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Top : New Forum Archives (2009-): : Protein-and-Proteomics
511. His-tag protein vs. BSA - (reply: 3)
512. Chromatography column packing - (reply: 2)
513. Almost always NaCl in buffers - Why? - NaCl seems to be the gold standard in buffers - why? (reply: 3)
514. Biotin - BMCC - (reply: 1)
515. Strange results in biotin-avidin pulldown - has anyone ever got it like that?????? (reply: 3)
516. Unstable expression in BL21 DE3 strain - (reply: 4)
517. YFP protein fusion accumulates - (reply: 10)
518. Dry transfer - (reply: 8)
519. 4-MUG stability - in -20 over 15 years (reply: 1)
520. One Shot TOP10 Cells - (reply: 6)
521. problem of 2D-GE with phagosome fractions - (reply: 7)
522. Blocking beads for immunoprecipitation - (reply: 1)
523. Lactobacillus crude protein extraction? - (reply: 8)
524. Size of Prostaglandin, who knows? - (reply: 1)
525. Fusion tagged protein and Western Blot problem - (reply: 3)
526. Compatible detergents for proteomics - (reply: 4)
527. Immunoprecipitation problem - Protein nonspecific binding to bead (reply: 2)
528. Troubleshooting Protein Purification using FPLC - (reply: 1)
529. Sodium Butyrate Stock Preparation - pH; histone preparation; acetylated histone (reply: 2)
530. Problem with NE-PER cytoplasmatic and nuclear separation kit - (reply: 1)
531. his- & biotin- tag protein purfication - (reply: 2)
532. How to remove protein to show an activity is protein based - (reply: 7)
533. Gel filtration Chromatography - (reply: 1)
534. Western: Mouse antibody on mouse tissue homogenates - (reply: 3)
535. Question about Urea - (reply: 2)
536. help with his tagged protein purification - precipitation problem (reply: 3)
537. Enzymes Technology - Enzymic Hydrolysis (reply: 1)
538. Stacking Gel Polymerization - (reply: 4)
539. Is it possible to see a dimer/oligomer in denaturing SDS-PAGE? - (reply: 1)
540. Quick disapperance of ECL signal - (reply: 1)