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Top : New Forum Archives (2009-): : Protein-and-Proteomics
781. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
782. EMSA probes - method to make longer probes (reply: 3)
783. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
784. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
785. GST pull down - (reply: 1)
786. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
787. Determining how much enzyme to use based on activity units - (reply: 3)
788. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
789. yield vs concentration of protein - (reply: 3)
790. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
791. Sonication mysteries - (reply: 4)
792. cys rich protein expression - (reply: 1)
793. immunoprecipitation troubleshooting - (reply: 1)
794. Immunoprecipitation with two antibodies - (reply: 10)
795. RIPA Buffer precipitation trouble - (reply: 5)
796. develop western by ECL plus - (reply: 1)
797. Protein extraction from tissue - (reply: 1)
798. problem of membrane protein FPLC - (reply: 1)
799. spot removal from 2D gel and proteomics - help! (reply: 1)
800. Co-IP problem - (reply: 3)
801. I am confused, please help. - (reply: 1)
802. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
803. EMSA using biotin labeled probes and buffers - (reply: 5)
804. Foam in the BSA solution - (reply: 3)
805. Inclusion Body purification - (reply: 2)
806. How to design an antibody - (reply: 1)
807. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
808. Protein isolation from skin - (reply: 2)
809. How to concentrate my protein? - (reply: 1)
810. Organism specific protein?? - data from maldi-ms (reply: 6)

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