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Top : New Forum Archives (2009-): : Protein-and-Proteomics
541. Fusion Protein - (reply: 3)
542. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
543. Need to find a method of detection for free Histag - (reply: 1)
544. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
545. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
546. High mRNA but no protein expression - (reply: 10)
547. Running a western with cell media in gels? - (reply: 11)
548. Transfer buffer as running buffer? - (reply: 7)
549. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
550. high background after ECL exposition - (reply: 3)
551. Circular Dichroism and disordered proteins - (reply: 1)
552. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
553. inactive enzyme from purification - (reply: 1)
554. Please comment on this Co-ip method - (reply: 2)
555. protein phosphorylation - methods for protein phosphorylation (reply: 9)
556. Co-IP of secreted proteins - (reply: 1)
557. Are protease inhibitors overrated ? - (reply: 2)
558. gst- pulldown - (reply: 1)
559. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
560. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
561. 143 kDa protein - (reply: 3)
562. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
563. Purification od insoluble protein (TIMP3) - (reply: 2)
564. descrepancy between total cell lysate and cell fractionation - (reply: 1)
565. Immunopricipitation for lysate only? - (reply: 3)
566. eluting gst fusion proteins - (reply: 1)
567. How to pull down chromatin and identify protein associated with it? - (reply: 1)
568. expression at 30 C - (reply: 1)
569. Protein extraction from perfusion fixed brain tissue - (reply: 5)
570. GST pulldown: elude or not? - (reply: 1)

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