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Top : New Forum Archives (2009-): : Protein-and-Proteomics
811. Scanner type for 2D SDS-PAGE analysis. - A simple and direct question! (reply: 3)
812. Lysis of infected macrophages - without lysing bacteria (reply: 2)
813. Western bands fading out, pleeeese help - (reply: 1)
814. removal of SDS from protein - (reply: 4)
815. prepare sample protein for 2d from E.coli - (reply: 2)
816. Do you add 10% Glycerin in your IP Lysis Buffer - and do you use 1% NP40 or 1% TritonX-100? (reply: 2)
817. Expression of a possible toxic gene? - (reply: 5)
818. Over Expression of GFP tagged L-Asp/ Moesin Proteins - (reply: 5)
819. N-terminal sequencing - (reply: 2)
820. how to explore protein interactions - (reply: 3)
821. Suitable controls for for cellular fractionation - (reply: 2)
822. Q about the housekeeping gene - (reply: 1)
823. Microplate Bradford assay - very urgent (reply: 11)
824. Incomplete denaturing with 8M urea? - (reply: 3)
825. Protein quantification from paraformaldehyde-fixed cells - (reply: 2)
826. Out of expected protein size in SDS-PAGE - (reply: 10)
827. Bradford quantitatn problem - precipitation - protein lysate precipitate when added to assay reagent (reply: 1)
828. Sugar solution had changed colour - strange... (reply: 8)
829. storing WB blots in PBS-tween - (reply: 7)
830. simple pipetting problem - (reply: 1)
831. Storage of overexpressed protein - (reply: 4)
832. HELP_Bradford and protein problems - Different protein concentrations (reply: 1)
833. precipitation of proteins from stained cells - Co-IP failure (reply: 6)
834. Recycling coomassie blue - (reply: 5)
835. Bromophenol blue vs. "Coomassie G250" - Is there a difference in their running location? (reply: 2)
836. problem in the GST fusion protein purification - (reply: 4)
837. PDI western blot - can't reduce the disulfide bonds (reply: 5)
838. how to deal with protein precipitates during dialysis - (reply: 1)
839. iTRAQ cost compared to a DIGE approach - (reply: 1)
840. ideas for Fluorescent tagging of 7Kda bacterial protein. - (reply: 1)

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