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Top : New Forum Archives (2009-): : Protein-and-Proteomics
451. freezing adherant cells - (reply: 2)
452. Total cell lysate from T cells - hard to lyze all the nuclei with RIPA (reply: 2)
453. How the heck does serum activate Akt?!? - Is serum starvation necessary in endothelial cells? (reply: 1)
454. Separating protein isoforms and quantifying activity - (reply: 3)
455. Protein refolding - (reply: 2)
456. Suprose6 standard - (reply: 2)
457. his tag purification - Nickel vs other resins (reply: 1)
458. Chromatin Protein IP - (reply: 2)
459. Storage 8M Urea Aliquots at -20C? - (reply: 1)
460. protein pass through column - (reply: 1)
461. ask for advice on peptide mapping - exchange buffer, visual comparision, quality criterion (reply: 7)
462. protein precipitation - isolation of proteins (reply: 1)
463. effect of lysozyme on gst-fusion protein - (reply: 1)
464. Two start codons" the TAG and the gene" - (reply: 1)
465. Peptide competition assay - solvent and buffer (reply: 2)
466. Bradford and triton lysis buffer - (reply: 10)
467. Lamin A/C vs Lamin B1 as a nuclear marker?? - (reply: 2)
468. membrane filters for chromatography buffers - (reply: 1)
469. Bio Rad protein assay for liver homogenate - (reply: 1)
470. Bradford Vs beta-actin for WB protein quantification - (reply: 3)
471. EMSA + WB - Transferring 6% native gel (reply: 1)
472. A problem when preparing 75% dioxane solution - (reply: 1)
473. Regarding SDS PAGE of an oligomeric protein - puzzling !!!!!! (reply: 3)
474. acrylamide gel for low molecular weight protein - (reply: 2)
475. brain tissue preparation for immunoblot - (reply: 1)
476. how many samples should i prepare? - about 2 dimensional electrophoresis (reply: 2)
477. IP looked like it worked - Mass Spec says different - (reply: 3)
478. Identify ponceau visible bands - (reply: 1)
479. plant protein purification opinion - (reply: 3)
480. what is protein flexibility - (reply: 1)

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