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Top : New Forum Archives (2009-): : Protein-and-Proteomics
301. sample preparation from whole cell lysates for Edman degradation - (reply: 2)
302. Role of Isoleucine in protein structure. - (reply: 1)
303. co-IP after ubiquitin cleavage - (reply: 5)
304. Heterologous gene expression in yeast - a bioinformatician needs your help cloni - (reply: 1)
305. Peptide Conc @215 and 225nm? - (reply: 1)
306. Insoluble material floats over my SDS lysate of human cells after centrifugation - (reply: 7)
307. Detection method of insulin level from blood serum - (reply: 2)
308. Relationship between total protein and enzyme activity - (reply: 3)
309. about cross link the antibody to ProteinG - (reply: 1)
310. EMSA supershift problem - (reply: 1)
311. Co-IP: Prey Protein Present in Neg and Pos Eluates - (reply: 5)
312. covalent-bound HIS complex can not be pull down: help needed - (reply: 2)
313. Protein extraction from mouse tissue? - (reply: 1)
314. Including "M" and "STOP" codons in my insert - (reply: 2)
315. Coomassie stain forzen and thawed gel? - (reply: 1)
316. IEF gel detaching during run - (reply: 7)
317. Overexpressed protein shows a band higher than expected?! - (reply: 2)
318. Bradford Protein Assay - Problem in BSA Standard Curve - (reply: 2)
319. HIS elution: can imidazole be added after SDS-sample buffer? - (reply: 1)
320. What is Pellet that forms AFTER quick spin of boiled lysates? - (reply: 1)
321. ethanol precipitation of protein - (reply: 1)
322. How to thoroughly solubilize proteins from tissue sample, for quantitation - (reply: 1)
323. Protein Concentration from Bacterial Lysates: Help! - (reply: 2)
324. Is DTT reduction and alkylation critical before concentrating proteins? - (reply: 1)
325. Tricine SDS-PAGE - (reply: 3)
326. Pichia growth in MGY/MGYH media - (reply: 1)
327. protien purification using flag affinity - (reply: 4)
328. Constitutive p42/44 MAPK phosphorylation - (reply: 2)
329. His Tag Cleavage (protein precipitates) - (reply: 3)
330. Lysis buffer for Co-IP - (reply: 2)