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601. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
602. Fusion Protein - (reply: 3)
603. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
604. Need to find a method of detection for free Histag - (reply: 1)
605. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
606. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
607. High mRNA but no protein expression - (reply: 10)
608. Running a western with cell media in gels? - (reply: 11)
609. Transfer buffer as running buffer? - (reply: 7)
610. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
611. high background after ECL exposition - (reply: 3)
612. Circular Dichroism and disordered proteins - (reply: 1)
613. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
614. inactive enzyme from purification - (reply: 1)
615. Please comment on this Co-ip method - (reply: 2)
616. protein phosphorylation - methods for protein phosphorylation (reply: 9)
617. Co-IP of secreted proteins - (reply: 1)
618. Are protease inhibitors overrated ? - (reply: 2)
619. gst- pulldown - (reply: 1)
620. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
621. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
622. 143 kDa protein - (reply: 3)
623. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
624. Purification od insoluble protein (TIMP3) - (reply: 2)
625. descrepancy between total cell lysate and cell fractionation - (reply: 1)
626. Immunopricipitation for lysate only? - (reply: 3)
627. eluting gst fusion proteins - (reply: 1)
628. How to pull down chromatin and identify protein associated with it? - (reply: 1)
629. expression at 30 C - (reply: 1)
630. Protein extraction from perfusion fixed brain tissue - (reply: 5)
602. Fusion Protein - (reply: 3)
603. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
604. Need to find a method of detection for free Histag - (reply: 1)
605. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
606. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
607. High mRNA but no protein expression - (reply: 10)
608. Running a western with cell media in gels? - (reply: 11)
609. Transfer buffer as running buffer? - (reply: 7)
610. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
611. high background after ECL exposition - (reply: 3)
612. Circular Dichroism and disordered proteins - (reply: 1)
613. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
614. inactive enzyme from purification - (reply: 1)
615. Please comment on this Co-ip method - (reply: 2)
616. protein phosphorylation - methods for protein phosphorylation (reply: 9)
617. Co-IP of secreted proteins - (reply: 1)
618. Are protease inhibitors overrated ? - (reply: 2)
619. gst- pulldown - (reply: 1)
620. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
621. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
622. 143 kDa protein - (reply: 3)
623. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
624. Purification od insoluble protein (TIMP3) - (reply: 2)
625. descrepancy between total cell lysate and cell fractionation - (reply: 1)
626. Immunopricipitation for lysate only? - (reply: 3)
627. eluting gst fusion proteins - (reply: 1)
628. How to pull down chromatin and identify protein associated with it? - (reply: 1)
629. expression at 30 C - (reply: 1)
630. Protein extraction from perfusion fixed brain tissue - (reply: 5)