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Top : New Forum Archives (2009-): : Protein-and-Proteomics
511. Protease and proteasome inhibitors - (reply: 2)
512. BCA diluent and standard curve - (reply: 1)
513. protein induction problem - (reply: 4)
514. Growing insect cells with other cell lines - (reply: 2)
515. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
516. Protein expression declines after overnight incubation - (reply: 4)
517. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
518. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
519. Fusion protein linker - (reply: 2)
520. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
521. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
522. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
523. Protein streaking on Blue Native-PAGE - (reply: 9)
524. basic questions about protein assays and cuvettes - (reply: 4)
525. How to attain pure chains of one protein complex - (reply: 1)
526. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
527. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
528. Fusion Protein - (reply: 3)
529. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
530. Need to find a method of detection for free Histag - (reply: 1)
531. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
532. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
533. High mRNA but no protein expression - (reply: 10)
534. Running a western with cell media in gels? - (reply: 11)
535. Transfer buffer as running buffer? - (reply: 7)
536. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
537. high background after ECL exposition - (reply: 3)
538. Circular Dichroism and disordered proteins - (reply: 1)
539. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
540. inactive enzyme from purification - (reply: 1)

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