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Top : New Forum Archives (2009-): : Protein-and-Proteomics
481. Protein expression declines after overnight incubation - (reply: 4)
482. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
483. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
484. Fusion protein linker - (reply: 2)
485. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
486. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
487. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
488. Protein streaking on Blue Native-PAGE - (reply: 9)
489. basic questions about protein assays and cuvettes - (reply: 4)
490. How to attain pure chains of one protein complex - (reply: 1)
491. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
492. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
493. Fusion Protein - (reply: 3)
494. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
495. Need to find a method of detection for free Histag - (reply: 1)
496. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
497. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
498. High mRNA but no protein expression - (reply: 10)
499. Running a western with cell media in gels? - (reply: 11)
500. Transfer buffer as running buffer? - (reply: 7)
501. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
502. high background after ECL exposition - (reply: 3)
503. Circular Dichroism and disordered proteins - (reply: 1)
504. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
505. inactive enzyme from purification - (reply: 1)
506. Please comment on this Co-ip method - (reply: 2)
507. protein phosphorylation - methods for protein phosphorylation (reply: 9)
508. Co-IP of secreted proteins - (reply: 1)
509. Are protease inhibitors overrated ? - (reply: 2)
510. gst- pulldown - (reply: 1)

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