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Top : New Forum Archives (2009-): : Protein-and-Proteomics
151. SDS and urea lysis, protein degradation and freeze-thaw cycles - (reply: 4)
152. Suggestion for anti CD44 antibody for WB and IP? - (reply: 1)
153. Thrombin inactivation - (reply: 2)
154. IP - crosslinking antibody to dynabeads vs. NHS-acitvated beads? - (reply: 2)
155. Can anyone give a protocol to screen levels of adenosine deaminase in serum samp - (reply: 2)
156. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ri - (reply: 1)
157. half life of a protein - (reply: 3)
158. Western blot using blood plasma - (reply: 5)
159. PelB Leader Sequence Cleavage - (reply: 1)
160. Probing protein - (reply: 1)
161. production of phosphorylated proteins in E.coli host system - (reply: 1)
162. What is the reason of doing proteomics work? - (reply: 2)
163. Protein quantification - (reply: 4)
164. Protein extraction for 2D electrophoresis - (reply: 2)
165. Protein extraction buffer preparation (please check for accuracy) - (reply: 6)
166. Test for protein in lysate - (reply: 4)
167. co-IP stained with coomassie blue - (reply: 2)
168. Why different elution buffers for Protein G and Protein A chromatography? - (reply: 1)
169. How many PBMCs should I seed so that I can get enough protein? - (reply: 1)
170. co-ip of endogenous and tagged proteins - (reply: 1)
171. Histone extraction from S.cerevisiae - (reply: 1)
172. coomassie blue stain - (reply: 1)
173. wise to do an enzyme kinetic assay with unpurified enzyme? - (reply: 11)
174. concentation calculations - (reply: 1)
175. Vesicular Monoamine Transporter2 (VMAT2) - (reply: 8)
176. Protein sequence conservation - (reply: 2)
177. MALDI MS resutls to identify proteins - (reply: 2)
178. Dye with thiol group - (reply: 1)
179. CoIP - (reply: 2)
180. GST pull down assay: GST binds to the prey protein. Negative control shows bindi - (reply: 4)

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