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Top : New Forum Archives (2009-): : Protein-and-Proteomics
31. Use of speedvac to dry in-gel peptide digest before sending for LCMS - (reply: 2)
32. doublets on 2D gel - (reply: 4)
33. RNA later and 2D SDS PAGE - (reply: 3)
34. Peptide quantification - (reply: 4)
35. Affinity purification glycine elution chemistry - (reply: 3)
36. Zeba buffer exchange low protein yield - (reply: 6)
37. iTRAQ or ELISA? - (reply: 1)
38. 2D Quant kit - (reply: 2)
39. 2D PAGE and Western blotting - (reply: 1)
40. How to detect specific-protein producing cells? - (reply: 8)
41. continual problems with 2d gel electrophoresis - (reply: 3)
42. Gradient centrifugation - (reply: 1)
43. Liquid nitrogen protein extraction - (reply: 3)
44. protein extraction from lettuce - (reply: 1)
45. Protein Clevage - (reply: 1)
46. yeast two hybrid 3-AT - (reply: 1)
47. Protein expression - (reply: 1)
48. Protein denaturation - (reply: 5)
49. Human cell membrane protein Western blot! - (reply: 2)
50. protein staining with EtBr!! - (reply: 4)
51. large scale protein purification - (reply: 6)
52. Insoluble protein after ammonium sulfate precipitation - (reply: 1)
53. General questions for enzyme assay - (reply: 2)
54. Question regarding protein conservation among similar genes - (reply: 2)
55. Detection of SMALL Peptide (5 amino acids in size) - (reply: 1)
56. Transfer buffer for tris tricine gel - (reply: 12)
57. RECOMBINANT PROTEIN - (reply: 2)
58. 0.1-0.25% Sodium Deoxycholate vs 420mM NaCl in TrionX-100 lysis buffer. Which is - (reply: 4)
59. Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)
60. Does ultrasonic probe sonication break up mitochondria or nuclear membrane? - (reply: 2)