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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. when should I add IPTG? - (reply: 2)
692. No protein spots in 2D gel - Silver staining! - (reply: 17)
693. appropriate time for induction (adding IPTG) - (reply: 1)
694. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
695. protein crystallography services question - (reply: 1)
696. Probelm with gel filtration - (reply: 4)
697. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
698. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
699. best and simple method to purify protein - (reply: 38)
700. Pipet trypsin tips - (reply: 3)
701. Are proteases a problem in protein quantification assays? - (reply: 3)
702. serum housekeeping proteins for total protein normalization - (reply: 1)
703. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
704. Co-IP on nuclear fraction - (reply: 2)
705. Problem with dot blot - Denatured sample??? (reply: 1)
706. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
707. Odyssey Imaging and EMSA - (reply: 2)
708. 2d page - no band (reply: 2)
709. preparing immunoprecipitated sample for mass spec - (reply: 2)
710. Does Change pH could affect protein interaction - GST pul down (reply: 2)
711. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
712. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 8)
713. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
714. Proteasome assay - (reply: 6)
715. protein extraction - ripa buffer vs urea buffer (reply: 1)
716. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
717. EMSA - probe design and competition - (reply: 6)
718. Internal control - (reply: 1)
719. IP my protein from culture supernatant - (reply: 2)
720. High pI proteins - High pI proteins (reply: 4)