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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
602. inactive enzyme from purification - (reply: 1)
603. Please comment on this Co-ip method - (reply: 2)
604. protein phosphorylation - methods for protein phosphorylation (reply: 9)
605. Co-IP of secreted proteins - (reply: 1)
606. Are protease inhibitors overrated ? - (reply: 2)
607. gst- pulldown - (reply: 1)
608. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
609. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
610. 143 kDa protein - (reply: 3)
611. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
612. Purification od insoluble protein (TIMP3) - (reply: 2)
613. descrepancy between total cell lysate and cell fractionation - (reply: 1)
614. Immunopricipitation for lysate only? - (reply: 3)
615. eluting gst fusion proteins - (reply: 1)
616. How to pull down chromatin and identify protein associated with it? - (reply: 1)
617. expression at 30 C - (reply: 1)
618. Protein extraction from perfusion fixed brain tissue - (reply: 5)
619. GST pulldown: elude or not? - (reply: 1)
620. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
621. secreted protein quantification - (reply: 2)
622. Am. Sulp Precipitation - (reply: 2)
623. EMSA - Probe Annealing problems (reply: 1)
624. Mass spectrometry for dummies? - (reply: 3)
625. Major Protein Loss! Why? - (reply: 2)
626. Filming membrane (Western Blot & ECL) - (reply: 1)
627. why do you get vertical streaks in One dimension SDS PAGE??? - (reply: 2)
628. Homogenization for Plasma Membrane Protein Extraction - (reply: 1)
629. Questions about Blotting of Low Molecular Weight Peptide - (reply: 1)
630. Protein Gel Resolution - Unable to see proteins with a molecular weight less then 14 kDa (reply: 4)