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Top : New Forum Archives (2009-): : Protein-and-Proteomics
631. Cell lysates - Protein precipitation? - (reply: 3)
632. Which lysis buffer should I use? - (reply: 5)
633. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
634. cell lysate for pulldown - (reply: 3)
635. two hybrid system - opinion (reply: 1)
636. How many kDa add a post-translation modification? - (reply: 2)
637. making gel in advance - to save time (reply: 7)
638. peptide markers for native PAGE - (reply: 3)
639. protein sequencing company - (reply: 2)
640. Protein extraction from mouse prostate - (reply: 3)
641. Immunoprecipitation Troubleshooting - Immunoprecipitation (reply: 7)
642. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
643. fluorescent proteins expressed in nucleus - (reply: 6)
644. two bands detected in western - (reply: 1)
645. Ponceau S dilemma - "Trichloroacetic" or "Trifluoroacetic" acid (reply: 2)
646. Protein SDS-PAGE thick bands / smear - (reply: 4)
647. negative control in IP - (reply: 2)
648. Replacement of Zinc sulphate in Zinc staining - by using zinc chloride (reply: 2)
649. EMSA gels - (reply: 2)
650. gst pulldown - (reply: 2)
651. Co-IP - (reply: 2)
652. Across blot comparisons - (reply: 2)
653. Western blot data analysis - (reply: 1)
654. Native molecular weight determination - using restricted SDS in PAGE (reply: 2)
655. Lost protein after gel filtration - Gel Filtration (reply: 6)
656. Colorimetric kinase assay - (reply: 2)
657. nuclear extraction problem - nuclei gets too clumpy - (reply: 3)
658. protein size - (reply: 1)
659. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
660. immunoprecipitation - immunoprecipitation help needed (reply: 1)

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