Protocol Online logo
Top : New Forum Archives (2009-): : Protein-and-Proteomics
1141. in vivo sumoylation - (reply: 4)
1142. Solubaility Curve - (reply: 1)
1143. How much R^2 that can be accept for standard curve of Bradford's assay? - (reply: 4)
1144. Signal peptide - (reply: 1)
1145. How to purify a protein (PI 8.0) using His tag? - (reply: 4)
1146. IP Problems - Cant detect antigen PLEASE HELP! (reply: 2)
1147. Antibody storage - (reply: 10)
1148. lysis buffer to use for enzyme assay - (reply: 1)
1149. Enzyme IU - (reply: 1)
1150. gel shift in western blot - (reply: 3)
1151. can beta-catenin be used as a house keeping protein? - (reply: 3)
1152. PMSF - (reply: 2)
1153. WB for phosphorylated protein complex in rat intestinal tissue - (reply: 2)
1154. Mass Selection in Mass Spectrometry - (reply: 3)
1155. Semidry Transfer - (reply: 4)
1156. IP problem: antibody binds too few antigen - (reply: 13)
1157. Bubble test - (reply: 7)
1158. HPLC baseline problem - (reply: 1)
1159. Detergent concentration for nuclear membrane dissolving - (reply: 2)
1160. Instant Blue / Stain - any recipes? (reply: 3)
1161. Tissue sonication for RNA extraction - Help :) (reply: 1)
1162. protein precipitation - (reply: 1)
1163. brown pigment attached to the protein - (reply: 3)
1164. std for bradford assay - (reply: 6)
1165. Is anyone using iTRAQ? - how do you deal with sample complexity? (reply: 2)
1166. Protein extraction from mouse organs - (reply: 6)
1167. Immunoprecipitation (optimization?) problem - anti-ovalbumin lane has 225 kDa band. (reply: 7)
1168. part of protein sequence missing - (reply: 4)
1169. CO-IP problem: non specific prot binding to G beads - (reply: 11)
1170. Immunoprecipitation vs conventional WB - what are the differences? (reply: 7)