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Top : New Forum Archives (2009-): : Protein-and-Proteomics
511. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
512. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
513. Fusion Protein - (reply: 3)
514. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
515. Need to find a method of detection for free Histag - (reply: 1)
516. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
517. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
518. High mRNA but no protein expression - (reply: 10)
519. Running a western with cell media in gels? - (reply: 11)
520. Transfer buffer as running buffer? - (reply: 7)
521. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
522. high background after ECL exposition - (reply: 3)
523. Circular Dichroism and disordered proteins - (reply: 1)
524. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
525. inactive enzyme from purification - (reply: 1)
526. Please comment on this Co-ip method - (reply: 2)
527. protein phosphorylation - methods for protein phosphorylation (reply: 9)
528. Co-IP of secreted proteins - (reply: 1)
529. Are protease inhibitors overrated ? - (reply: 2)
530. gst- pulldown - (reply: 1)
531. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
532. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
533. 143 kDa protein - (reply: 3)
534. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
535. Purification od insoluble protein (TIMP3) - (reply: 2)
536. descrepancy between total cell lysate and cell fractionation - (reply: 1)
537. Immunopricipitation for lysate only? - (reply: 3)
538. eluting gst fusion proteins - (reply: 1)
539. How to pull down chromatin and identify protein associated with it? - (reply: 1)
540. expression at 30 C - (reply: 1)

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