ELISA - Capturing and detecting Abs concentration - (Nov/24/2008 )

What would be the ideal concentration to use for capturing and detecting antibodies? From my understanding, capturing Ab would be at least 1ug/ml and usually much more higher than detecting Ab, which is usually prepared in much lower concentration. Please advice, thanks...

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The ideal concentration must be determend for the specific test by checkerboardtitrations.
Capturing with 1- 10 ug/ml is a good starting point but for the detecting antibody it is dependend form the quality of the antibody en the detectionsystem used Enzym,Fluormetric etc. from 1:1000 up to 1:100,000 and maybe higher

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