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Top : New Forum Archives (2009-): : Protein-and-Proteomics
481. BCA diluent and standard curve - (reply: 1)
482. protein induction problem - (reply: 4)
483. Growing insect cells with other cell lines - (reply: 2)
484. Primary Mouse antibody on mouse tissue - How to use mouse antibody to do western on mouse protein (reply: 2)
485. Protein expression declines after overnight incubation - (reply: 4)
486. StrepTag Missing Amino Acid - Protein Expression Tag (reply: 1)
487. Help! Cell homogenization and protein extraction for Western Blotting - (reply: 2)
488. Fusion protein linker - (reply: 2)
489. Nuclear fraction versus cytoplasmic fraction - (reply: 6)
490. common reasons proteins show up at different sizes on blot - what are they? (reply: 1)
491. Homogenization of articular cartilage prior to protein extraction - (reply: 1)
492. Protein streaking on Blue Native-PAGE - (reply: 9)
493. basic questions about protein assays and cuvettes - (reply: 4)
494. How to attain pure chains of one protein complex - (reply: 1)
495. Biotin-strptavidin pulldown experiments - :(:(:( (reply: 1)
496. Problems with precipitation of proteins using TCA/aceton - (reply: 3)
497. Fusion Protein - (reply: 3)
498. Problem with SDS-PAGE, frowny faces everywhere - (reply: 7)
499. Need to find a method of detection for free Histag - (reply: 1)
500. Blue Native PAGE - Problems with westerns of Blue Native Gels (reply: 3)
501. CoIP using Ni NTA, my non-his-tagged protein bind to beads! - (reply: 1)
502. High mRNA but no protein expression - (reply: 10)
503. Running a western with cell media in gels? - (reply: 11)
504. Transfer buffer as running buffer? - (reply: 7)
505. nuclear extraction insoluble pellet problem - here it is again! (reply: 2)
506. high background after ECL exposition - (reply: 3)
507. Circular Dichroism and disordered proteins - (reply: 1)
508. 2D-PAGE of G protein coupled receptor - Need advice on sample preparation - GPCR overexpressed in HEK293 cells (reply: 1)
509. inactive enzyme from purification - (reply: 1)
510. Please comment on this Co-ip method - (reply: 2)

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