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Top : New Forum Archives (2009-): : Protein-and-Proteomics
301. Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
302. Far western blotting - (reply: 5)
303. citrate buffer pH 8 - (reply: 1)
304. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
305. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
306. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
307. getting rid of non specific bands in IP experiments - (reply: 7)
308. loading control antibodies - (reply: 1)
309. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
310. detection of phosphorylation from cell pellet - (reply: 2)
311. how to make a recombinant enzyme refolded - (reply: 2)
312. Quantification of LDL Oxidation - (reply: 1)
313. Concentrating protein of a particular size - (reply: 1)
314. Removing a binding site - (reply: 5)
315. GFP 96-well black plates; plastic lid, sealer or paraffin oil to cover? - (reply: 3)
316. 96 well protein expression - (reply: 1)
317. IEF migration to middle of gel, no separation - (reply: 2)
318. P450 Activity Assay - (reply: 1)
319. Help with iTRAQ-SCX protocol - (reply: 2)
320. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
321. Problem: peptide (MW 2.3KDa) can't dialyze out - (reply: 5)
322. Anti-his antibodies - Noob to protein work - (reply: 10)
323. predict possible protein protein interaction - (reply: 1)
324. Western Blotting - (reply: 10)
325. Reuse of 2-Mercaptoethanol added buffer - (reply: 1)
326. how to make nondenaturing gradient gel - (reply: 2)
327. brain and colon tissue homogenate for WB - (reply: 3)
328. Detection of glycosylations on Western blotted proteins - (reply: 2)
329. Massive contaminant with TCA/acetone precipitation - (reply: 2)
330. western blot loading control - (reply: 2)

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