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Top : New Forum Archives (2009-): : Protein-and-Proteomics
271. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
272. Role of Isoleucine in protein structure. - (reply: 1)
273. co-IP after ubiquitin cleavage - (reply: 5)
274. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
275. Peptide Conc @215 and 225nm? - (reply: 1)
276. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
277. Detection method of insulin level from blood serum - (reply: 2)
278. Relationship between total protein and enzyme activity - (reply: 3)
279. about cross link the antibody to ProteinG - (reply: 1)
280. EMSA supershift problem - (reply: 1)
281. Co-IP: Prey Protein Present in Neg and Pos Eluates - (reply: 5)
282. covalent-bound HIS complex can not be pull down: help needed - (reply: 2)
283. Protein extraction from mouse tissue? - (reply: 1)
284. Including "M" and "STOP" codons in my insert - (reply: 2)
285. Coomassie stain forzen and thawed gel? - (reply: 1)
286. IEF gel detaching during run - (reply: 7)
287. Overexpressed protein shows a band higher than expected?! - (reply: 2)
288. Bradford Protein Assay - Problem in BSA Standard Curve - (reply: 2)
289. HIS elution: can imidazole be added after SDS-sample buffer? - (reply: 1)
290. What is Pellet that forms AFTER quick spin of boiled lysates? - (reply: 1)
291. ethanol precipitation of protein - (reply: 1)
292. How to thoroughly solubilize proteins from tissue sample, for quantitation - (reply: 1)
293. Protein Concentration from Bacterial Lysates: Help! - (reply: 2)
294. Is DTT reduction and alkylation critical before concentrating proteins? - (reply: 1)
295. Tricine SDS-PAGE - (reply: 3)
296. Pichia growth in MGY/MGYH media - (reply: 1)
297. protien purification using flag affinity - (reply: 4)
298. Constitutive p42/44 MAPK phosphorylation - (reply: 2)
299. His Tag Cleavage (protein precipitates) - (reply: 3)
300. Lysis buffer for Co-IP - (reply: 2)

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