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Use of wrong TBS concentration - (Jun/01/2012 )

While running a WB, I used a mislabeled container of TBS. Instead of using the usual 1XTBS, I used the 10XTBS for washing and overnight soaking. Would anyone be able to tell me how detrimental this may (or hopefully may not) be to my results?



It may wash out secondary antibodies attached to primary antibody.

-Arun Kumaran-