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511. RP-HPLC carryover problems - (reply: 1)
512. Boiling or shaking on ice? - (reply: 2)
513. Concentrated conditioned medium for western blot - (reply: 1)
514. phospho akt and akt - does p-akt antibody recognize normal akt? (reply: 3)
515. Purification of recombinant proteins from insect cells - (reply: 2)
516. similar size of proteins but different movement on SDS-PAGE - (reply: 1)
517. Protein Expression in yeast - protein not expressed (reply: 1)
518. cheap and fast enzyme activity assay? - (reply: 1)
519. Protein quantification after 8M urea lysis - (reply: 3)
520. phosphorylted protein smaller not bigger? - (reply: 1)
521. protein phosphorylation status in sample buffer - (reply: 2)
522. Different profile of the same sample in different IEC column - (reply: 5)
523. 2d gel technique - (reply: 2)
524. Boiling, protein aggregation......reversal?? (western) - (reply: 3)
525. insoluble protein expression help - (reply: 3)
526. Model Protein - Need Advice! - (reply: 3)
527. Co-IP, what should I get? - (reply: 1)
528. CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
529. His-tag protein vs. BSA - (reply: 3)
530. Chromatography column packing - (reply: 2)
531. Almost always NaCl in buffers - Why? - NaCl seems to be the gold standard in buffers - why? (reply: 3)
532. Biotin - BMCC - (reply: 1)
533. Strange results in biotin-avidin pulldown - has anyone ever got it like that?????? (reply: 3)
534. Unstable expression in BL21 DE3 strain - (reply: 4)
535. YFP protein fusion accumulates - (reply: 10)
536. Dry transfer - (reply: 8)
537. 4-MUG stability - in -20 over 15 years (reply: 1)
538. One Shot TOP10 Cells - (reply: 6)
539. problem of 2D-GE with phagosome fractions - (reply: 7)
540. Blocking beads for immunoprecipitation - (reply: 1)
512. Boiling or shaking on ice? - (reply: 2)
513. Concentrated conditioned medium for western blot - (reply: 1)
514. phospho akt and akt - does p-akt antibody recognize normal akt? (reply: 3)
515. Purification of recombinant proteins from insect cells - (reply: 2)
516. similar size of proteins but different movement on SDS-PAGE - (reply: 1)
517. Protein Expression in yeast - protein not expressed (reply: 1)
518. cheap and fast enzyme activity assay? - (reply: 1)
519. Protein quantification after 8M urea lysis - (reply: 3)
520. phosphorylted protein smaller not bigger? - (reply: 1)
521. protein phosphorylation status in sample buffer - (reply: 2)
522. Different profile of the same sample in different IEC column - (reply: 5)
523. 2d gel technique - (reply: 2)
524. Boiling, protein aggregation......reversal?? (western) - (reply: 3)
525. insoluble protein expression help - (reply: 3)
526. Model Protein - Need Advice! - (reply: 3)
527. Co-IP, what should I get? - (reply: 1)
528. CHOP protein western blot...help reqd! - transfer conditions for CHOP protein (reply: 3)
529. His-tag protein vs. BSA - (reply: 3)
530. Chromatography column packing - (reply: 2)
531. Almost always NaCl in buffers - Why? - NaCl seems to be the gold standard in buffers - why? (reply: 3)
532. Biotin - BMCC - (reply: 1)
533. Strange results in biotin-avidin pulldown - has anyone ever got it like that?????? (reply: 3)
534. Unstable expression in BL21 DE3 strain - (reply: 4)
535. YFP protein fusion accumulates - (reply: 10)
536. Dry transfer - (reply: 8)
537. 4-MUG stability - in -20 over 15 years (reply: 1)
538. One Shot TOP10 Cells - (reply: 6)
539. problem of 2D-GE with phagosome fractions - (reply: 7)
540. Blocking beads for immunoprecipitation - (reply: 1)