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ELISA background - (Jun/01/2006 )

Hello, what are the reasons for having high background in ELISA ? My highest absorbance was 1.79 and my control absorbance was 0.19, does it consider a " high" background? thanks !

-enolase-

QUOTE (enolase @ Jun 1 2006, 12:30 PM)
Hello, what are the reasons for having high background in ELISA ? My highest absorbance was 1.79 and my control absorbance was 0.19, does it consider a " high" background? thanks !


No.
0-0.2 low background
0.2-0.4 medium background
>0.5 high background

-Minnie Mouse-

0.19? I wish!!!

-swanny-