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Top : New Forum Archives (2009-): : Protein-and-Proteomics
361. Protein dissolved - After acetone precipitation (reply: 2)
362. Is there any new inhibitor of PI3K - help (reply: 2)
363. freezing adherant cells - (reply: 2)
364. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
365. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
366. Separating protein isoforms and quantifying activity - (reply: 3)
367. Protein refolding - (reply: 2)
368. Suprose6 standard - (reply: 2)
369. his tag purification - Nickel vs other resins (reply: 1)
370. Chromatin Protein IP - (reply: 2)
371. Storage 8M Urea Aliquots at -20C? - (reply: 1)
372. protein pass through column - (reply: 1)
373. ask for advice on peptide mapping - exchange buffer, visual comparision, quality criterion (reply: 7)
374. protein precipitation - isolation of proteins (reply: 1)
375. effect of lysozyme on gst-fusion protein - (reply: 1)
376. Two start codons" the TAG and the gene" - (reply: 1)
377. Peptide competition assay - solvent and buffer (reply: 2)
378. Bradford and triton lysis buffer - (reply: 10)
379. Lamin A/C vs Lamin B1 as a nuclear marker?? - (reply: 2)
380. membrane filters for chromatography buffers - (reply: 1)
381. Bio Rad protein assay for liver homogenate - (reply: 1)
382. Bradford Vs beta-actin for WB protein quantification - (reply: 3)
383. EMSA + WB - Transferring 6% native gel (reply: 1)
384. A problem when preparing 75% dioxane solution - (reply: 1)
385. Regarding SDS PAGE of an oligomeric protein - puzzling !!!!!! (reply: 3)
386. acrylamide gel for low molecular weight protein - (reply: 2)
387. brain tissue preparation for immunoblot - (reply: 1)
388. how many samples should i prepare? - about 2 dimensional electrophoresis (reply: 2)
389. IP looked like it worked - Mass Spec says different - (reply: 3)
390. Identify ponceau visible bands - (reply: 1)

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