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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. Brown PVDF membrane after rehydration - (reply: 5)
692. No enzyme Activity (dioxygenase assay) - (reply: 3)
693. bad western blot! what is the cause? - (reply: 3)
694. N-Terminal Sequencing - (reply: 2)
695. Non-deacetylable Protein? - Is this possible? (reply: 3)
696. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
697. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
698. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
699. EMSA probes - method to make longer probes (reply: 3)
700. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
701. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
702. GST pull down - (reply: 1)
703. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
704. Determining how much enzyme to use based on activity units - (reply: 3)
705. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
706. yield vs concentration of protein - (reply: 3)
707. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
708. Sonication mysteries - (reply: 4)
709. cys rich protein expression - (reply: 1)
710. immunoprecipitation troubleshooting - (reply: 1)
711. Immunoprecipitation with two antibodies - (reply: 10)
712. RIPA Buffer precipitation trouble - (reply: 5)
713. develop western by ECL plus - (reply: 1)
714. Protein extraction from tissue - (reply: 1)
715. problem of membrane protein FPLC - (reply: 1)
716. spot removal from 2D gel and proteomics - help! (reply: 1)
717. Co-IP problem - (reply: 3)
718. I am confused, please help. - (reply: 1)
719. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
720. EMSA using biotin labeled probes and buffers - (reply: 5)

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