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Top : New Forum Archives (2009-): : Protein-and-Proteomics
571. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
572. protein prep - about prptein preperation in diagnosis (reply: 1)
573. blocking for 48hours - (reply: 1)
574. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
575. SDS PAGE - Bubble inside the Gel (reply: 5)
576. loss of protein during concentration - (reply: 6)
577. protease inhibitors and bradford - (reply: 2)
578. Cell lysates - Protein precipitation? - (reply: 3)
579. Which lysis buffer should I use? - (reply: 5)
580. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
581. cell lysate for pulldown - (reply: 3)
582. two hybrid system - opinion (reply: 1)
583. How many kDa add a post-translation modification? - (reply: 2)
584. making gel in advance - to save time (reply: 7)
585. peptide markers for native PAGE - (reply: 3)
586. protein sequencing company - (reply: 2)
587. Protein extraction from mouse prostate - (reply: 3)
588. Immunoprecipitation Troubleshooting - Immunoprecipitation (reply: 7)
589. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
590. fluorescent proteins expressed in nucleus - (reply: 6)
591. two bands detected in western - (reply: 1)
592. Ponceau S dilemma - "Trichloroacetic" or "Trifluoroacetic" acid (reply: 2)
593. Protein SDS-PAGE thick bands / smear - (reply: 4)
594. negative control in IP - (reply: 2)
595. Replacement of Zinc sulphate in Zinc staining - by using zinc chloride (reply: 2)
596. EMSA gels - (reply: 2)
597. gst pulldown - (reply: 2)
598. Co-IP - (reply: 2)
599. Across blot comparisons - (reply: 2)
600. Western blot data analysis - (reply: 1)

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