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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
2. BCIP/NBT developed blot disappearing - (reply: 1)
3. Western blotting - methods to detect two proteins of a similar size? - (reply: 2)
4. how to choose a proper tag for western blotting - (reply: 6)
5. Problem with thawed samples - (reply: 3)
6. Problems with histones in SDS-PAGE - (reply: 1)
7. Stripping problem - (reply: 5)
8. Problems of beta-actin - (reply: 1)
9. Problem with 15% SDS page gel during electrophoresis - (reply: 7)
10. Problems of western signal - (reply: 5)
11. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
12. 2x laemmli buffer - (reply: 4)
13. Relative humidity in the electrophoresis lab - (reply: 3)
14. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
15. how can i explain the different pattern from my labmate's one - (reply: 8)
16. Ladder and proteins do not migrate into stacking gel - (reply: 5)
17. Western Blot: Unequal Sample Migration - (reply: 4)
18. Western blot with smeared bands - (reply: 3)
19. SDS PAGE sample preparation question, and discussion? - (reply: 3)
20. too much heating LDS-sample buffer - (reply: 3)
21. problem of cell lysate - (reply: 3)
22. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
23. Blocking agent in western blotting of histone proteins - (reply: 1)
24. Best western loading control for comparing different cell lines - (reply: 2)
25. western blotting running problem? - (reply: 2)
26. Keeping apoptotic/detached cells for WB? - (reply: 3)
27. Advice with WB please - (reply: 3)
28. Western Blot Wet Transfer Conditions - (reply: 10)
29. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
30. Western Blot and Inmunofluorescence contradictions - (reply: 12)

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