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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Question with the molecular weight of my protein - (reply: 1)
2. Possible Protein Aggregation Riddle - (reply: 3)
3. How to obtain a control positive for my WB - (reply: 3)
4. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
5. Antibody not binding to low MW targets - (reply: 4)
6. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
7. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
8. Question on loss of signal between samples in western blot - (reply: 4)
9. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
10. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
11. Antibody Issues - (reply: 6)
12. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
13. Antibody-Biotinylation, detection via SDS-Gel - (reply: 11)
14. Is it possible to "over-block" a membrane? - (reply: 5)
15. Troubleshooting for BN PAGE - (reply: 6)
16. SDS PAGE Destaining - (reply: 7)
17. Layer of water above separating gel mixes into gel - (reply: 2)
18. PCR & Western Blot sample preperation - (reply: 2)
19. SDS page - (reply: 8)
20. Two-night primary antibody incubation? - (reply: 2)
21. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
22. A question about Gelanalyzer - (reply: 8)
23. storing membrane - (reply: 2)
24. band obove the ladder - (reply: 3)
25. DNA SDS-PAGE electrophoresis - (reply: 6)
26. Axin1 Co-IP - (reply: 2)
27. Glycine vs. tricine - (reply: 1)
28. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
29. SDS-PAGE power supply issues - (reply: 3)
30. Result in the lab is not the same as published - (reply: 3)

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