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Top : New Forum Archives (2009-): : Protein-and-Proteomics
571. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
572. Protein expression declines after overnight incubation - (reply: 4)
573. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
574. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
575. Fusion protein linker - (reply: 2)
576. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
577. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
578. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
579. Protein streaking on Blue Native-PAGE - (reply: 9)
580. basic questions about protein assays and cuvettes - (reply: 4)
581. How to attain pure chains of one protein complex - (reply: 1)
582. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
583. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
584. Fusion Protein - (reply: 3)
585. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
586. Need to find a method of detection for free Histag - (reply: 1)
587. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
588. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
589. High mRNA but no protein expression - (reply: 10)
590. Running a western with cell media in gels? - (reply: 11)
591. Transfer buffer as running buffer? - (reply: 7)
592. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
593. high background after ECL exposition - (reply: 3)
594. Circular Dichroism and disordered proteins - (reply: 1)
595. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
596. inactive enzyme from purification - (reply: 1)
597. Please comment on this Co-ip method - (reply: 2)
598. protein phosphorylation - methods for protein phosphorylation (reply: 9)
599. Co-IP of secreted proteins - (reply: 1)
600. Are protease inhibitors overrated ? - (reply: 2)