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481. Quantitative Western Imaging - (reply: 3)
482. What causes this offset band on RIPA gel? - (reply: 4)
483. What portion of protein can I get under this protocol? - (reply: 3)
484. Squished lanes in SDS Page - (reply: 4)
485. Protin helicase (urgent) - helicase activity after long term storage (reply: 3)
486. BN-PAGE - (reply: 2)
487. How can I get protein in soluble fration - (reply: 2)
488. How long does it takes to make a crystallography of a protein? - (reply: 1)
489. how to detect a knowen protein's receptor? - (reply: 1)
490. protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
491. western blot to a metal mesh - (reply: 3)
492. Protein quantification - (reply: 2)
493. Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
494. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
495. Custom Primary Antibody - (reply: 3)
496. substrate for kinase - finding the predicted substrate for a kinase (reply: 6)
497. when should I add IPTG? - (reply: 2)
498. No protein spots in 2D gel - Silver staining! - (reply: 17)
499. appropriate time for induction (adding IPTG) - (reply: 1)
500. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
501. protein crystallography services question - (reply: 1)
502. Probelm with gel filtration - (reply: 4)
503. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
504. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
505. best and simple method to purify protein - (reply: 38)
506. Pipet trypsin tips - (reply: 3)
507. Are proteases a problem in protein quantification assays? - (reply: 3)
508. serum housekeeping proteins for total protein normalization - (reply: 1)
509. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
510. Co-IP on nuclear fraction - (reply: 2)
482. What causes this offset band on RIPA gel? - (reply: 4)
483. What portion of protein can I get under this protocol? - (reply: 3)
484. Squished lanes in SDS Page - (reply: 4)
485. Protin helicase (urgent) - helicase activity after long term storage (reply: 3)
486. BN-PAGE - (reply: 2)
487. How can I get protein in soluble fration - (reply: 2)
488. How long does it takes to make a crystallography of a protein? - (reply: 1)
489. how to detect a knowen protein's receptor? - (reply: 1)
490. protein purification - removing salts, detergents, etc from a protein sample (reply: 6)
491. western blot to a metal mesh - (reply: 3)
492. Protein quantification - (reply: 2)
493. Protein samples-best way to store - Using Denator Stabilizor T1 or other instruments/methods (reply: 1)
494. Problems when harvested cells with SDS loading buffer - extremely sticky & formed clumps (reply: 4)
495. Custom Primary Antibody - (reply: 3)
496. substrate for kinase - finding the predicted substrate for a kinase (reply: 6)
497. when should I add IPTG? - (reply: 2)
498. No protein spots in 2D gel - Silver staining! - (reply: 17)
499. appropriate time for induction (adding IPTG) - (reply: 1)
500. Help!! BCA kit v.s Bio-Rad kit for protein assay - (reply: 1)
501. protein crystallography services question - (reply: 1)
502. Probelm with gel filtration - (reply: 4)
503. c1q ? Protein A? Or Protein G for immune complex detection? - immune complex detection (reply: 2)
504. What temperature should DTT be stored (4oC or -20oC)? - (reply: 4)
505. best and simple method to purify protein - (reply: 38)
506. Pipet trypsin tips - (reply: 3)
507. Are proteases a problem in protein quantification assays? - (reply: 3)
508. serum housekeeping proteins for total protein normalization - (reply: 1)
509. inclusion body purification kit - GST tagged insoluble proteins (reply: 2)
510. Co-IP on nuclear fraction - (reply: 2)