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Top : New Forum Archives (2009-): : Protein-and-Proteomics
781. No enzyme Activity (dioxygenase assay) - (reply: 3)
782. bad western blot! what is the cause? - (reply: 3)
783. N-Terminal Sequencing - (reply: 2)
784. Non-deacetylable Protein? - Is this possible? (reply: 3)
785. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
786. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
787. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
788. EMSA probes - method to make longer probes (reply: 3)
789. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
790. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
791. GST pull down - (reply: 1)
792. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
793. Determining how much enzyme to use based on activity units - (reply: 3)
794. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
795. yield vs concentration of protein - (reply: 3)
796. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
797. Sonication mysteries - (reply: 4)
798. cys rich protein expression - (reply: 1)
799. immunoprecipitation troubleshooting - (reply: 1)
800. Immunoprecipitation with two antibodies - (reply: 10)
801. RIPA Buffer precipitation trouble - (reply: 5)
802. develop western by ECL plus - (reply: 1)
803. Protein extraction from tissue - (reply: 1)
804. problem of membrane protein FPLC - (reply: 1)
805. spot removal from 2D gel and proteomics - help! (reply: 1)
806. Co-IP problem - (reply: 3)
807. I am confused, please help. - (reply: 1)
808. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
809. EMSA using biotin labeled probes and buffers - (reply: 5)
810. Foam in the BSA solution - (reply: 3)

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