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Top : New Forum Archives (2009-): : Protein-and-Proteomics
451. Reasons for mis-expression - (reply: 1)
452. RNA protein complex interaction in 4-12% NU PAGE gradient gel - RNA PROTEIN bEHAVIOR IN GRADIENT GELS (reply: 1)
453. cytoplasmic and nuclear extraction - problem with contamination! - (reply: 2)
454. Removing MBP tag - (reply: 5)
455. methods for protein purification - hi.. can i know different method used for protein purification (reply: 1)
456. EMSA - protein-probe complex stuck in wells? (reply: 1)
457. reducing agent? - (reply: 3)
458. Protein structure prediction - (reply: 2)
459. HSP 104 - (reply: 5)
460. succinate dehydrogenase - (reply: 2)
461. protein precipitate at pI?? - (reply: 1)
462. Does tritonX100 disturbs sds page? - (reply: 1)
463. Protein dissolved - After acetone precipitation (reply: 2)
464. Is there any new inhibitor of PI3K - help (reply: 2)
465. freezing adherant cells - (reply: 2)
466. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
467. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
468. Cysteine Measurement - Levels of intracellular cysteins (reply: 1)
469. Separating protein isoforms and quantifying activity - (reply: 3)
470. Protein refolding - (reply: 2)
471. Suprose6 standard - (reply: 2)
472. his tag purification - Nickel vs other resins (reply: 1)
473. Chromatin Protein IP - (reply: 2)
474. Storage 8M Urea Aliquots at -20C? - (reply: 1)
475. protein pass through column - (reply: 1)
476. ask for advice on peptide mapping - exchange buffer, visual comparision, quality criterion (reply: 7)
477. protein precipitation - isolation of proteins (reply: 1)
478. effect of lysozyme on gst-fusion protein - (reply: 1)
479. Two start codons" the TAG and the gene" - (reply: 1)
480. Peptide competition assay - solvent and buffer (reply: 2)