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511. Problem with dot blot - Denatured sample??? (reply: 1)
512. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
513. Odyssey Imaging and EMSA - (reply: 2)
514. 2d page - no band (reply: 2)
515. preparing immunoprecipitated sample for mass spec - (reply: 2)
516. Does Change pH could affect protein interaction - GST pul down (reply: 2)
517. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
518. ELISA peptide coating problem =( - I don’t know what to do to bound the peptide to the plates. (reply: 7)
519. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
520. Proteasome assay - (reply: 6)
521. protein extraction - ripa buffer vs urea buffer (reply: 1)
522. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
523. EMSA - probe design and competition - (reply: 6)
524. Internal control - (reply: 1)
525. IP my protein from culture supernatant - (reply: 2)
526. High pI proteins - High pI proteins (reply: 4)
527. antibody purification - ammonium sulfate precipitation - (reply: 7)
528. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
529. Protein Degradation after overexpression - (reply: 1)
530. Southern blot troubleshoot - (reply: 8)
531. IP from nuclear extract - (reply: 3)
532. Circular Dichroism - Can I use NaCl? (reply: 2)
533. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
534. Problems in upscaling protein expression - (reply: 2)
535. amonium precipitation - (reply: 3)
536. preserving bovine serum albumin with sodium azide - (reply: 3)
537. Where is the future of the proteomics? - (reply: 5)
538. BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)
539. Tris acetate sds buffer - (reply: 2)
540. Protein stabilization buffer needed - (reply: 1)
512. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
513. Odyssey Imaging and EMSA - (reply: 2)
514. 2d page - no band (reply: 2)
515. preparing immunoprecipitated sample for mass spec - (reply: 2)
516. Does Change pH could affect protein interaction - GST pul down (reply: 2)
517. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
518. ELISA peptide coating problem =( - I don’t know what to do to bound the peptide to the plates. (reply: 7)
519. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
520. Proteasome assay - (reply: 6)
521. protein extraction - ripa buffer vs urea buffer (reply: 1)
522. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
523. EMSA - probe design and competition - (reply: 6)
524. Internal control - (reply: 1)
525. IP my protein from culture supernatant - (reply: 2)
526. High pI proteins - High pI proteins (reply: 4)
527. antibody purification - ammonium sulfate precipitation - (reply: 7)
528. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
529. Protein Degradation after overexpression - (reply: 1)
530. Southern blot troubleshoot - (reply: 8)
531. IP from nuclear extract - (reply: 3)
532. Circular Dichroism - Can I use NaCl? (reply: 2)
533. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)
534. Problems in upscaling protein expression - (reply: 2)
535. amonium precipitation - (reply: 3)
536. preserving bovine serum albumin with sodium azide - (reply: 3)
537. Where is the future of the proteomics? - (reply: 5)
538. BSA Standard Curve Dilution (BCA Kit) - how to dilute the correct way? (reply: 6)
539. Tris acetate sds buffer - (reply: 2)
540. Protein stabilization buffer needed - (reply: 1)