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Top : New Forum Archives (2009-): : Protein-and-Proteomics
541. why phosphorylated protein relative to total protein? - (reply: 2)
542. Protocol for refolding by dilution - (reply: 1)
543. Freezing cell pellets and minimizing lysis - (reply: 2)
544. insoluble fusion protein - (reply: 1)
545. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
546. ammonium precipitation of Proteins - (reply: 2)
547. how to make antigen by trypsin and papain digetion - plz teel me hoe to make antigen by trypsin & papain digetion (reply: 1)
548. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
549. cathode buffer for CN-PAGE - (reply: 1)
550. M9 medium??? - (reply: 1)
551. refrozen cell pellet - (reply: 1)
552. NuPAGE Gel get smaller after staining o/n - (reply: 1)
553. Single amino acid substitutions away from the active site causing disease. - (reply: 4)
554. how to express protein in soluble form - (reply: 1)
555. IP no target protein in elution - (reply: 1)
556. Protein stability - (reply: 4)
557. Relation between microns and kD - (reply: 3)
558. ATP assay - malachite green - (reply: 3)
559. PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
560. Protein isolation in zebrafish - (reply: 1)
561. Acetone washes for TCA precipitation protocoles - (reply: 3)
562. Biotinylation of protein in tris buffer - (reply: 2)
563. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
564. Protein kinase protocols - (reply: 2)
565. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
566. Purification of His tagged protein - (reply: 2)
567. Protease and proteasome inhibitors - (reply: 2)
568. BCA diluent and standard curve - (reply: 1)
569. protein induction problem - (reply: 4)
570. Growing insect cells with other cell lines - (reply: 2)