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Use of wrong TBS concentration - (Jun/01/2012 )

While running a WB, I used a mislabeled container of TBS. Instead of using the usual 1XTBS, I used the 10XTBS for washing and overnight soaking. Would anyone be able to tell me how detrimental this may (or hopefully may not) be to my results?

Thanks!

-researchgal-

It may wash out secondary antibodies attached to primary antibody.

-Arun Kumaran-