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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Question on loss of signal between samples in western blot - (reply: 4)
2. IP heavy chains not in Ponceau, but in final blot exposure - (reply: 1)
3. Some bands of similar intensity aren't seen in short exposure but are seen i - (reply: 1)
4. Antibody Issues - (reply: 6)
5. Why Glycomacropeptide (GMP) does not bind Coomassie Blue stain? - (reply: 2)
6. Antibody-Biotinylation, detection via SDS-Gel - (reply: 11)
7. Is it possible to "over-block" a membrane? - (reply: 5)
8. Troubleshooting for BN PAGE - (reply: 4)
9. SDS PAGE Destaining - (reply: 7)
10. Layer of water above separating gel mixes into gel - (reply: 2)
11. PCR & Western Blot sample preperation - (reply: 2)
12. SDS page - (reply: 8)
13. Two-night primary antibody incubation? - (reply: 2)
14. Troubleshooting SDS-PAGE: turquoise Area after Coomassie staining - (reply: 2)
15. A question about Gelanalyzer - (reply: 8)
16. storing membrane - (reply: 2)
17. band obove the ladder - (reply: 3)
18. DNA SDS-PAGE electrophoresis - (reply: 2)
19. Axin1 Co-IP - (reply: 2)
20. Glycine vs. tricine - (reply: 1)
21. Smiling bands in Western blot - revisiting a widely asked question - (reply: 3)
22. SDS-PAGE power supply issues - (reply: 3)
23. Result in the lab is not the same as published - (reply: 3)
24. PKC theta - (reply: 3)
25. reason for curved bands? - (reply: 2)
26. Protein bands visible on unstained SDS-PAGE - (reply: 1)
27. Inconsistent stripping - (reply: 3)
28. After Co Ip can Iuse SDS PAGE - (reply: 1)
29. several bands in non-reducing SDS-page gel - protein without cysteines - (reply: 2)
30. BCIP/NBT developed blot disappearing - (reply: 1)

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