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Having trouble with stripping PVDF membrane during WesterDot blot - (Jan/22/2011 )

Hello, I am wondering if anyone has faced problems with stripping PVDF membranes after using a UV transilluminator imaging system. I use a commercially available stripping solution for 20min but i couldn't remove the stain at all. Do you have any idea about what the problem is?
Thank you in advance

-Niki Argentou-

Hi, please give us some more details.

Exactly what chromogenic system are you using?
Has the membrane been dry at the meantime?
How long do you leave it under the illuminator?