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Protein-and-Proteomics
691.
Protein quantification from paraformaldehyde-fixed cells -
(reply: 2)
692.
Out of expected protein size in SDS-PAGE -
(reply: 10)
693.
Bradford quantitatn problem - precipitation - protein lysate precipitate when added to assay reagent
(reply: 1)
694.
Sugar solution had changed colour - strange...
(reply: 8)
695.
storing WB blots in PBS-tween -
(reply: 7)
696.
simple pipetting problem -
(reply: 1)
697.
Storage of overexpressed protein -
(reply: 4)
698.
HELP_Bradford and protein problems - Different protein concentrations
(reply: 1)
699.
precipitation of proteins from stained cells - Co-IP failure
(reply: 6)
700.
Recycling coomassie blue -
(reply: 5)
701.
Bromophenol blue vs. "Coomassie G250" - Is there a difference in their running location?
(reply: 2)
702.
problem in the GST fusion protein purification -
(reply: 4)
703.
PDI western blot - can't reduce the disulfide bonds
(reply: 5)
704.
how to deal with protein precipitates during dialysis -
(reply: 1)
705.
iTRAQ cost compared to a DIGE approach -
(reply: 1)
706.
ideas for Fluorescent tagging of 7Kda bacterial protein. -
(reply: 1)
707.
Concentrating Protein - 8M Urea, Protein solution, concentrating
(reply: 8)
708.
Qubit Protein Quantitation Issues - Qubit protein quantitation
(reply: 2)
709.
Protein extraction from plant leaves, bioactivity -
(reply: 1)
710.
mTOR -
(reply: 4)
711.
BN-PAGE -
(reply: 2)
712.
Co-immunoprecipitation - PI3K
(reply: 10)
713.
LC-MS protocol -
(reply: 7)
714.
SDS PAGE: sample buffer 2-ME or DTT?? -
(reply: 1)
715.
SDS PAGE:no of lanes senstivity?? -
(reply: 1)
716.
Protein Disappeareance -
(reply: 1)
717.
Storing Purified Proteins - Seeking a Preservative
(reply: 6)
718.
Bradford assay+high urea -
(reply: 3)
719.
Extract collagen from rat tail (in lab) -
(reply: 1)
720.
collagen extraction -
(reply: 1)
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