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Top : New Forum Archives (2009-): : Protein-and-Proteomics
721. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
722. DUB Inhibitors - How do they work? (reply: 1)
723. Introduction to x-ray crystallography of proteins - (reply: 1)
724. Determination of purity by SDS PAGE - (reply: 2)
725. Problems with small peptide electrophoresis - (reply: 2)
726. EMSA Shift - (reply: 5)
727. kinase activity - (reply: 1)
728. Western blot materials - sealed plastic (reply: 2)
729. OKADAIC ACID for protein prep - (reply: 1)
730. cells can't be lysed after crosslinking - (reply: 4)
731. Annoying occasional western problem ... anyone have any explanation? - (reply: 4)
732. Can TCA percipitate Glycoprotein? - (reply: 4)
733. Biorad Quantity One software - (reply: 3)
734. Protein concentration for crystallization - (reply: 1)
735. TCA and protein percipitation? - (reply: 2)
736. Constitutively Ubiquitination Mark? - (reply: 3)
737. Co-immunoprecipitation: how to increase amount of interacting protein? - (reply: 4)
738. Brown PVDF membrane after rehydration - (reply: 5)
739. No enzyme Activity (dioxygenase assay) - (reply: 3)
740. bad western blot! what is the cause? - (reply: 3)
741. N-Terminal Sequencing - (reply: 2)
742. Non-deacetylable Protein? - Is this possible? (reply: 3)
743. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
744. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
745. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
746. EMSA probes - method to make longer probes (reply: 3)
747. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
748. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
749. GST pull down - (reply: 1)
750. Help me convince my boss... - Calling all western blot gurus! (reply: 3)

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