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Top : New Forum Archives (2009-): : Protein-and-Proteomics
631. No protein spots in 2D gel - Silver staining! - (reply: 17)
632. appropriate time for induction (adding IPTG) - (reply: 1)
633. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
634. protein crystallography services question - (reply: 1)
635. Probelm with gel filtration - (reply: 4)
636. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
637. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
638. best and simple method to purify protein - (reply: 38)
639. Pipet trypsin tips - (reply: 3)
640. Are proteases a problem in protein quantification assays? - (reply: 3)
641. serum housekeeping proteins for total protein normalization - (reply: 1)
642. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
643. Co-IP on nuclear fraction - (reply: 2)
644. Problem with dot blot - Denatured sample??? (reply: 1)
645. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
646. Odyssey Imaging and EMSA - (reply: 2)
647. 2d page - no band (reply: 2)
648. preparing immunoprecipitated sample for mass spec - (reply: 2)
649. Does Change pH could affect protein interaction - GST pul down (reply: 2)
650. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
651. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 7)
652. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
653. Proteasome assay - (reply: 6)
654. protein extraction - ripa buffer vs urea buffer (reply: 1)
655. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
656. EMSA - probe design and competition - (reply: 6)
657. Internal control - (reply: 1)
658. IP my protein from culture supernatant - (reply: 2)
659. High pI proteins - High pI proteins (reply: 4)
660. antibody purification - ammonium sulfate precipitation - (reply: 7)

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