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Top : New Forum Archives (2009-): : Protein-and-Proteomics
361. Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
362. Far western blotting - (reply: 5)
363. citrate buffer pH 8 - (reply: 1)
364. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
365. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
366. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
367. getting rid of non specific bands in IP experiments - (reply: 7)
368. loading control antibodies - (reply: 1)
369. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
370. detection of phosphorylation from cell pellet - (reply: 2)
371. how to make a recombinant enzyme refolded - (reply: 2)
372. Quantification of LDL Oxidation - (reply: 1)
373. Concentrating protein of a particular size - (reply: 1)
374. Removing a binding site - (reply: 5)
375. GFP 96-well black plates; plastic lid, sealer or paraffin oil to cover? - (reply: 3)
376. 96 well protein expression - (reply: 1)
377. IEF migration to middle of gel, no separation - (reply: 2)
378. P450 Activity Assay - (reply: 1)
379. Help with iTRAQ-SCX protocol - (reply: 2)
380. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
381. Problem: peptide (MW 2.3KDa) can't dialyze out - (reply: 5)
382. Anti-his antibodies - Noob to protein work - (reply: 10)
383. predict possible protein protein interaction - (reply: 1)
384. Western Blotting - (reply: 10)
385. Reuse of 2-Mercaptoethanol added buffer - (reply: 1)
386. how to make nondenaturing gradient gel - (reply: 2)
387. brain and colon tissue homogenate for WB - (reply: 3)
388. Detection of glycosylations on Western blotted proteins - (reply: 2)
389. Massive contaminant with TCA/acetone precipitation - (reply: 2)
390. western blot loading control - (reply: 2)