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Top : New Forum Archives (2009-): : Protein-and-Proteomics
991. Protein solubilisation for iTRAQ protocol/general protein solubilisation - (reply: 2)
992. PVDF blot storage - (reply: 3)
993. Too bright blue for bradford assay - (reply: 3)
994. 2D electrophoresis - beta-lactoglobulin (reply: 1)
995. precipitation during dialysis - (reply: 5)
996. Protein stability in RIPA buffer at room temp - (reply: 3)
997. Protein synthesis inhibition - (reply: 4)
998. His tag protein purification - (reply: 3)
999. protein extraction from primary cells - (reply: 1)
1000. Most strange sds page gel ever!!!!! - (reply: 5)
1001. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
1002. EDTA as denaturating agent ?? - for proteins.. (reply: 5)
1003. GST pull down assay - (reply: 3)
1004. Best yeast expression system - (reply: 1)
1005. Help_Role of BSA for the protein reconstitution - (reply: 5)
1006. BCA Protein Assay Problems - (reply: 1)
1007. protein degardation during gel filteration - (reply: 2)
1008. lysate fractionation techniques - (reply: 1)
1009. Co-IP using magnetic beads, non-specific binding - (reply: 4)
1010. Storage of 8M Urea buffer? - I made it two weeks ago? (reply: 3)
1011. Protein solubility improvements? - any methods? (reply: 5)
1012. SDS-DNA-PAGE - How to? (reply: 3)
1013. antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
1014. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
1015. western blotting loading control - (reply: 5)
1016. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
1017. Immunolotting of actin protein coming at higher MW - (reply: 1)
1018. Isolation of secreted proteins from culture media - (reply: 2)
1019. smear on gel after tca precipitation - (reply: 3)
1020. Protein loading marker for glycoproteins - (reply: 2)

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