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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. Co-IP on nuclear fraction - (reply: 2)
692. Problem with dot blot - Denatured sample??? (reply: 1)
693. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
694. Odyssey Imaging and EMSA - (reply: 2)
695. 2d page - no band (reply: 2)
696. preparing immunoprecipitated sample for mass spec - (reply: 2)
697. Does Change pH could affect protein interaction - GST pul down (reply: 2)
698. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
699. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 8)
700. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
701. Proteasome assay - (reply: 6)
702. protein extraction - ripa buffer vs urea buffer (reply: 1)
703. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
704. EMSA - probe design and competition - (reply: 6)
705. Internal control - (reply: 1)
706. IP my protein from culture supernatant - (reply: 2)
707. High pI proteins - High pI proteins (reply: 4)
708. antibody purification - ammonium sulfate precipitation - (reply: 7)
709. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
710. Protein-Lipid Overlay Assay - Troubleshooting (reply: 1)
711. Protein Degradation after overexpression - (reply: 1)
712. Southern blot troubleshoot - (reply: 8)
713. IP from nuclear extract - (reply: 3)
714. Circular Dichroism - Can I use NaCl? (reply: 2)
715. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
716. Problems in upscaling protein expression - (reply: 2)
717. amonium precipitation - (reply: 3)
718. preserving bovine serum albumin with sodium azide - (reply: 3)
719. Where is the future of the proteomics? - (reply: 5)
720. BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)

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