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Top : New Forum Archives (2009-): : Protein-and-Proteomics
1021. SDS-DNA-PAGE - How to? (reply: 3)
1022. antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
1023. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
1024. western blotting loading control - (reply: 5)
1025. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
1026. Immunolotting of actin protein coming at higher MW - (reply: 1)
1027. Isolation of secreted proteins from culture media - (reply: 2)
1028. smear on gel after tca precipitation - (reply: 3)
1029. Protein loading marker for glycoproteins - (reply: 2)
1030. distinguish protein expression from high homology - (reply: 1)
1031. (in vitro binding assay w/ IP)- a-Mouse Ab seems to recognize rabbit a-GFP Ab or - (reply: 2)
1032. How to store membrane with primary antibody? - (reply: 5)
1033. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
1034. Weird BSA standard curve for Bradford assay - (reply: 9)
1035. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
1036. Supershift Problems - Nonspecific shifting (reply: 2)
1037. GSK-3b activity - (reply: 2)
1038. Ab purification - (reply: 1)
1039. Alternative methods to detect protein:protein interaction - (reply: 3)
1040. Protein Extraction from Human Tissue/Tumor Samples - (reply: 1)
1041. Protein targetting - (reply: 1)
1042. Problems in His-tag protein purification with TALON - (reply: 1)
1043. SDF-1(CXCL12) western blot - (reply: 13)
1044. SDS gel - (reply: 2)
1045. Co-IP problems with band sizes - Different band sizes in input and pulled down (reply: 1)
1046. Co-IP hassle - (reply: 5)
1047. to increase the sensitivity of SDS-PAGE silver staining?? - (reply: 1)
1048. Extracting Proteins From Soil - (reply: 3)
1049. purifying MMP9 - tagging sepharose beads with gelatin (reply: 1)
1050. GST preotin non specific preotins - Non specific GST bands (reply: 2)