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Top : New Forum Archives (2009-): : Protein-and-Proteomics
61. Bradford assay - (reply: 2)
62. SDS and urea lysis, protein degradation and freeze-thaw cycles - (reply: 4)
63. Suggestion for anti CD44 antibody for WB and IP? - (reply: 1)
64. Thrombin inactivation - (reply: 2)
65. IP - crosslinking antibody to dynabeads vs. NHS-acitvated beads? - (reply: 2)
66. Can anyone give a protocol to screen levels of adenosine deaminase in serum samp - (reply: 2)
67. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ri - (reply: 1)
68. half life of a protein - (reply: 3)
69. Western blot using blood plasma - (reply: 5)
70. PelB Leader Sequence Cleavage - (reply: 1)
71. Probing protein - (reply: 1)
72. production of phosphorylated proteins in E.coli host system - (reply: 1)
73. What is the reason of doing proteomics work? - (reply: 2)
74. Protein quantification - (reply: 4)
75. Protein extraction for 2D electrophoresis - (reply: 2)
76. Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
77. Test for protein in lysate - (reply: 4)
78. co-IP stained with coomassie blue - (reply: 2)
79. Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
80. How many PBMCs should I seed so that I can get enough protein? - (reply: 1)
81. co-ip of endogenous and tagged proteins - (reply: 1)
82. Histone extraction from S.cerevisiae - (reply: 1)
83. coomassie blue stain - (reply: 1)
84. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
85. concentation calculations - (reply: 1)
86. Vesicular Monoamine Transporter2 (VMAT2) - (reply: 8)
87. Protein sequence conservation - (reply: 2)
88. MALDI MS resutls to identify proteins - (reply: 2)
89. Dye with thiol group - (reply: 1)
90. CoIP - (reply: 2)

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