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Top : New Forum Archives (2009-): : Protein-and-Proteomics
31. Use of fluorescent stain on IEF gels - (reply: 1)
32. Use of speedvac to dry in-gel peptide digest before sending for LCMS - (reply: 2)
33. doublets on 2D gel - (reply: 4)
34. RNA later and 2D SDS PAGE - (reply: 3)
35. Peptide quantification - (reply: 4)
36. Affinity purification glycine elution chemistry - (reply: 3)
37. Zeba buffer exchange low protein yield - (reply: 6)
38. iTRAQ or ELISA? - (reply: 1)
39. 2D Quant kit - (reply: 2)
40. 2D PAGE and Western blotting - (reply: 1)
41. How to detect specific-protein producing cells? - (reply: 8)
42. continual problems with 2d gel electrophoresis - (reply: 3)
43. Gradient centrifugation - (reply: 1)
44. Liquid nitrogen protein extraction - (reply: 3)
45. protein extraction from lettuce - (reply: 1)
46. Protein Clevage - (reply: 1)
47. yeast two hybrid 3-AT - (reply: 1)
48. Protein expression - (reply: 1)
49. Protein denaturation - (reply: 5)
50. Human cell membrane protein Western blot! - (reply: 2)
51. protein staining with EtBr!! - (reply: 4)
52. large scale protein purification - (reply: 6)
53. Insoluble protein after ammonium sulfate precipitation - (reply: 1)
54. General questions for enzyme assay - (reply: 2)
55. Question regarding protein conservation among similar genes - (reply: 2)
56. Detection of SMALL Peptide (5 amino acids in size) - (reply: 1)
57. Transfer buffer for tris tricine gel - (reply: 12)
58. RECOMBINANT PROTEIN - (reply: 2)
59. 0.1-0.25% Sodium Deoxycholate vs 420mM NaCl in TrionX-100 lysis buffer. Which is - (reply: 4)
60. Cell lysis method to get "intact" nuclear protein complex for CO-IP? - (reply: 1)