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61. How to elute antigen during immunoprecipitation experiment with out primary anti - (reply: 1)
62. dialyse of a protein - (reply: 4)
63. Western Blot, Bax, Many bands - (reply: 3)
64. Bradford assay Standard Curve Calculation - (reply: 3)
65. Protein Extraction: Extremely Sticky e coli lysate - (reply: 1)
66. Mass-spec compatible silver stain - (reply: 2)
67. I forgot to put ice block in transfer buffer, does it matter? - (reply: 7)
68. Problem with subcellular fractionation - (reply: 1)
69. How to stabilized a recombinant protein - (reply: 1)
70. Total protein determination before Elisa? - (reply: 2)
71. Mass-spectrometry of membrane proteins - (reply: 1)
72. phage lysate purification - (reply: 2)
73. CCRK (CDK20) protein expression and western blot - (reply: 7)
74. Enzyme Kinetics - (reply: 2)
75. Histone extraction from brain tissue - (reply: 1)
76. IGF2 antibody for immunohistochemistry/WB in mouse? - (reply: 2)
77. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
78. TFE in inducing alpha-helix formation in proteins - (reply: 3)
79. immunoprecipitation troubleshooting - (reply: 1)
80. Acetone Precipitation of proteins - (reply: 1)
81. Thrombin - how to use it in the best way? - (reply: 2)
82. dephosphorylate serines - (reply: 1)
83. rpHPLC chromatograph shift - (reply: 3)
84. Kinase pulldown - (reply: 1)
85. protein not stained by Coomassie Blue - (reply: 13)
86. Protein stability at pH8.3 - (reply: 2)
87. Software Suggestions - Short Peptides - (reply: 3)
88. Purification of FC tagged proteins - (reply: 3)
89. Most annoying Co-IP ever! Halp! - (reply: 2)
90. Vesicle immunoprecipitation and Blue Native PAGE - (reply: 1)
62. dialyse of a protein - (reply: 4)
63. Western Blot, Bax, Many bands - (reply: 3)
64. Bradford assay Standard Curve Calculation - (reply: 3)
65. Protein Extraction: Extremely Sticky e coli lysate - (reply: 1)
66. Mass-spec compatible silver stain - (reply: 2)
67. I forgot to put ice block in transfer buffer, does it matter? - (reply: 7)
68. Problem with subcellular fractionation - (reply: 1)
69. How to stabilized a recombinant protein - (reply: 1)
70. Total protein determination before Elisa? - (reply: 2)
71. Mass-spectrometry of membrane proteins - (reply: 1)
72. phage lysate purification - (reply: 2)
73. CCRK (CDK20) protein expression and western blot - (reply: 7)
74. Enzyme Kinetics - (reply: 2)
75. Histone extraction from brain tissue - (reply: 1)
76. IGF2 antibody for immunohistochemistry/WB in mouse? - (reply: 2)
77. myc-tag antibody makes multiple bands, even in mock & parental cells - (reply: 1)
78. TFE in inducing alpha-helix formation in proteins - (reply: 3)
79. immunoprecipitation troubleshooting - (reply: 1)
80. Acetone Precipitation of proteins - (reply: 1)
81. Thrombin - how to use it in the best way? - (reply: 2)
82. dephosphorylate serines - (reply: 1)
83. rpHPLC chromatograph shift - (reply: 3)
84. Kinase pulldown - (reply: 1)
85. protein not stained by Coomassie Blue - (reply: 13)
86. Protein stability at pH8.3 - (reply: 2)
87. Software Suggestions - Short Peptides - (reply: 3)
88. Purification of FC tagged proteins - (reply: 3)
89. Most annoying Co-IP ever! Halp! - (reply: 2)
90. Vesicle immunoprecipitation and Blue Native PAGE - (reply: 1)