Use of wrong TBS concentration - (Jun/01/2012 )
While running a WB, I used a mislabeled container of TBS. Instead of using the usual 1XTBS, I used the 10XTBS for washing and overnight soaking. Would anyone be able to tell me how detrimental this may (or hopefully may not) be to my results?
Thanks!
-researchgal-
It may wash out secondary antibodies attached to primary antibody.
-Arun Kumaran-