what do you recommend for Semi-Dry unit - (Aug/12/2011 )
our lab have been using wet transfer for our protein and we are thinking to switch to semi-dry. Does anyone know whats the best company on the market who sells these units. And do you guys suggest using the semi-dry for all of our transfer for every protein (large and small, phosphorlated or unphosphorlated..etc)
BioRad has a nice system.
Hi! We have the Trans-Blot SD Semi-Dry Transfer Cell from Bio Rad and use it frequently for transfer of phospho-, non-phospho- ubiquitinated-, sumoylated-, large and small proteins.
Of course if your protein is heat labile, then I would recommend using the wet system.