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601. Problem: protein precipitates from cell lysate after defrosting - (reply: 4)
602. EMSA probes - method to make longer probes (reply: 3)
603. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
604. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
605. GST pull down - (reply: 1)
606. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
607. Determining how much enzyme to use based on activity units - (reply: 3)
608. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
609. yield vs concentration of protein - (reply: 3)
610. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
611. Sonication mysteries - (reply: 4)
612. cys rich protein expression - (reply: 1)
613. immunoprecipitation troubleshooting - (reply: 1)
614. Immunoprecipitation with two antibodies - (reply: 10)
615. RIPA Buffer precipitation trouble - (reply: 5)
616. develop western by ECL plus - (reply: 1)
617. Protein extraction from tissue - (reply: 1)
618. problem of membrane protein FPLC - (reply: 1)
619. spot removal from 2D gel and proteomics - help! (reply: 1)
620. Co-IP problem - (reply: 3)
621. I am confused, please help. - (reply: 1)
622. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
623. EMSA using biotin labeled probes and buffers - (reply: 5)
624. Foam in the BSA solution - (reply: 3)
625. Inclusion Body purification - (reply: 2)
626. How to design an antibody - (reply: 1)
627. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
628. Protein isolation from skin - (reply: 2)
629. How to concentrate my protein? - (reply: 1)
630. Organism specific protein?? - data from maldi-ms (reply: 6)
602. EMSA probes - method to make longer probes (reply: 3)
603. Does anyone use potassium acetate to precipitate protein? - or other acetate (reply: 1)
604. Preparing SDS Sample for Soluble/Insoluble Fractions - Really confused as to what the best methods to make these fractions is (reply: 8)
605. GST pull down - (reply: 1)
606. Help me convince my boss... - Calling all western blot gurus! (reply: 3)
607. Determining how much enzyme to use based on activity units - (reply: 3)
608. save me with the western blot to detect phosphorylated Jak2 and STAT4 - (reply: 3)
609. yield vs concentration of protein - (reply: 3)
610. 2d electrophoresis - Substitution of Isoelectric focusing step in 2D-Electrophoresis ??? (reply: 4)
611. Sonication mysteries - (reply: 4)
612. cys rich protein expression - (reply: 1)
613. immunoprecipitation troubleshooting - (reply: 1)
614. Immunoprecipitation with two antibodies - (reply: 10)
615. RIPA Buffer precipitation trouble - (reply: 5)
616. develop western by ECL plus - (reply: 1)
617. Protein extraction from tissue - (reply: 1)
618. problem of membrane protein FPLC - (reply: 1)
619. spot removal from 2D gel and proteomics - help! (reply: 1)
620. Co-IP problem - (reply: 3)
621. I am confused, please help. - (reply: 1)
622. Why is there extra band in IMAC SDS-PAGE - (reply: 1)
623. EMSA using biotin labeled probes and buffers - (reply: 5)
624. Foam in the BSA solution - (reply: 3)
625. Inclusion Body purification - (reply: 2)
626. How to design an antibody - (reply: 1)
627. Protein Expression in SF9/hi5 cells - troubleshooting protein expression (reply: 1)
628. Protein isolation from skin - (reply: 2)
629. How to concentrate my protein? - (reply: 1)
630. Organism specific protein?? - data from maldi-ms (reply: 6)