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Top : New Forum Archives (2009-): : Protein-and-Proteomics
301. HIS elution: can imidazole be added after SDS-sample buffer? - (reply: 1)
302. What is Pellet that forms AFTER quick spin of boiled lysates? - (reply: 1)
303. ethanol precipitation of protein - (reply: 1)
304. How to thoroughly solubilize proteins from tissue sample, for quantitation - (reply: 1)
305. Protein Concentration from Bacterial Lysates: Help! - (reply: 2)
306. Is DTT reduction and alkylation critical before concentrating proteins? - (reply: 1)
307. Tricine SDS-PAGE - (reply: 3)
308. Pichia growth in MGY/MGYH media - (reply: 1)
309. protien purification using flag affinity - (reply: 4)
310. Constitutive p42/44 MAPK phosphorylation - (reply: 2)
311. His Tag Cleavage (protein precipitates) - (reply: 3)
312. Lysis buffer for Co-IP - (reply: 2)
313. how to get rid of post-translational modifications - (reply: 4)
314. HiTrap Heparin HP for purification of DNA binding proteins. - (reply: 1)
315. Exact molecular weight? - (reply: 1)
316. SDS-PAGE w/ PFA fixed tissue - (reply: 4)
317. How exactly do i show two proteins interacting? - (reply: 2)
318. AKTA/Unicorn compatibility with Windows 7? - (reply: 4)
319. band shift after IP - (reply: 1)
320. All-encompassing proteomics workflow? - (reply: 1)
321. staining artifacts in polyacrylamide gel ?? - (reply: 2)
322. What's so challenging about metablomics? - (reply: 1)
323. Ingineered di-sulfide bond - how-to? - (reply: 1)
324. Freezing cell lysates for westerns - (reply: 2)
325. problem with purification of gst-tagged protein - (reply: 2)
326. Collagen control in SDS-PAGE - (reply: 6)
327. problem with protein identification - (reply: 8)
328. F(abí)2 Preparation method?? - (reply: 1)
329. sf9 Insect cells questions - (reply: 3)
330. What is the yeild expected for an efficient IP? - (reply: 2)