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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. descrepancy between total cell lysate and cell fractionation - (reply: 1)
602. Immunopricipitation for lysate only? - (reply: 3)
603. eluting gst fusion proteins - (reply: 1)
604. How to pull down chromatin and identify protein associated with it? - (reply: 1)
605. expression at 30 C - (reply: 1)
606. Protein extraction from perfusion fixed brain tissue - (reply: 5)
607. GST pulldown: elude or not? - (reply: 1)
608. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
609. secreted protein quantification - (reply: 2)
610. Am. Sulp Precipitation - (reply: 2)
611. EMSA - Probe Annealing problems (reply: 1)
612. Mass spectrometry for dummies? - (reply: 3)
613. Major Protein Loss! Why? - (reply: 2)
614. Filming membrane (Western Blot & ECL) - (reply: 1)
615. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
616. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
617. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
618. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)
619. Effect of DTT on the affinity of Protein A/G and immune complex - Effect of DTT on the affinity of Protein A/G and immune complex (reply: 2)
620. protein purification - (reply: 5)
621. western blot is not consistent with immunofluorescence - (reply: 2)
622. determination of the Stoichiometry - (reply: 1)
623. cytoplasmic and nuclear extraction for BCA - (reply: 6)
624. Problem in expressing AKAP scaffold proteins in mammalian cells - Expression of AKAP scaffold proteins (reply: 1)
625. protein prep - about prptein preperation in diagnosis (reply: 1)
626. blocking for 48hours - (reply: 1)
627. Protein dephosphorylation with wich Phosphatase?... and what lysis buffer? - (reply: 1)
628. SDS PAGE - Bubble inside the Gel (reply: 5)
629. loss of protein during concentration - (reply: 6)
630. protease inhibitors and bradford - (reply: 2)

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