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Top : New Forum Archives (2009-): : Protein-and-Proteomics
781. Non-deacetylable Protein? - Is this possible? (reply: 3)
782. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
783. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
784. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
785. EMSA probes - method to make longer probes (reply: 3)
786. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
787. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
788. GST pull down - (reply: 1)
789. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
790. Determining how much enzyme to use based on activity units - (reply: 3)
791. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
792. yield vs concentration of protein - (reply: 3)
793. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
794. Sonication mysteries - (reply: 4)
795. cys rich protein expression - (reply: 1)
796. immunoprecipitation troubleshooting - (reply: 1)
797. Immunoprecipitation with two antibodies - (reply: 10)
798. RIPA Buffer precipitation trouble - (reply: 5)
799. develop western by ECL plus - (reply: 1)
800. Protein extraction from tissue - (reply: 1)
801. problem of membrane protein FPLC - (reply: 1)
802. spot removal from 2D gel and proteomics - help! (reply: 1)
803. Co-IP problem - (reply: 3)
804. I am confused, please help. - (reply: 1)
805. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
806. EMSA using biotin labeled probes and buffers - (reply: 5)
807. Foam in the BSA solution - (reply: 3)
808. Inclusion Body purification - (reply: 2)
809. How to design an antibody - (reply: 1)
810. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)

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