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Top : New Forum Archives (2009-): : Protein-and-Proteomics
541. Freezing cell pellets and minimizing lysis - (reply: 2)
542. insoluble fusion protein - (reply: 1)
543. Which wash buffer (to collect cell) is prefered for 2DE? - (reply: 3)
544. ammonium precipitation of Proteins - (reply: 2)
545. how to make antigen by trypsin and papain digetion - plz teel me hoe to make antigen by trypsin & papain digetion (reply: 1)
546. can i use trypsin to collect cell sample for 2d gel analysis? - (reply: 3)
547. cathode buffer for CN-PAGE - (reply: 1)
548. M9 medium??? - (reply: 1)
549. refrozen cell pellet - (reply: 1)
550. NuPAGE Gel get smaller after staining o/n - (reply: 1)
551. Single amino acid substitutions away from the active site causing disease. - (reply: 4)
552. how to express protein in soluble form - (reply: 1)
553. IP no target protein in elution - (reply: 1)
554. Protein stability - (reply: 4)
555. Relation between microns and kD - (reply: 3)
556. ATP assay - malachite green - (reply: 3)
557. PROTEIN QUANTIFICATION - cell lysis buffer (reply: 2)
558. Protein isolation in zebrafish - (reply: 1)
559. Acetone washes for TCA precipitation protocoles - (reply: 3)
560. Biotinylation of protein in tris buffer - (reply: 2)
561. HELP is needed for low expression using pEXP5-CT vector in BL21 (DE3) plyss - low expression after time course study with 1 mM IPTG (reply: 1)
562. Protein kinase protocols - (reply: 2)
563. Identification of Membrane Receptor protein, how ?? - I wanna use MS approach ! (reply: 4)
564. Purification of His tagged protein - (reply: 2)
565. Protease and proteasome inhibitors - (reply: 2)
566. BCA diluent and standard curve - (reply: 1)
567. protein induction problem - (reply: 4)
568. Growing insect cells with other cell lines - (reply: 2)
569. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
570. Protein expression declines after overnight incubation - (reply: 4)

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