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Top : New Forum Archives (2009-): : Protein-and-Proteomics
661. 3x flag tagged cloning -> western -> multiple bands but not non-specific - Please help me!! (reply: 1)
662. DUB Inhibitors - How do they work? (reply: 1)
663. Introduction to x-ray crystallography of proteins - (reply: 1)
664. Determination of purity by SDS PAGE - (reply: 2)
665. Problems with small peptide electrophoresis - (reply: 2)
666. EMSA Shift - (reply: 5)
667. kinase activity - (reply: 1)
668. Western blot materials - sealed plastic (reply: 2)
669. OKADAIC ACID for protein prep - (reply: 1)
670. cells can't be lysed after crosslinking - (reply: 4)
671. Annoying occasional western problem ... anyone have any explanation? - (reply: 4)
672. Can TCA percipitate Glycoprotein? - (reply: 4)
673. Biorad Quantity One software - (reply: 3)
674. Protein concentration for crystallization - (reply: 1)
675. TCA and protein percipitation? - (reply: 2)
676. Constitutively Ubiquitination Mark? - (reply: 3)
677. Co-immunoprecipitation: how to increase amount of interacting protein? - (reply: 4)
678. Brown PVDF membrane after rehydration - (reply: 5)
679. No enzyme Activity (dioxygenase assay) - (reply: 3)
680. bad western blot! what is the cause? - (reply: 3)
681. N-Terminal Sequencing - (reply: 2)
682. Non-deacetylable Protein? - Is this possible? (reply: 3)
683. Does Laemmli buffer depolymerize actin/microtubules? - (reply: 1)
684. formaldehyde crosslinking for protein-protein interaction - (reply: 1)
685. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
686. EMSA probes - method to make longer probes (reply: 3)
687. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
688. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
689. GST pull down - (reply: 1)
690. Help me convince my boss... - Calling all western blot gurus! (reply: 3)

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