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Top : New Forum Archives (2009-): : Protein-and-Proteomics
541. Protein extraction from perfusion fixed brain tissue - (reply: 5)
542. GST pulldown: elude or not? - (reply: 1)
543. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
544. secreted protein quantification - (reply: 2)
545. Am. Sulp Precipitation - (reply: 2)
546. EMSA - Probe Annealing problems (reply: 1)
547. Mass spectrometry for dummies? - (reply: 3)
548. Major Protein Loss! Why? - (reply: 2)
549. Filming membrane (Western Blot & ECL) - (reply: 1)
550. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
551. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
552. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
553. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
554. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
555. protein purification - (reply: 5)
556. western blot is not consistent with immunofluorescence - (reply: 2)
557. determination of the Stoichiometry - (reply: 1)
558. cytoplasmic and nuclear extraction for BCA - (reply: 6)
559. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
560. protein prep - about prptein preperation in diagnosis (reply: 1)
561. blocking for 48hours - (reply: 1)
562. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
563. SDS PAGE - Bubble inside the Gel (reply: 5)
564. loss of protein during concentration - (reply: 6)
565. protease inhibitors and bradford - (reply: 2)
566. Cell lysates - Protein precipitation? - (reply: 3)
567. Which lysis buffer should I use? - (reply: 5)
568. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
569. cell lysate for pulldown - (reply: 3)
570. two hybrid system - opinion (reply: 1)

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