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Top : New Forum Archives (2009-): : Protein-and-Proteomics
751. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
752. EMSA probes - method to make longer probes (reply: 3)
753. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
754. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
755. GST pull down - (reply: 1)
756. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
757. Determining how much enzyme to use based on activity units - (reply: 3)
758. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
759. yield vs concentration of protein - (reply: 3)
760. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
761. Sonication mysteries - (reply: 4)
762. cys rich protein expression - (reply: 1)
763. immunoprecipitation troubleshooting - (reply: 1)
764. Immunoprecipitation with two antibodies - (reply: 10)
765. RIPA Buffer precipitation trouble - (reply: 5)
766. develop western by ECL plus - (reply: 1)
767. Protein extraction from tissue - (reply: 1)
768. problem of membrane protein FPLC - (reply: 1)
769. spot removal from 2D gel and proteomics - help! (reply: 1)
770. Co-IP problem - (reply: 3)
771. I am confused, please help. - (reply: 1)
772. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
773. EMSA using biotin labeled probes and buffers - (reply: 5)
774. Foam in the BSA solution - (reply: 3)
775. Inclusion Body purification - (reply: 2)
776. How to design an antibody - (reply: 1)
777. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
778. Protein isolation from skin - (reply: 2)
779. How to concentrate my protein? - (reply: 1)
780. Organism specific protein?? - data from maldi-ms (reply: 6)

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