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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
602. High mRNA but no protein expression - (reply: 10)
603. Running a western with cell media in gels? - (reply: 11)
604. Transfer buffer as running buffer? - (reply: 7)
605. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
606. high background after ECL exposition - (reply: 3)
607. Circular Dichroism and disordered proteins - (reply: 1)
608. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
609. inactive enzyme from purification - (reply: 1)
610. Please comment on this Co-ip method - (reply: 2)
611. protein phosphorylation - methods for protein phosphorylation (reply: 9)
612. Co-IP of secreted proteins - (reply: 1)
613. Are protease inhibitors overrated ? - (reply: 2)
614. gst- pulldown - (reply: 1)
615. what is the best way to detect protein expression levels in plants - Also which company provides the best services on antibody production? (reply: 1)
616. Refrigerator left open.....is everything ruined?? :( - (reply: 5)
617. 143 kDa protein - (reply: 3)
618. Do you get insoluble proteins also in whole cell lysate? - (reply: 3)
619. Purification od insoluble protein (TIMP3) - (reply: 2)
620. descrepancy between total cell lysate and cell fractionation - (reply: 1)
621. Immunopricipitation for lysate only? - (reply: 3)
622. eluting gst fusion proteins - (reply: 1)
623. How to pull down chromatin and identify protein associated with it? - (reply: 1)
624. expression at 30 C - (reply: 1)
625. Protein extraction from perfusion fixed brain tissue - (reply: 5)
626. GST pulldown: elude or not? - (reply: 1)
627. Nuclear extraction on non-primary culture cells - Western blot (reply: 2)
628. secreted protein quantification - (reply: 2)
629. Am. Sulp Precipitation - (reply: 2)
630. EMSA - Probe Annealing problems (reply: 1)