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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. reason for curved bands? - (reply: 2)
2. Inconsistent stripping - (reply: 1)
3. After Co Ip can Iuse SDS PAGE - (reply: 1)
4. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
5. BCIP/NBT developed blot disappearing - (reply: 1)
6. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
7. how to choose a proper tag for western blotting - (reply: 6)
8. Problem with thawed samples - (reply: 3)
9. Problems with histones in SDS-PAGE - (reply: 1)
10. Stripping problem - (reply: 5)
11. Problems of beta-actin - (reply: 1)
12. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
13. Problems of western signal - (reply: 5)
14. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
15. 2x laemmli buffer - (reply: 4)
16. Relative humidity in the electrophoresis lab - (reply: 3)
17. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
18. how can i explain the different pattern from my labmate's one - (reply: 8)
19. Ladder and proteins do not migrate into stacking gel - (reply: 5)
20. Western Blot: Unequal Sample Migration - (reply: 4)
21. Western blot with smeared bands - (reply: 3)
22. SDS PAGE sample preparation question, and discussion? - (reply: 3)
23. too much heating LDS-sample buffer - (reply: 3)
24. problem of cell lysate - (reply: 3)
25. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
26. Blocking agent in western blotting of histone proteins - (reply: 1)
27. Best western loading control for comparing different cell lines - (reply: 2)
28. western blotting running problem? - (reply: 2)
29. Keeping apoptotic/detached cells for WB? - (reply: 3)
30. Advice with WB please - (reply: 3)

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