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Protein-and-Proteomics
391.
Problems with precipitation of proteins using TCA/aceton -
(reply: 3)
392.
Fusion Protein -
(reply: 3)
393.
Problem with SDS-PAGE, frowny faces everywhere -
(reply: 7)
394.
Need to find a method of detection for free Histag -
(reply: 1)
395.
Blue Native PAGE - Problems with westerns of Blue Native Gels
(reply: 3)
396.
CoIP using Ni NTA, my non-his-tagged protein bind to beads! -
(reply: 1)
397.
High mRNA but no protein expression -
(reply: 10)
398.
Running a western with cell media in gels? -
(reply: 11)
399.
Transfer buffer as running buffer? -
(reply: 7)
400.
nuclear extraction insoluble pellet problem - here it is again!
(reply: 2)
401.
high background after ECL exposition -
(reply: 3)
402.
Circular Dichroism and disordered proteins -
(reply: 1)
403.
2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells
(reply: 1)
404.
inactive enzyme from purification -
(reply: 1)
405.
Please comment on this Co-ip method -
(reply: 2)
406.
protein phosphorylation - methods for protein phosphorylation
(reply: 9)
407.
Co-IP of secreted proteins -
(reply: 1)
408.
Are protease inhibitors overrated ? -
(reply: 2)
409.
gst- pulldown -
(reply: 1)
410.
what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production?
(reply: 1)
411.
Refrigerator left open.....is everything ruined?? :( -
(reply: 5)
412.
143 kDa protein -
(reply: 3)
413.
Do you get insoluble proteins also in whole cell lysate? -
(reply: 3)
414.
Purification od insoluble protein (TIMP3) -
(reply: 2)
415.
descrepancy between total cell lysate and cell fractionation -
(reply: 1)
416.
Immunopricipitation for lysate only? -
(reply: 3)
417.
eluting gst fusion proteins -
(reply: 1)
418.
How to pull down chromatin and identify protein associated with it? -
(reply: 1)
419.
expression at 30 C -
(reply: 1)
420.
Protein extraction from perfusion fixed brain tissue -
(reply: 5)
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