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811. Protein synthesis inhibition - (reply: 4)
812. His tag protein purification - (reply: 3)
813. protein extraction from primary cells - (reply: 1)
814. Most strange sds page gel ever!!!!! - (reply: 5)
815. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
816. EDTA as denaturating agent ?? - for proteins.. (reply: 5)
817. GST pull down assay - (reply: 3)
818. Best yeast expression system - (reply: 1)
819. Help_Role of BSA for the protein reconstitution - (reply: 5)
820. BCA Protein Assay Problems - (reply: 1)
821. protein degardation during gel filteration - (reply: 2)
822. lysate fractionation techniques - (reply: 1)
823. Co-IP using magnetic beads, non-specific binding - (reply: 4)
824. Storage of 8M Urea buffer? - I made it two weeks ago? (reply: 3)
825. Protein solubility improvements? - any methods? (reply: 5)
826. SDS-DNA-PAGE - How to? (reply: 3)
827. antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
828. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
829. western blotting loading control - (reply: 5)
830. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
831. Immunolotting of actin protein coming at higher MW - (reply: 1)
832. Isolation of secreted proteins from culture media - (reply: 2)
833. smear on gel after tca precipitation - (reply: 3)
834. Protein loading marker for glycoproteins - (reply: 2)
835. distinguish protein expression from high homology - (reply: 1)
836. (in vitro binding assay w/ IP)- a-Mouse Ab seems to recognize rabbit a-GFP Ab or - (reply: 2)
837. How to store membrane with primary antibody? - (reply: 5)
838. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
839. Weird BSA standard curve for Bradford assay - (reply: 9)
840. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)
812. His tag protein purification - (reply: 3)
813. protein extraction from primary cells - (reply: 1)
814. Most strange sds page gel ever!!!!! - (reply: 5)
815. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
816. EDTA as denaturating agent ?? - for proteins.. (reply: 5)
817. GST pull down assay - (reply: 3)
818. Best yeast expression system - (reply: 1)
819. Help_Role of BSA for the protein reconstitution - (reply: 5)
820. BCA Protein Assay Problems - (reply: 1)
821. protein degardation during gel filteration - (reply: 2)
822. lysate fractionation techniques - (reply: 1)
823. Co-IP using magnetic beads, non-specific binding - (reply: 4)
824. Storage of 8M Urea buffer? - I made it two weeks ago? (reply: 3)
825. Protein solubility improvements? - any methods? (reply: 5)
826. SDS-DNA-PAGE - How to? (reply: 3)
827. antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
828. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
829. western blotting loading control - (reply: 5)
830. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
831. Immunolotting of actin protein coming at higher MW - (reply: 1)
832. Isolation of secreted proteins from culture media - (reply: 2)
833. smear on gel after tca precipitation - (reply: 3)
834. Protein loading marker for glycoproteins - (reply: 2)
835. distinguish protein expression from high homology - (reply: 1)
836. (in vitro binding assay w/ IP)- a-Mouse Ab seems to recognize rabbit a-GFP Ab or - (reply: 2)
837. How to store membrane with primary antibody? - (reply: 5)
838. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
839. Weird BSA standard curve for Bradford assay - (reply: 9)
840. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)