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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
692. Co-IP on nuclear fraction - (reply: 2)
693. Problem with dot blot - Denatured sample??? (reply: 1)
694. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
695. Odyssey Imaging and EMSA - (reply: 2)
696. 2d page - no band (reply: 2)
697. preparing immunoprecipitated sample for mass spec - (reply: 2)
698. Does Change pH could affect protein interaction - GST pul down (reply: 2)
699. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
700. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 8)
701. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
702. Proteasome assay - (reply: 6)
703. protein extraction - ripa buffer vs urea buffer (reply: 1)
704. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
705. EMSA - probe design and competition - (reply: 6)
706. Internal control - (reply: 1)
707. IP my protein from culture supernatant - (reply: 2)
708. High pI proteins - High pI proteins (reply: 4)
709. antibody purification - ammonium sulfate precipitation - (reply: 7)
710. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
711. Protein-Lipid Overlay Assay - Troubleshooting (reply: 1)
712. Protein Degradation after overexpression - (reply: 1)
713. Southern blot troubleshoot - (reply: 8)
714. IP from nuclear extract - (reply: 3)
715. Circular Dichroism - Can I use NaCl? (reply: 2)
716. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
717. Problems in upscaling protein expression - (reply: 2)
718. amonium precipitation - (reply: 3)
719. preserving bovine serum albumin with sodium azide - (reply: 3)
720. Where is the future of the proteomics? - (reply: 5)

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