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1141. help! Truncated protein cannot expressed in mammalian cells? - (reply: 6)
1142. starry sky western blot... lots of spots. - (reply: 5)
1143. IEF buffer (cathod and anode) for range 4-6 - (reply: 3)
1144. blood contamination - (reply: 3)
1145. Crystallization - (reply: 2)
1146. in vivo sumoylation - (reply: 4)
1147. Solubaility Curve - (reply: 1)
1148. How much R^2 that can be accept for standard curve of Bradford's assay? - (reply: 4)
1149. Signal peptide - (reply: 1)
1150. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
1151. IP Problems - Cant detect antigen PLEASE HELP! (reply: 2)
1152. Antibody storage - (reply: 10)
1153. lysis buffer to use for enzyme assay - (reply: 1)
1154. Enzyme IU - (reply: 1)
1155. gel shift in western blot - (reply: 3)
1156. can beta-catenin be used as a house keeping protein? - (reply: 3)
1157. PMSF - (reply: 2)
1158. WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
1159. Mass Selection in Mass Spectrometry - (reply: 3)
1160. Semidry Transfer - (reply: 4)
1161. IP problem: antibody binds too few antigen - (reply: 13)
1162. Bubble test - (reply: 7)
1163. HPLC baseline problem - (reply: 1)
1164. Detergent concentration for nuclear membrane dissolving - (reply: 2)
1165. Instant Blue / Stain - any recipes? (reply: 3)
1166. Tissue sonication for RNA extraction - Help :) (reply: 1)
1167. protein precipitation - (reply: 1)
1168. brown pigment attached to the protein - (reply: 3)
1169. std for bradford assay - (reply: 6)
1170. Is anyone using iTRAQ? - how do you deal with sample complexity? (reply: 2)
1142. starry sky western blot... lots of spots. - (reply: 5)
1143. IEF buffer (cathod and anode) for range 4-6 - (reply: 3)
1144. blood contamination - (reply: 3)
1145. Crystallization - (reply: 2)
1146. in vivo sumoylation - (reply: 4)
1147. Solubaility Curve - (reply: 1)
1148. How much R^2 that can be accept for standard curve of Bradford's assay? - (reply: 4)
1149. Signal peptide - (reply: 1)
1150. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
1151. IP Problems - Cant detect antigen PLEASE HELP! (reply: 2)
1152. Antibody storage - (reply: 10)
1153. lysis buffer to use for enzyme assay - (reply: 1)
1154. Enzyme IU - (reply: 1)
1155. gel shift in western blot - (reply: 3)
1156. can beta-catenin be used as a house keeping protein? - (reply: 3)
1157. PMSF - (reply: 2)
1158. WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
1159. Mass Selection in Mass Spectrometry - (reply: 3)
1160. Semidry Transfer - (reply: 4)
1161. IP problem: antibody binds too few antigen - (reply: 13)
1162. Bubble test - (reply: 7)
1163. HPLC baseline problem - (reply: 1)
1164. Detergent concentration for nuclear membrane dissolving - (reply: 2)
1165. Instant Blue / Stain - any recipes? (reply: 3)
1166. Tissue sonication for RNA extraction - Help :) (reply: 1)
1167. protein precipitation - (reply: 1)
1168. brown pigment attached to the protein - (reply: 3)
1169. std for bradford assay - (reply: 6)
1170. Is anyone using iTRAQ? - how do you deal with sample complexity? (reply: 2)