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421. GST pulldown: elude or not? - (reply: 1)
422. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
423. secreted protein quantification - (reply: 2)
424. Am. Sulp Precipitation - (reply: 2)
425. EMSA - Probe Annealing problems (reply: 1)
426. Mass spectrometry for dummies? - (reply: 3)
427. Major Protein Loss! Why? - (reply: 2)
428. Filming membrane (Western Blot & ECL) - (reply: 1)
429. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
430. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
431. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
432. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
433. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
434. protein purification - (reply: 5)
435. western blot is not consistent with immunofluorescence - (reply: 2)
436. determination of the Stoichiometry - (reply: 1)
437. cytoplasmic and nuclear extraction for BCA - (reply: 6)
438. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
439. protein prep - about prptein preperation in diagnosis (reply: 1)
440. blocking for 48hours - (reply: 1)
441. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
442. SDS PAGE - Bubble inside the Gel (reply: 5)
443. loss of protein during concentration - (reply: 6)
444. protease inhibitors and bradford - (reply: 2)
445. Cell lysates - Protein precipitation? - (reply: 3)
446. Which lysis buffer should I use? - (reply: 5)
447. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
448. cell lysate for pulldown - (reply: 3)
449. two hybrid system - opinion (reply: 1)
450. How many kDa add a post-translation modification? - (reply: 2)
422. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
423. secreted protein quantification - (reply: 2)
424. Am. Sulp Precipitation - (reply: 2)
425. EMSA - Probe Annealing problems (reply: 1)
426. Mass spectrometry for dummies? - (reply: 3)
427. Major Protein Loss! Why? - (reply: 2)
428. Filming membrane (Western Blot & ECL) - (reply: 1)
429. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
430. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
431. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
432. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
433. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
434. protein purification - (reply: 5)
435. western blot is not consistent with immunofluorescence - (reply: 2)
436. determination of the Stoichiometry - (reply: 1)
437. cytoplasmic and nuclear extraction for BCA - (reply: 6)
438. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
439. protein prep - about prptein preperation in diagnosis (reply: 1)
440. blocking for 48hours - (reply: 1)
441. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
442. SDS PAGE - Bubble inside the Gel (reply: 5)
443. loss of protein during concentration - (reply: 6)
444. protease inhibitors and bradford - (reply: 2)
445. Cell lysates - Protein precipitation? - (reply: 3)
446. Which lysis buffer should I use? - (reply: 5)
447. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
448. cell lysate for pulldown - (reply: 3)
449. two hybrid system - opinion (reply: 1)
450. How many kDa add a post-translation modification? - (reply: 2)