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Top : New Forum Archives (2009-): : Protein-and-Proteomics
541. determination of the Stoichiometry - (reply: 1)
542. cytoplasmic and nuclear extraction for BCA - (reply: 6)
543. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
544. protein prep - about prptein preperation in diagnosis (reply: 1)
545. blocking for 48hours - (reply: 1)
546. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
547. SDS PAGE - Bubble inside the Gel (reply: 5)
548. loss of protein during concentration - (reply: 6)
549. protease inhibitors and bradford - (reply: 2)
550. Cell lysates - Protein precipitation? - (reply: 3)
551. Which lysis buffer should I use? - (reply: 5)
552. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
553. cell lysate for pulldown - (reply: 3)
554. two hybrid system - opinion (reply: 1)
555. How many kDa add a post-translation modification? - (reply: 2)
556. making gel in advance - to save time (reply: 7)
557. peptide markers for native PAGE - (reply: 3)
558. protein sequencing company - (reply: 2)
559. Protein extraction from mouse prostate - (reply: 3)
560. Immunoprecipitation Troubleshooting - Immunoprecipitation (reply: 7)
561. blocking the resin for his-tag pull down - what kind of blocking reagents (reply: 3)
562. fluorescent proteins expressed in nucleus - (reply: 6)
563. two bands detected in western - (reply: 1)
564. Ponceau S dilemma - "Trichloroacetic" or "Trifluoroacetic" acid (reply: 2)
565. Protein SDS-PAGE thick bands / smear - (reply: 4)
566. negative control in IP - (reply: 2)
567. Replacement of Zinc sulphate in Zinc staining - by using zinc chloride (reply: 2)
568. EMSA gels - (reply: 2)
569. gst pulldown - (reply: 2)
570. Co-IP - (reply: 2)

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