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Top : New Forum Archives (2009-): : Protein-and-Proteomics
721. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
722. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
723. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
724. EMSA probes - method to make longer probes (reply: 3)
725. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
726. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
727. GST pull down - (reply: 1)
728. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
729. Determining how much enzyme to use based on activity units - (reply: 3)
730. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
731. yield vs concentration of protein - (reply: 3)
732. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
733. Sonication mysteries - (reply: 4)
734. cys rich protein expression - (reply: 1)
735. immunoprecipitation troubleshooting - (reply: 1)
736. Immunoprecipitation with two antibodies - (reply: 10)
737. RIPA Buffer precipitation trouble - (reply: 5)
738. develop western by ECL plus - (reply: 1)
739. Protein extraction from tissue - (reply: 1)
740. problem of membrane protein FPLC - (reply: 1)
741. spot removal from 2D gel and proteomics - help! (reply: 1)
742. Co-IP problem - (reply: 3)
743. I am confused, please help. - (reply: 1)
744. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
745. EMSA using biotin labeled probes and buffers - (reply: 5)
746. Foam in the BSA solution - (reply: 3)
747. Inclusion Body purification - (reply: 2)
748. How to design an antibody - (reply: 1)
749. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
750. Protein isolation from skin - (reply: 2)

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