Trouble with the GFP signal - (Jan/06/2012 )
I'm generating a cell line stably expressing a GFP fusion protein. I have one clone that is fluorescent under the microscope. However, I cannot detect the GFP fusion signal by western blot with anti-GFP antibody =( The antibody is fine as indicated by the positive control. Is fluorescence microscopy is more sensitive than western blot?
possibly, or it could be tha you are seeing auto-fluorescence of the cells rather than GFP.