; BSA is bovine serum albumin; if there is no existing or well working protocol, one has to test which blocking solution will be the best; depends very much on Ab and antigens
-The Bearer-
For ELISA, most people use BSA.
-Minnie Mouse-