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Top : New Forum Archives (2009-): : Protein-and-Proteomics
571. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
572. Protein kinase protocols - (reply: 2)
573. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
574. Purification of His tagged protein - (reply: 2)
575. Protease and proteasome inhibitors - (reply: 2)
576. BCA diluent and standard curve - (reply: 1)
577. protein induction problem - (reply: 4)
578. Growing insect cells with other cell lines - (reply: 2)
579. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
580. Protein expression declines after overnight incubation - (reply: 4)
581. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
582. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
583. Fusion protein linker - (reply: 2)
584. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
585. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
586. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
587. Protein streaking on Blue Native-PAGE - (reply: 9)
588. basic questions about protein assays and cuvettes - (reply: 4)
589. How to attain pure chains of one protein complex - (reply: 1)
590. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
591. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
592. Fusion Protein - (reply: 3)
593. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
594. Need to find a method of detection for free Histag - (reply: 1)
595. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
596. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
597. High mRNA but no protein expression - (reply: 10)
598. Running a western with cell media in gels? - (reply: 11)
599. Transfer buffer as running buffer? - (reply: 7)
600. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)