Protocol Online logo
Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. Running a western with cell media in gels? - (reply: 11)
602. Transfer buffer as running buffer? - (reply: 7)
603. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
604. high background after ECL exposition - (reply: 3)
605. Circular Dichroism and disordered proteins - (reply: 1)
606. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
607. inactive enzyme from purification - (reply: 1)
608. Please comment on this Co-ip method - (reply: 2)
609. protein phosphorylation - methods for protein phosphorylation (reply: 9)
610. Co-IP of secreted proteins - (reply: 1)
611. Are protease inhibitors overrated ? - (reply: 2)
612. gst- pulldown - (reply: 1)
613. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
614. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
615. 143 kDa protein - (reply: 3)
616. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
617. Purification od insoluble protein (TIMP3) - (reply: 2)
618. descrepancy between total cell lysate and cell fractionation - (reply: 1)
619. Immunopricipitation for lysate only? - (reply: 3)
620. eluting gst fusion proteins - (reply: 1)
621. How to pull down chromatin and identify protein associated with it? - (reply: 1)
622. expression at 30 C - (reply: 1)
623. Protein extraction from perfusion fixed brain tissue - (reply: 5)
624. GST pulldown: elude or not? - (reply: 1)
625. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
626. secreted protein quantification - (reply: 2)
627. Am. Sulp Precipitation - (reply: 2)
628. EMSA - Probe Annealing problems (reply: 1)
629. Mass spectrometry for dummies? - (reply: 3)
630. Major Protein Loss! Why? - (reply: 2)