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Top : New Forum Archives (2009-): : Protein-and-Proteomics
631. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
632. cell lysate for pulldown - (reply: 3)
633. two hybrid system - opinion (reply: 1)
634. How many kDa add a post-translation modification? - (reply: 2)
635. making gel in advance - to save time (reply: 7)
636. peptide markers for native PAGE - (reply: 3)
637. protein sequencing company - (reply: 2)
638. Protein extraction from mouse prostate - (reply: 3)
639. Immunoprecipitation Troubleshooting - Immunoprecipitation (reply: 7)
640. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
641. fluorescent proteins expressed in nucleus - (reply: 6)
642. two bands detected in western - (reply: 1)
643. Ponceau S dilemma - "Trichloroacetic" or "Trifluoroacetic" acid (reply: 2)
644. Protein SDS-PAGE thick bands / smear - (reply: 4)
645. negative control in IP - (reply: 2)
646. Replacement of Zinc sulphate in Zinc staining - by using zinc chloride (reply: 2)
647. EMSA gels - (reply: 2)
648. gst pulldown - (reply: 2)
649. Co-IP - (reply: 2)
650. Across blot comparisons - (reply: 2)
651. Western blot data analysis - (reply: 1)
652. Native molecular weight determination - using restricted SDS in PAGE (reply: 2)
653. Lost protein after gel filtration - Gel Filtration (reply: 6)
654. Colorimetric kinase assay - (reply: 2)
655. nuclear extraction problem - nuclei gets too clumpy - (reply: 3)
656. protein size - (reply: 1)
657. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
658. immunoprecipitation - immunoprecipitation help needed (reply: 1)
659. estimate size of protein - (reply: 2)
660. Problem- wierd 2D gel image - (reply: 5)

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