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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. appropriate time for induction (adding IPTG) - (reply: 1)
692. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
693. protein crystallography services question - (reply: 1)
694. Probelm with gel filtration - (reply: 4)
695. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
696. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
697. best and simple method to purify protein - (reply: 38)
698. Pipet trypsin tips - (reply: 3)
699. Are proteases a problem in protein quantification assays? - (reply: 3)
700. serum housekeeping proteins for total protein normalization - (reply: 1)
701. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
702. Co-IP on nuclear fraction - (reply: 2)
703. Problem with dot blot - Denatured sample??? (reply: 1)
704. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
705. Odyssey Imaging and EMSA - (reply: 2)
706. 2d page - no band (reply: 2)
707. preparing immunoprecipitated sample for mass spec - (reply: 2)
708. Does Change pH could affect protein interaction - GST pul down (reply: 2)
709. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
710. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 8)
711. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
712. Proteasome assay - (reply: 6)
713. protein extraction - ripa buffer vs urea buffer (reply: 1)
714. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
715. EMSA - probe design and competition - (reply: 6)
716. Internal control - (reply: 1)
717. IP my protein from culture supernatant - (reply: 2)
718. High pI proteins - High pI proteins (reply: 4)
719. antibody purification - ammonium sulfate precipitation - (reply: 7)
720. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)