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Top : New Forum Archives (2009-): : Protein-and-Proteomics
871. NaCl or KCl in hypotonic buffer - (reply: 1)
872. Need a protocol for Native-PAGE - (reply: 3)
873. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
874. competition of thrombin and my protein on benzamidine beads - (reply: 5)
875. how to extract protein from agarose? - (reply: 1)
876. co-IP of chromatin binding protein - (reply: 2)
877. protein in phosphate buffer precipitate out with acetonitrile - (reply: 1)
878. Western blotting for proteins in vesicles? - (reply: 2)
879. His protein expression problem - (reply: 1)
880. Co-IPs and pulldowns of transcription factors - (reply: 2)
881. Yeast transformation - (reply: 1)
882. Scanner type for 2D SDS-PAGE analysis. - A simple and direct question! (reply: 3)
883. Lysis of infected macrophages - without lysing bacteria (reply: 2)
884. Western bands fading out, pleeeese help - (reply: 1)
885. removal of SDS from protein - (reply: 4)
886. prepare sample protein for 2d from E.coli - (reply: 2)
887. Do you add 10% Glycerin in your IP Lysis Buffer - and do you use 1% NP40 or 1% TritonX-100? (reply: 2)
888. Expression of a possible toxic gene? - (reply: 5)
889. Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
890. N-terminal sequencing - (reply: 2)
891. how to explore protein interactions - (reply: 3)
892. Suitable controls for for cellular fractionation - (reply: 2)
893. Q about the housekeeping gene - (reply: 1)
894. Microplate Bradford assay - very urgent (reply: 11)
895. Incomplete denaturing with 8M urea? - (reply: 3)
896. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
897. Out of expected protein size in SDS-PAGE - (reply: 10)
898. Bradford quantitatn problem - precipitation - protein lysate precipitate when added to assay reagent (reply: 1)
899. Sugar solution had changed colour - strange... (reply: 8)
900. storing WB blots in PBS-tween - (reply: 7)