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451. two hybrid system - opinion (reply: 1)
452. How many kDa add a post-translation modification? - (reply: 2)
453. making gel in advance - to save time (reply: 7)
454. peptide markers for native PAGE - (reply: 3)
455. protein sequencing company - (reply: 2)
456. Protein extraction from mouse prostate - (reply: 3)
457. Immunoprecipitation Troubleshooting - Immunoprecipitation (reply: 7)
458. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
459. fluorescent proteins expressed in nucleus - (reply: 6)
460. two bands detected in western - (reply: 1)
461. Ponceau S dilemma - "Trichloroacetic" or "Trifluoroacetic" acid (reply: 2)
462. Protein SDS-PAGE thick bands / smear - (reply: 4)
463. negative control in IP - (reply: 2)
464. Replacement of Zinc sulphate in Zinc staining - by using zinc chloride (reply: 2)
465. EMSA gels - (reply: 2)
466. gst pulldown - (reply: 2)
467. Co-IP - (reply: 2)
468. Across blot comparisons - (reply: 2)
469. Western blot data analysis - (reply: 1)
470. Native molecular weight determination - using restricted SDS in PAGE (reply: 2)
471. Lost protein after gel filtration - Gel Filtration (reply: 6)
472. Colorimetric kinase assay - (reply: 2)
473. nuclear extraction problem - nuclei gets too clumpy - (reply: 3)
474. protein size - (reply: 1)
475. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
476. immunoprecipitation - immunoprecipitation help needed (reply: 1)
477. estimate size of protein - (reply: 2)
478. Problem- wierd 2D gel image - (reply: 5)
479. Protein elution from GST-fusion protein complex - (reply: 3)
480. total cell protein vs membrane protein - (reply: 4)
452. How many kDa add a post-translation modification? - (reply: 2)
453. making gel in advance - to save time (reply: 7)
454. peptide markers for native PAGE - (reply: 3)
455. protein sequencing company - (reply: 2)
456. Protein extraction from mouse prostate - (reply: 3)
457. Immunoprecipitation Troubleshooting - Immunoprecipitation (reply: 7)
458. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
459. fluorescent proteins expressed in nucleus - (reply: 6)
460. two bands detected in western - (reply: 1)
461. Ponceau S dilemma - "Trichloroacetic" or "Trifluoroacetic" acid (reply: 2)
462. Protein SDS-PAGE thick bands / smear - (reply: 4)
463. negative control in IP - (reply: 2)
464. Replacement of Zinc sulphate in Zinc staining - by using zinc chloride (reply: 2)
465. EMSA gels - (reply: 2)
466. gst pulldown - (reply: 2)
467. Co-IP - (reply: 2)
468. Across blot comparisons - (reply: 2)
469. Western blot data analysis - (reply: 1)
470. Native molecular weight determination - using restricted SDS in PAGE (reply: 2)
471. Lost protein after gel filtration - Gel Filtration (reply: 6)
472. Colorimetric kinase assay - (reply: 2)
473. nuclear extraction problem - nuclei gets too clumpy - (reply: 3)
474. protein size - (reply: 1)
475. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
476. immunoprecipitation - immunoprecipitation help needed (reply: 1)
477. estimate size of protein - (reply: 2)
478. Problem- wierd 2D gel image - (reply: 5)
479. Protein elution from GST-fusion protein complex - (reply: 3)
480. total cell protein vs membrane protein - (reply: 4)