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Top : New Forum Archives (2009-): : Protein-and-Proteomics
691. best and simple method to purify protein - (reply: 38)
692. Pipet trypsin tips - (reply: 3)
693. Are proteases a problem in protein quantification assays? - (reply: 3)
694. serum housekeeping proteins for total protein normalization - (reply: 1)
695. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
696. Co-IP on nuclear fraction - (reply: 2)
697. Problem with dot blot - Denatured sample??? (reply: 1)
698. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
699. Odyssey Imaging and EMSA - (reply: 2)
700. 2d page - no band (reply: 2)
701. preparing immunoprecipitated sample for mass spec - (reply: 2)
702. Does Change pH could affect protein interaction - GST pul down (reply: 2)
703. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
704. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 8)
705. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
706. Proteasome assay - (reply: 6)
707. protein extraction - ripa buffer vs urea buffer (reply: 1)
708. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
709. EMSA - probe design and competition - (reply: 6)
710. Internal control - (reply: 1)
711. IP my protein from culture supernatant - (reply: 2)
712. High pI proteins - High pI proteins (reply: 4)
713. antibody purification - ammonium sulfate precipitation - (reply: 7)
714. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
715. Protein-Lipid Overlay Assay - Troubleshooting (reply: 1)
716. Protein Degradation after overexpression - (reply: 1)
717. Southern blot troubleshoot - (reply: 8)
718. IP from nuclear extract - (reply: 3)
719. Circular Dichroism - Can I use NaCl? (reply: 2)
720. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)

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