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Top : New Forum Archives (2009-): : Protein-and-Proteomics
511. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
512. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
513. High mRNA but no protein expression - (reply: 10)
514. Running a western with cell media in gels? - (reply: 11)
515. Transfer buffer as running buffer? - (reply: 7)
516. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
517. high background after ECL exposition - (reply: 3)
518. Circular Dichroism and disordered proteins - (reply: 1)
519. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
520. inactive enzyme from purification - (reply: 1)
521. Please comment on this Co-ip method - (reply: 2)
522. protein phosphorylation - methods for protein phosphorylation (reply: 9)
523. Co-IP of secreted proteins - (reply: 1)
524. Are protease inhibitors overrated ? - (reply: 2)
525. gst- pulldown - (reply: 1)
526. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
527. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
528. 143 kDa protein - (reply: 3)
529. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
530. Purification od insoluble protein (TIMP3) - (reply: 2)
531. descrepancy between total cell lysate and cell fractionation - (reply: 1)
532. Immunopricipitation for lysate only? - (reply: 3)
533. eluting gst fusion proteins - (reply: 1)
534. How to pull down chromatin and identify protein associated with it? - (reply: 1)
535. expression at 30 C - (reply: 1)
536. Protein extraction from perfusion fixed brain tissue - (reply: 5)
537. GST pulldown: elude or not? - (reply: 1)
538. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
539. secreted protein quantification - (reply: 2)
540. Am. Sulp Precipitation - (reply: 2)

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