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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. Protin helicase (urgent) - helicase activity after long term storage (reply: 3)
602. BN-PAGE - (reply: 2)
603. How can I get protein in soluble fration - (reply: 2)
604. How long does it takes to make a crystallography of a protein? - (reply: 1)
605. how to detect a knowen protein's receptor? - (reply: 1)
606. protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
607. western blot to a metal mesh - (reply: 3)
608. Protein quantification - (reply: 2)
609. Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
610. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
611. Custom Primary Antibody - (reply: 3)
612. substrate for kinase - finding the predicted substrate for a kinase (reply: 6)
613. when should I add IPTG? - (reply: 2)
614. No protein spots in 2D gel - Silver staining! - (reply: 17)
615. appropriate time for induction (adding IPTG) - (reply: 1)
616. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
617. protein crystallography services question - (reply: 1)
618. Probelm with gel filtration - (reply: 4)
619. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
620. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
621. best and simple method to purify protein - (reply: 38)
622. Pipet trypsin tips - (reply: 3)
623. Are proteases a problem in protein quantification assays? - (reply: 3)
624. serum housekeeping proteins for total protein normalization - (reply: 1)
625. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
626. Co-IP on nuclear fraction - (reply: 2)
627. Problem with dot blot - Denatured sample??? (reply: 1)
628. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
629. Odyssey Imaging and EMSA - (reply: 2)
630. 2d page - no band (reply: 2)

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