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Top : New Forum Archives (2009-): : Protein-and-Proteomics
631. Are proteases a problem in protein quantification assays? - (reply: 3)
632. serum housekeeping proteins for total protein normalization - (reply: 1)
633. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
634. Co-IP on nuclear fraction - (reply: 2)
635. Problem with dot blot - Denatured sample??? (reply: 1)
636. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
637. Odyssey Imaging and EMSA - (reply: 2)
638. 2d page - no band (reply: 2)
639. preparing immunoprecipitated sample for mass spec - (reply: 2)
640. Does Change pH could affect protein interaction - GST pul down (reply: 2)
641. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
642. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 7)
643. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
644. Proteasome assay - (reply: 6)
645. protein extraction - ripa buffer vs urea buffer (reply: 1)
646. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
647. EMSA - probe design and competition - (reply: 6)
648. Internal control - (reply: 1)
649. IP my protein from culture supernatant - (reply: 2)
650. High pI proteins - High pI proteins (reply: 4)
651. antibody purification - ammonium sulfate precipitation - (reply: 7)
652. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
653. Protein-Lipid Overlay Assay - Troubleshooting (reply: 1)
654. Protein Degradation after overexpression - (reply: 1)
655. Southern blot troubleshoot - (reply: 8)
656. IP from nuclear extract - (reply: 3)
657. Circular Dichroism - Can I use NaCl? (reply: 2)
658. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
659. Problems in upscaling protein expression - (reply: 2)
660. amonium precipitation - (reply: 3)

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