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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. best and simple method to purify protein - (reply: 38)
602. Pipet trypsin tips - (reply: 3)
603. Are proteases a problem in protein quantification assays? - (reply: 3)
604. serum housekeeping proteins for total protein normalization - (reply: 1)
605. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
606. Co-IP on nuclear fraction - (reply: 2)
607. Problem with dot blot - Denatured sample??? (reply: 1)
608. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
609. Odyssey Imaging and EMSA - (reply: 2)
610. 2d page - no band (reply: 2)
611. preparing immunoprecipitated sample for mass spec - (reply: 2)
612. Does Change pH could affect protein interaction - GST pul down (reply: 2)
613. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
614. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 7)
615. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
616. Proteasome assay - (reply: 6)
617. protein extraction - ripa buffer vs urea buffer (reply: 1)
618. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
619. EMSA - probe design and competition - (reply: 6)
620. Internal control - (reply: 1)
621. IP my protein from culture supernatant - (reply: 2)
622. High pI proteins - High pI proteins (reply: 4)
623. antibody purification - ammonium sulfate precipitation - (reply: 7)
624. wrongly used HRP-conjugated antibody in IP - what kind of result can I get (reply: 1)
625. Protein-Lipid Overlay Assay - Troubleshooting (reply: 1)
626. Protein Degradation after overexpression - (reply: 1)
627. Southern blot troubleshoot - (reply: 8)
628. IP from nuclear extract - (reply: 3)
629. Circular Dichroism - Can I use NaCl? (reply: 2)
630. SDS-PAGE: Protein bands broaden as it migrates down the gel like a pyramid! - (reply: 2)

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