1. Protein sequence conservation - (reply: 2)
2. Dye with thiol group - (reply: 1)
3. CoIP - (reply: 2)
4. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
5. Problem in acetone precipitation of plant proteins. - (reply: 1)
6. Biotinylation - (reply: 1)
7. His Tagged Recombinant Protein not Recognized in Western Blot - (reply: 1)
8. Protein folding in different species - (reply: 1)
9. Immunoprecipitation- Incubation time with beads - (reply: 1)
10. desalting resin - (reply: 1)
11. immunoprecipitation concept - (reply: 4)
12. Protein activation after purification - (reply: 4)
13. Co-Immunoprecipitation - Query - (reply: 2)
14. protein extraction and peptide quantification from fat breast tissue - (reply: 1)
15. name of a database to search homologous? - (reply: 1)
16. Peptide HPLC Sample Prep - (reply: 2)
17. 2D Image Primary Cell line - (reply: 13)
18. Quantitation of protein concentration in rehydration buffer - (reply: 3)
19. membrane proteomics - (reply: 1)
20. Membrane proteins on 2D gel electrophoresis..... - (reply: 1)
21. SDS-PAGE sample streaking issue - (reply: 8)
22. Membrane vs. Cytosol Fractionation - (reply: 2)
23. Antibody detection problem - (reply: 1)
24. Protein quantification using HPLC - (reply: 1)
25. Isoelectric focusing help - (reply: 1)
26. fast human protein function catalog? - (reply: 3)
27. In solution digestion - (reply: 2)
28. ProteinCenter alternative? - (reply: 3)
29. low concentration protein purification - (reply: 1)
30. Isoelectric focusing - (reply: 1)

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