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Top : New Forum Archives (2009-): : Protein-and-Proteomics
601. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
602. Custom Primary Antibody - (reply: 3)
603. substrate for kinase - finding the predicted substrate for a kinase (reply: 6)
604. when should I add IPTG? - (reply: 2)
605. No protein spots in 2D gel - Silver staining! - (reply: 17)
606. appropriate time for induction (adding IPTG) - (reply: 1)
607. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
608. protein crystallography services question - (reply: 1)
609. Probelm with gel filtration - (reply: 4)
610. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
611. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
612. best and simple method to purify protein - (reply: 38)
613. Pipet trypsin tips - (reply: 3)
614. Are proteases a problem in protein quantification assays? - (reply: 3)
615. serum housekeeping proteins for total protein normalization - (reply: 1)
616. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
617. Co-IP on nuclear fraction - (reply: 2)
618. Problem with dot blot - Denatured sample??? (reply: 1)
619. Is the bubble of SDS a problem in SDS-PAGE running buffer? - (reply: 3)
620. Odyssey Imaging and EMSA - (reply: 2)
621. 2d page - no band (reply: 2)
622. preparing immunoprecipitated sample for mass spec - (reply: 2)
623. Does Change pH could affect protein interaction - GST pul down (reply: 2)
624. Incorporation of stable isotope aminoacid in protein synthesis in vitro assay - (reply: 1)
625. ELISA peptide coating problem =( - I donít know what to do to bound the peptide to the plates. (reply: 7)
626. Western Blotting of Nuclear Extracts - Does high salt inhibit western blotting? (reply: 3)
627. Proteasome assay - (reply: 6)
628. protein extraction - ripa buffer vs urea buffer (reply: 1)
629. sucrose gradient for protein fractionation? - sucrose gradient (reply: 1)
630. EMSA - probe design and competition - (reply: 6)

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