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Protein-and-Proteomics
361.
NuPAGE Gel get smaller after staining o/n -
(reply: 1)
362.
Single amino acid substitutions away from the active site causing disease. -
(reply: 4)
363.
how to express protein in soluble form -
(reply: 1)
364.
IP no target protein in elution -
(reply: 1)
365.
Protein stability -
(reply: 4)
366.
Relation between microns and kD -
(reply: 3)
367.
ATP assay - malachite green -
(reply: 3)
368.
PROTEIN QUANTIFICATION - cell lysis buffer
(reply: 2)
369.
Protein isolation in zebrafish -
(reply: 1)
370.
Acetone washes for TCA precipitation protocoles -
(reply: 3)
371.
Biotinylation of protein in tris buffer -
(reply: 2)
372.
HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG
(reply: 1)
373.
Protein kinase protocols -
(reply: 2)
374.
Identification of Membrane Receptor protein, how ?? - I wanna use MS approach !
(reply: 4)
375.
Purification of His tagged protein -
(reply: 2)
376.
Protease and proteasome inhibitors -
(reply: 2)
377.
BCA diluent and standard curve -
(reply: 1)
378.
protein induction problem -
(reply: 4)
379.
Growing insect cells with other cell lines -
(reply: 2)
380.
Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein
(reply: 2)
381.
Protein expression declines after overnight incubation -
(reply: 4)
382.
StrepTag Missing Amino Acid - Protein Expression Tag
(reply: 1)
383.
Help! Cell homogenization and protein extraction for Western Blotting -
(reply: 2)
384.
Fusion protein linker -
(reply: 2)
385.
Nuclear fraction versus cytoplasmic fraction -
(reply: 6)
386.
common reasons proteins show up at different sizes on blot - what are they?
(reply: 1)
387.
Homogenization of articular cartilage prior to protein extraction -
(reply: 1)
388.
Protein streaking on Blue Native-PAGE -
(reply: 9)
389.
basic questions about protein assays and cuvettes -
(reply: 4)
390.
How to attain pure chains of one protein complex -
(reply: 1)
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