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151. How to transfer His-tag from N- to C-terminus in plasmid - (reply: 1)
152. GST tag protein- soluble or insoluble??? help! - (reply: 2)
153. Rapid western blot buffer ?? - (reply: 2)
154. How to get soluble protein? - (reply: 3)
155. 'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
156. Novel PTM site detection - (reply: 1)
157. Potassium phosphate buffer - (reply: 3)
158. Protein Isolation from mouse brain: problems - (reply: 2)
159. Expression level analysis at mRNA vs protein level - (reply: 6)
160. 【help】What could happen to this SDS-PAGE ? - (reply: 1)
161. Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
162. Far western blotting - (reply: 5)
163. citrate buffer pH 8 - (reply: 1)
164. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
165. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
166. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
167. getting rid of non specific bands in IP experiments - (reply: 7)
168. loading control antibodies - (reply: 1)
169. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
170. detection of phosphorylation from cell pellet - (reply: 2)
171. how to make a recombinant enzyme refolded - (reply: 2)
172. Quantification of LDL Oxidation - (reply: 1)
173. Concentrating protein of a particular size - (reply: 1)
174. Removing a binding site - (reply: 5)
175. GFP 96-well black plates; plastic lid, sealer or paraffin oil to cover? - (reply: 3)
176. 96 well protein expression - (reply: 1)
177. IEF migration to middle of gel, no separation - (reply: 2)
178. P450 Activity Assay - (reply: 1)
179. Help with iTRAQ-SCX protocol - (reply: 2)
180. 2-D electrophoresis of sperm membrane extracts - (reply: 9)
152. GST tag protein- soluble or insoluble??? help! - (reply: 2)
153. Rapid western blot buffer ?? - (reply: 2)
154. How to get soluble protein? - (reply: 3)
155. 'Standard salt buffer' for protein storage/biophysical techniques - (reply: 1)
156. Novel PTM site detection - (reply: 1)
157. Potassium phosphate buffer - (reply: 3)
158. Protein Isolation from mouse brain: problems - (reply: 2)
159. Expression level analysis at mRNA vs protein level - (reply: 6)
160. 【help】What could happen to this SDS-PAGE ? - (reply: 1)
161. Insoluble recombinant protein is expressed also with out IPTG - (reply: 4)
162. Far western blotting - (reply: 5)
163. citrate buffer pH 8 - (reply: 1)
164. What's the difference between megnatic beads and agarose beads to IP His-tag - (reply: 3)
165. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
166. Problem with removing fusion protein. MALDI TOF. Tricine SDS PAGE. - (reply: 1)
167. getting rid of non specific bands in IP experiments - (reply: 7)
168. loading control antibodies - (reply: 1)
169. protein solubilization and quantitation after extraction using Trizol - (reply: 2)
170. detection of phosphorylation from cell pellet - (reply: 2)
171. how to make a recombinant enzyme refolded - (reply: 2)
172. Quantification of LDL Oxidation - (reply: 1)
173. Concentrating protein of a particular size - (reply: 1)
174. Removing a binding site - (reply: 5)
175. GFP 96-well black plates; plastic lid, sealer or paraffin oil to cover? - (reply: 3)
176. 96 well protein expression - (reply: 1)
177. IEF migration to middle of gel, no separation - (reply: 2)
178. P450 Activity Assay - (reply: 1)
179. Help with iTRAQ-SCX protocol - (reply: 2)
180. 2-D electrophoresis of sperm membrane extracts - (reply: 9)