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Top : New Forum Archives (2009-): : Protein-and-Proteomics
91. Thrombin inactivation - (reply: 2)
92. IP - crosslinking antibody to dynabeads vs. NHS-acitvated beads? - (reply: 2)
93. Can anyone give a protocol to screen levels of adenosine deaminase in serum samp - (reply: 2)
94. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ri - (reply: 1)
95. half life of a protein - (reply: 3)
96. Western blot using blood plasma - (reply: 5)
97. PelB Leader Sequence Cleavage - (reply: 1)
98. Probing protein - (reply: 1)
99. production of phosphorylated proteins in E.coli host system - (reply: 1)
100. What is the reason of doing proteomics work? - (reply: 2)
101. Protein quantification - (reply: 4)
102. Protein extraction for 2D electrophoresis - (reply: 2)
103. Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
104. Test for protein in lysate - (reply: 4)
105. co-IP stained with coomassie blue - (reply: 2)
106. Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
107. How many PBMCs should I seed so that I can get enough protein? - (reply: 1)
108. co-ip of endogenous and tagged proteins - (reply: 1)
109. Histone extraction from S.cerevisiae - (reply: 1)
110. coomassie blue stain - (reply: 1)
111. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
112. concentation calculations - (reply: 1)
113. Vesicular Monoamine Transporter2 (VMAT2) - (reply: 8)
114. Protein sequence conservation - (reply: 2)
115. MALDI MS resutls to identify proteins - (reply: 2)
116. Dye with thiol group - (reply: 1)
117. CoIP - (reply: 2)
118. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)
119. Problem in acetone precipitation of plant proteins. - (reply: 1)
120. Biotinylation - (reply: 1)

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