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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. How can I get protein in soluble fration - (reply: 2)
692. How long does it takes to make a crystallography of a protein? - (reply: 1)
693. how to detect a knowen protein's receptor? - (reply: 1)
694. protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
695. western blot to a metal mesh - (reply: 3)
696. Protein quantification - (reply: 2)
697. Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
698. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
699. Custom Primary Antibody - (reply: 3)
700. substrate for kinase - finding the predicted substrate for a kinase (reply: 6)
701. when should I add IPTG? - (reply: 2)
702. No protein spots in 2D gel - Silver staining! - (reply: 17)
703. appropriate time for induction (adding IPTG) - (reply: 1)
704. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
705. protein crystallography services question - (reply: 1)
706. Probelm with gel filtration - (reply: 4)
707. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
708. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
709. best and simple method to purify protein - (reply: 38)
710. Pipet trypsin tips - (reply: 3)
711. Are proteases a problem in protein quantification assays? - (reply: 3)
712. serum housekeeping proteins for total protein normalization - (reply: 1)
713. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
714. Co-IP on nuclear fraction - (reply: 2)
715. Problem with dot blot - Denatured sample??? (reply: 1)
716. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
717. Odyssey Imaging and EMSA - (reply: 2)
718. 2d page - no band (reply: 2)
719. preparing immunoprecipitated sample for mass spec - (reply: 2)
720. Does Change pH could affect protein interaction - GST pul down (reply: 2)