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Top : New Forum Archives (2009-): : Protein-and-Proteomics
511. Protein isolation in zebrafish - (reply: 1)
512. Acetone washes for TCA precipitation protocoles - (reply: 3)
513. Biotinylation of protein in tris buffer - (reply: 2)
514. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
515. Protein kinase protocols - (reply: 2)
516. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
517. Purification of His tagged protein - (reply: 2)
518. Protease and proteasome inhibitors - (reply: 2)
519. BCA diluent and standard curve - (reply: 1)
520. protein induction problem - (reply: 4)
521. Growing insect cells with other cell lines - (reply: 2)
522. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
523. Protein expression declines after overnight incubation - (reply: 4)
524. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
525. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
526. Fusion protein linker - (reply: 2)
527. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
528. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
529. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
530. Protein streaking on Blue Native-PAGE - (reply: 9)
531. basic questions about protein assays and cuvettes - (reply: 4)
532. How to attain pure chains of one protein complex - (reply: 1)
533. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
534. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
535. Fusion Protein - (reply: 3)
536. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
537. Need to find a method of detection for free Histag - (reply: 1)
538. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
539. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
540. High mRNA but no protein expression - (reply: 10)

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