horizontal smear? - (Dec/01/2011 )

hi

what could be the reasons for horizontal smearing in SDS-PAGE??

in general, salts, lipids, detergents, etc in the sample.

a picture and more information would help to determine the specific cause.

rick112 on Thu Dec 1 19:28:14 2011 said:

Horizontal smearing would most likely be due to too much sample loaded in the wells causing side-by-side bands to merge?