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Top : New Forum Archives (2009-): : Protein-and-Proteomics
661. What causes this offset band on RIPA gel? - (reply: 4)
662. What portion of protein can I get under this protocol? - (reply: 3)
663. Squished lanes in SDS Page - (reply: 4)
664. Protin helicase (urgent) - helicase activity after long term storage (reply: 3)
665. BN-PAGE - (reply: 2)
666. How can I get protein in soluble fration - (reply: 2)
667. How long does it takes to make a crystallography of a protein? - (reply: 1)
668. how to detect a knowen protein's receptor? - (reply: 1)
669. protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
670. western blot to a metal mesh - (reply: 3)
671. Protein quantification - (reply: 2)
672. Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
673. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
674. Custom Primary Antibody - (reply: 3)
675. substrate for kinase - finding the predicted substrate for a kinase (reply: 6)
676. when should I add IPTG? - (reply: 2)
677. No protein spots in 2D gel - Silver staining! - (reply: 17)
678. appropriate time for induction (adding IPTG) - (reply: 1)
679. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
680. protein crystallography services question - (reply: 1)
681. Probelm with gel filtration - (reply: 4)
682. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
683. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
684. best and simple method to purify protein - (reply: 38)
685. Pipet trypsin tips - (reply: 3)
686. Are proteases a problem in protein quantification assays? - (reply: 3)
687. serum housekeeping proteins for total protein normalization - (reply: 1)
688. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
689. Co-IP on nuclear fraction - (reply: 2)
690. Problem with dot blot - Denatured sample??? (reply: 1)

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