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Top : New Forum Archives (2009-): : Protein-and-Proteomics
871. role of NaCl in lysis buffer - (reply: 2)
872. NaCl or KCl in hypotonic buffer - (reply: 1)
873. Need a protocol for Native-PAGE - (reply: 3)
874. Different mol wt band compared to bioinformatic prediction algorithms - (reply: 4)
875. competition of thrombin and my protein on benzamidine beads - (reply: 5)
876. how to extract protein from agarose? - (reply: 1)
877. co-IP of chromatin binding protein - (reply: 2)
878. protein in phosphate buffer precipitate out with acetonitrile - (reply: 1)
879. Western blotting for proteins in vesicles? - (reply: 2)
880. His protein expression problem - (reply: 1)
881. Co-IPs and pulldowns of transcription factors - (reply: 2)
882. Yeast transformation - (reply: 1)
883. Scanner type for 2D SDS-PAGE analysis. - A simple and direct question! (reply: 3)
884. Lysis of infected macrophages - without lysing bacteria (reply: 2)
885. Western bands fading out, pleeeese help - (reply: 1)
886. removal of SDS from protein - (reply: 4)
887. prepare sample protein for 2d from E.coli - (reply: 2)
888. Do you add 10% Glycerin in your IP Lysis Buffer - and do you use 1% NP40 or 1% TritonX-100? (reply: 2)
889. Expression of a possible toxic gene? - (reply: 5)
890. Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
891. N-terminal sequencing - (reply: 2)
892. how to explore protein interactions - (reply: 3)
893. Suitable controls for for cellular fractionation - (reply: 2)
894. Q about the housekeeping gene - (reply: 1)
895. Microplate Bradford assay - very urgent (reply: 11)
896. Incomplete denaturing with 8M urea? - (reply: 3)
897. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
898. Out of expected protein size in SDS-PAGE - (reply: 10)
899. Bradford quantitatn problem - precipitation - protein lysate precipitate when added to assay reagent (reply: 1)
900. Sugar solution had changed colour - strange... (reply: 8)