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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. expression at 30 C - (reply: 1)
602. Protein extraction from perfusion fixed brain tissue - (reply: 5)
603. GST pulldown: elude or not? - (reply: 1)
604. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
605. secreted protein quantification - (reply: 2)
606. Am. Sulp Precipitation - (reply: 2)
607. EMSA - Probe Annealing problems (reply: 1)
608. Mass spectrometry for dummies? - (reply: 3)
609. Major Protein Loss! Why? - (reply: 2)
610. Filming membrane (Western Blot & ECL) - (reply: 1)
611. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
612. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
613. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
614. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
615. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
616. protein purification - (reply: 5)
617. western blot is not consistent with immunofluorescence - (reply: 2)
618. determination of the Stoichiometry - (reply: 1)
619. cytoplasmic and nuclear extraction for BCA - (reply: 6)
620. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
621. protein prep - about prptein preperation in diagnosis (reply: 1)
622. blocking for 48hours - (reply: 1)
623. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
624. SDS PAGE - Bubble inside the Gel (reply: 5)
625. loss of protein during concentration - (reply: 6)
626. protease inhibitors and bradford - (reply: 2)
627. Cell lysates - Protein precipitation? - (reply: 3)
628. Which lysis buffer should I use? - (reply: 5)
629. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
630. cell lysate for pulldown - (reply: 3)

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