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Top : New Forum Archives (2009-): : Protein-and-Proteomics
781. What should be the goal amount of protein to get from purification? - Trying to figure out how much culture I need to make (reply: 3)
782. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
783. DUB Inhibitors - How do they work? (reply: 1)
784. Introduction to x-ray crystallography of proteins - (reply: 1)
785. Determination of purity by SDS PAGE - (reply: 2)
786. Problems with small peptide electrophoresis - (reply: 2)
787. EMSA Shift - (reply: 5)
788. kinase activity - (reply: 1)
789. Western blot materials - sealed plastic (reply: 2)
790. OKADAIC ACID for protein prep - (reply: 1)
791. cells can't be lysed after crosslinking - (reply: 4)
792. Annoying occasional western problem ... anyone have any explanation? - (reply: 4)
793. Can TCA percipitate Glycoprotein? - (reply: 4)
794. Biorad Quantity One software - (reply: 3)
795. Protein concentration for crystallization - (reply: 1)
796. TCA and protein percipitation? - (reply: 2)
797. Constitutively Ubiquitination Mark? - (reply: 3)
798. Co-immunoprecipitation: how to increase amount of interacting protein? - (reply: 4)
799. Brown PVDF membrane after rehydration - (reply: 5)
800. No enzyme Activity (dioxygenase assay) - (reply: 3)
801. bad western blot! what is the cause? - (reply: 3)
802. N-Terminal Sequencing - (reply: 2)
803. Non-deacetylable Protein? - Is this possible? (reply: 3)
804. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
805. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
806. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
807. EMSA probes - method to make longer probes (reply: 3)
808. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
809. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
810. GST pull down - (reply: 1)