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Top : New Forum Archives (2009-): : Protein-and-Proteomics
1021. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
1022. western blotting loading control - (reply: 5)
1023. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
1024. Immunolotting of actin protein coming at higher MW - (reply: 1)
1025. Isolation of secreted proteins from culture media - (reply: 2)
1026. smear on gel after tca precipitation - (reply: 3)
1027. Protein loading marker for glycoproteins - (reply: 2)
1028. distinguish protein expression from high homology - (reply: 1)
1029. (in vitro binding assay w/ IP)- a-Mouse Ab seems to recognize rabbit a-GFP Ab or - (reply: 2)
1030. How to store membrane with primary antibody? - (reply: 5)
1031. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
1032. Weird BSA standard curve for Bradford assay - (reply: 9)
1033. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
1034. Supershift Problems - Nonspecific shifting (reply: 2)
1035. GSK-3b activity - (reply: 2)
1036. Ab purification - (reply: 1)
1037. Alternative methods to detect protein:protein interaction - (reply: 3)
1038. Protein Extraction from Human Tissue/Tumor Samples - (reply: 1)
1039. Protein targetting - (reply: 1)
1040. Problems in His-tag protein purification with TALON - (reply: 1)
1041. SDF-1(CXCL12) western blot - (reply: 13)
1042. SDS gel - (reply: 2)
1043. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
1044. Co-IP hassle - (reply: 5)
1045. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
1046. Extracting Proteins From Soil - (reply: 3)
1047. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
1048. GST preotin non specific preotins - Non specific GST bands (reply: 2)
1049. EMSA and supershift - Tips for supershift after EMSA (reply: 6)
1050. actin MW problem in western blot - (reply: 4)