Trouble with the GFP signal - (Jan/06/2012 )

I'm generating a cell line stably expressing a GFP fusion protein. I have one clone that is fluorescent under the microscope. However, I cannot detect the GFP fusion signal by western blot with anti-GFP antibody =( The antibody is fine as indicated by the positive control. Is fluorescence microscopy is more sensitive than western blot?

Thanks!
Athena

possibly, or it could be tha you are seeing auto-fluorescence of the cells rather than GFP.