181. predict possible protein protein interaction - (reply: 1)
182. Western Blotting - (reply: 10)
183. Reuse of 2-Mercaptoethanol added buffer - (reply: 1)
184. how to make nondenaturing gradient gel - (reply: 2)
185. brain and colon tissue homogenate for WB - (reply: 3)
186. Detection of glycosylations on Western blotted proteins - (reply: 2)
187. Massive contaminant with TCA/acetone precipitation - (reply: 2)
188. western blot loading control - (reply: 2)
189. Insulin receptor mobility - (reply: 1)
190. lysis buffer not lysing adherent cells? - (reply: 8)
191. Properties of amino acids and protein functional activity - (reply: 1)
192. Co-IP and LC-Mass - (reply: 2)
193. Phosphorylation - (reply: 2)
194. Protein tagging with GFP/effect of expression construct - (reply: 2)
195. Analyzing Western Bands by Densiometry - (reply: 2)
196. Molecular weights of Insulin A chain and B chain? - (reply: 2)
197. Signaling pathways - minutes or hours? - (reply: 2)
198. what is the molecular weight of fusion protein in pET-32a - molecular weight of - (reply: 1)
199. Peptide Protein Conjugation - (reply: 2)
200. meaningless protein-protein interaction exists? - (reply: 2)
201. Kinase assay problem - (reply: 2)
202. TCA/acetone precipitation - no visible pellet for 500 micrograms ! - (reply: 3)
203. Ultrasonic bath for cell lysis - (reply: 3)
204. dot blot-protoplasts - (reply: 1)
205. Storage of BSA Standards - (reply: 7)
206. Optimising recombinant protein expression level in Saccharomyces cerevisiae - (reply: 1)
207. buffer exchange - (reply: 3)
208. nuclear extraction question - (reply: 6)
209. How many cells needed for co-IP and mass spec - (reply: 4)
210. Cell lysate turbidity upon long storage - (reply: 3)

© 1999-2010 Protocol Online, All rights reserved.