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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
602. secreted protein quantification - (reply: 2)
603. Am. Sulp Precipitation - (reply: 2)
604. EMSA - Probe Annealing problems (reply: 1)
605. Mass spectrometry for dummies? - (reply: 3)
606. Major Protein Loss! Why? - (reply: 2)
607. Filming membrane (Western Blot & ECL) - (reply: 1)
608. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
609. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
610. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
611. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
612. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
613. protein purification - (reply: 5)
614. western blot is not consistent with immunofluorescence - (reply: 2)
615. determination of the Stoichiometry - (reply: 1)
616. cytoplasmic and nuclear extraction for BCA - (reply: 6)
617. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
618. protein prep - about prptein preperation in diagnosis (reply: 1)
619. blocking for 48hours - (reply: 1)
620. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
621. SDS PAGE - Bubble inside the Gel (reply: 5)
622. loss of protein during concentration - (reply: 6)
623. protease inhibitors and bradford - (reply: 2)
624. Cell lysates - Protein precipitation? - (reply: 3)
625. Which lysis buffer should I use? - (reply: 5)
626. Samples for Western to detect phosphorylation of EGFR - (reply: 2)
627. cell lysate for pulldown - (reply: 3)
628. two hybrid system - opinion (reply: 1)
629. How many kDa add a post-translation modification? - (reply: 2)
630. making gel in advance - to save time (reply: 7)

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