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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. western blot to a metal mesh - (reply: 3)
692. Protein quantification - (reply: 2)
693. Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
694. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
695. Custom Primary Antibody - (reply: 3)
696. substrate for kinase - finding the predicted substrate for a kinase (reply: 6)
697. when should I add IPTG? - (reply: 2)
698. No protein spots in 2D gel - Silver staining! - (reply: 17)
699. appropriate time for induction (adding IPTG) - (reply: 1)
700. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
701. protein crystallography services question - (reply: 1)
702. Probelm with gel filtration - (reply: 4)
703. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
704. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
705. best and simple method to purify protein - (reply: 38)
706. Pipet trypsin tips - (reply: 3)
707. Are proteases a problem in protein quantification assays? - (reply: 3)
708. serum housekeeping proteins for total protein normalization - (reply: 1)
709. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
710. Co-IP on nuclear fraction - (reply: 2)
711. Problem with dot blot - Denatured sample??? (reply: 1)
712. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
713. Odyssey Imaging and EMSA - (reply: 2)
714. 2d page - no band (reply: 2)
715. preparing immunoprecipitated sample for mass spec - (reply: 2)
716. Does Change pH could affect protein interaction - GST pul down (reply: 2)
717. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
718. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 8)
719. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
720. Proteasome assay - (reply: 6)