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721. Problem with dot blot - Denatured sample??? (reply: 1)
722. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
723. Odyssey Imaging and EMSA - (reply: 2)
724. 2d page - no band (reply: 2)
725. preparing immunoprecipitated sample for mass spec - (reply: 2)
726. Does Change pH could affect protein interaction - GST pul down (reply: 2)
727. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
728. ELISA peptide coating problem =( - I don’t know what to do to bound the peptide to the plates. (reply: 8)
729. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
730. Proteasome assay - (reply: 6)
731. protein extraction - ripa buffer vs urea buffer (reply: 1)
732. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
733. EMSA - probe design and competition - (reply: 6)
734. Internal control - (reply: 1)
735. IP my protein from culture supernatant - (reply: 2)
736. High pI proteins - High pI proteins (reply: 4)
737. antibody purification - ammonium sulfate precipitation - (reply: 7)
738. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
739. Protein-Lipid Overlay Assay - Troubleshooting (reply: 1)
740. Protein Degradation after overexpression - (reply: 1)
741. Southern blot troubleshoot - (reply: 8)
742. IP from nuclear extract - (reply: 3)
743. Circular Dichroism - Can I use NaCl? (reply: 2)
744. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
745. Problems in upscaling protein expression - (reply: 2)
746. amonium precipitation - (reply: 3)
747. preserving bovine serum albumin with sodium azide - (reply: 3)
748. Where is the future of the proteomics? - (reply: 5)
749. BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)
750. Tris acetate sds buffer - (reply: 2)
722. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
723. Odyssey Imaging and EMSA - (reply: 2)
724. 2d page - no band (reply: 2)
725. preparing immunoprecipitated sample for mass spec - (reply: 2)
726. Does Change pH could affect protein interaction - GST pul down (reply: 2)
727. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
728. ELISA peptide coating problem =( - I don’t know what to do to bound the peptide to the plates. (reply: 8)
729. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
730. Proteasome assay - (reply: 6)
731. protein extraction - ripa buffer vs urea buffer (reply: 1)
732. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
733. EMSA - probe design and competition - (reply: 6)
734. Internal control - (reply: 1)
735. IP my protein from culture supernatant - (reply: 2)
736. High pI proteins - High pI proteins (reply: 4)
737. antibody purification - ammonium sulfate precipitation - (reply: 7)
738. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
739. Protein-Lipid Overlay Assay - Troubleshooting (reply: 1)
740. Protein Degradation after overexpression - (reply: 1)
741. Southern blot troubleshoot - (reply: 8)
742. IP from nuclear extract - (reply: 3)
743. Circular Dichroism - Can I use NaCl? (reply: 2)
744. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
745. Problems in upscaling protein expression - (reply: 2)
746. amonium precipitation - (reply: 3)
747. preserving bovine serum albumin with sodium azide - (reply: 3)
748. Where is the future of the proteomics? - (reply: 5)
749. BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)
750. Tris acetate sds buffer - (reply: 2)