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Top : New Forum Archives (2009-): : Protein-and-Proteomics
961. Protein synthesis inhibition - (reply: 4)
962. His tag protein purification - (reply: 3)
963. protein extraction from primary cells - (reply: 1)
964. Most strange sds page gel ever!!!!! - (reply: 5)
965. Minimal amounts of whole cell extracts for Co-IP - (reply: 1)
966. EDTA as denaturating agent ?? - for proteins.. (reply: 5)
967. GST pull down assay - (reply: 3)
968. Best yeast expression system - (reply: 1)
969. Help_Role of BSA for the protein reconstitution - (reply: 5)
970. BCA Protein Assay Problems - (reply: 1)
971. protein degardation during gel filteration - (reply: 2)
972. lysate fractionation techniques - (reply: 1)
973. Co-IP using magnetic beads, non-specific binding - (reply: 4)
974. Storage of 8M Urea buffer? - I made it two weeks ago? (reply: 3)
975. Protein solubility improvements? - any methods? (reply: 5)
976. SDS-DNA-PAGE - How to? (reply: 3)
977. antibody production..does it matter if the protein is denatured or not? - my fragments are insoluble :( (reply: 3)
978. Protein Smear in SDS-PAGE after concentrated? - (reply: 1)
979. western blotting loading control - (reply: 5)
980. How to do Co-IP of Triton x-100 insoluble proteins (e.g. intermediate filament p - (reply: 1)
981. Immunolotting of actin protein coming at higher MW - (reply: 1)
982. Isolation of secreted proteins from culture media - (reply: 2)
983. smear on gel after tca precipitation - (reply: 3)
984. Protein loading marker for glycoproteins - (reply: 2)
985. distinguish protein expression from high homology - (reply: 1)
986. (in vitro binding assay w/ IP)- a-Mouse Ab seems to recognize rabbit a-GFP Ab or - (reply: 2)
987. How to store membrane with primary antibody? - (reply: 5)
988. Protein Removal from cell lysate - leaving RNA intact! (reply: 1)
989. Weird BSA standard curve for Bradford assay - (reply: 9)
990. non-specific band persistent at 29kDa - western blot non-specific bands (reply: 2)

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