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Top : New Forum Archives (2009-): : Protein-and-Proteomics
721. bad western blot! what is the cause? - (reply: 3)
722. N-Terminal Sequencing - (reply: 2)
723. Non-deacetylable Protein? - Is this possible? (reply: 3)
724. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
725. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
726. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
727. EMSA probes - method to make longer probes (reply: 3)
728. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
729. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
730. GST pull down - (reply: 1)
731. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
732. Determining how much enzyme to use based on activity units - (reply: 3)
733. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
734. yield vs concentration of protein - (reply: 3)
735. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
736. Sonication mysteries - (reply: 4)
737. cys rich protein expression - (reply: 1)
738. immunoprecipitation troubleshooting - (reply: 1)
739. Immunoprecipitation with two antibodies - (reply: 10)
740. RIPA Buffer precipitation trouble - (reply: 5)
741. develop western by ECL plus - (reply: 1)
742. Protein extraction from tissue - (reply: 1)
743. problem of membrane protein FPLC - (reply: 1)
744. spot removal from 2D gel and proteomics - help! (reply: 1)
745. Co-IP problem - (reply: 3)
746. I am confused, please help. - (reply: 1)
747. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
748. EMSA using biotin labeled probes and buffers - (reply: 5)
749. Foam in the BSA solution - (reply: 3)
750. Inclusion Body purification - (reply: 2)

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