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Top : New Forum Archives (2009-): : Protein-and-Proteomics
421. Unstable expression in BL21 DE3 strain - (reply: 4)
422. YFP protein fusion accumulates - (reply: 10)
423. Dry transfer - (reply: 8)
424. 4-MUG stability - in -20 over 15 years (reply: 1)
425. One Shot TOP10 Cells - (reply: 6)
426. problem of 2D-GE with phagosome fractions - (reply: 7)
427. Blocking beads for immunoprecipitation - (reply: 1)
428. Lactobacillus crude protein extraction? - (reply: 8)
429. Size of Prostaglandin, who knows? - (reply: 1)
430. Fusion tagged protein and Western Blot problem - (reply: 3)
431. Compatible detergents for proteomics - (reply: 4)
432. Immunoprecipitation problem - Protein nonspecific binding to bead (reply: 2)
433. Troubleshooting Protein Purification using FPLC - (reply: 1)
434. Sodium Butyrate Stock Preparation - pH; histone preparation; acetylated histone (reply: 2)
435. Problem with NE-PER cytoplasmatic and nuclear separation kit - (reply: 1)
436. his- & biotin- tag protein purfication - (reply: 2)
437. How to remove protein to show an activity is protein based - (reply: 7)
438. Gel filtration Chromatography - (reply: 1)
439. Western: Mouse antibody on mouse tissue homogenates - (reply: 3)
440. Question about Urea - (reply: 2)
441. help with his tagged protein purification - precipitation problem (reply: 1)
442. Enzymes Technology - Enzymic Hydrolysis (reply: 1)
443. Stacking Gel Polymerization - (reply: 4)
444. Is it possible to see a dimer/oligomer in denaturing SDS-PAGE? - (reply: 1)
445. Quick disapperance of ECL signal - (reply: 1)
446. why phosphorylated protein relative to total protein? - (reply: 2)
447. Protocol for refolding by dilution - (reply: 1)
448. Freezing cell pellets and minimizing lysis - (reply: 2)
449. insoluble fusion protein - (reply: 1)
450. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)

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