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Top : New Forum Archives (2009-): : Protein-and-Proteomics
661. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
662. fluorescent proteins expressed in nucleus - (reply: 6)
663. two bands detected in western - (reply: 1)
664. Ponceau S dilemma - "Trichloroacetic" or "Trifluoroacetic" acid (reply: 2)
665. Protein SDS-PAGE thick bands / smear - (reply: 4)
666. negative control in IP - (reply: 2)
667. Replacement of Zinc sulphate in Zinc staining - by using zinc chloride (reply: 2)
668. EMSA gels - (reply: 2)
669. gst pulldown - (reply: 2)
670. Co-IP - (reply: 2)
671. Across blot comparisons - (reply: 2)
672. Western blot data analysis - (reply: 1)
673. Native molecular weight determination - using restricted SDS in PAGE (reply: 2)
674. Lost protein after gel filtration - Gel Filtration (reply: 6)
675. Colorimetric kinase assay - (reply: 2)
676. nuclear extraction problem - nuclei gets too clumpy - (reply: 3)
677. protein size - (reply: 1)
678. is my lost protein is bcoz of cell death or my treatment worked??? - (reply: 2)
679. immunoprecipitation - immunoprecipitation help needed (reply: 1)
680. estimate size of protein - (reply: 2)
681. Problem- wierd 2D gel image - (reply: 5)
682. Protein elution from GST-fusion protein complex - (reply: 3)
683. total cell protein vs membrane protein - (reply: 4)
684. gst fusion protein - (reply: 3)
685. how to extract enzyme allinase from garlic - (reply: 1)
686. Quantitative Western Imaging - (reply: 3)
687. What causes this offset band on RIPA gel? - (reply: 4)
688. What portion of protein can I get under this protocol? - (reply: 3)
689. Squished lanes in SDS Page - (reply: 4)
690. Protin helicase (urgent) - helicase activity after long term storage (reply: 3)