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Top : New Forum Archives (2009-): : Protein-and-Proteomics
721. Protein concentration for crystallization - (reply: 1)
722. TCA and protein percipitation? - (reply: 2)
723. Constitutively Ubiquitination Mark? - (reply: 3)
724. Co-immunoprecipitation: how to increase amount of interacting protein? - (reply: 4)
725. Brown PVDF membrane after rehydration - (reply: 5)
726. No enzyme Activity (dioxygenase assay) - (reply: 3)
727. bad western blot! what is the cause? - (reply: 3)
728. N-Terminal Sequencing - (reply: 2)
729. Non-deacetylable Protein? - Is this possible? (reply: 3)
730. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
731. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
732. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
733. EMSA probes - method to make longer probes (reply: 3)
734. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
735. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
736. GST pull down - (reply: 1)
737. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
738. Determining how much enzyme to use based on activity units - (reply: 3)
739. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
740. yield vs concentration of protein - (reply: 3)
741. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
742. Sonication mysteries - (reply: 4)
743. cys rich protein expression - (reply: 1)
744. immunoprecipitation troubleshooting - (reply: 1)
745. Immunoprecipitation with two antibodies - (reply: 10)
746. RIPA Buffer precipitation trouble - (reply: 5)
747. develop western by ECL plus - (reply: 1)
748. Protein extraction from tissue - (reply: 1)
749. problem of membrane protein FPLC - (reply: 1)
750. spot removal from 2D gel and proteomics - help! (reply: 1)

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