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Top : New Forum Archives (2009-): : Protein-and-Proteomics
991. Protein synthesis inhibition - (reply: 4)
992. His tag protein purification - (reply: 3)
993. protein extraction from primary cells - (reply: 1)
994. Most strange sds page gel ever!!!!! - (reply: 5)
995. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
996. EDTA as denaturating agent ?? - for proteins.. (reply: 5)
997. GST pull down assay - (reply: 3)
998. Best yeast expression system - (reply: 1)
999. Help_Role of BSA for the protein reconstitution - (reply: 5)
1000. BCA Protein Assay Problems - (reply: 1)
1001. protein degardation during gel filteration - (reply: 2)
1002. lysate fractionation techniques - (reply: 1)
1003. Co-IP using magnetic beads, non-specific binding - (reply: 4)
1004. Storage of 8M Urea buffer? - I made it two weeks ago? (reply: 3)
1005. Protein solubility improvements? - any methods? (reply: 5)
1006. SDS-DNA-PAGE - How to? (reply: 3)
1007. antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
1008. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
1009. western blotting loading control - (reply: 5)
1010. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
1011. Immunolotting of actin protein coming at higher MW - (reply: 1)
1012. Isolation of secreted proteins from culture media - (reply: 2)
1013. smear on gel after tca precipitation - (reply: 3)
1014. Protein loading marker for glycoproteins - (reply: 2)
1015. distinguish protein expression from high homology - (reply: 1)
1016. (in vitro binding assay w/ IP)- a-Mouse Ab seems to recognize rabbit a-GFP Ab or - (reply: 2)
1017. How to store membrane with primary antibody? - (reply: 5)
1018. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
1019. Weird BSA standard curve for Bradford assay - (reply: 9)
1020. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)

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