Protocol Online logo
Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Heat Denaturation without added denaturant (SDS or Urea) - (reply: 1)
2. Rapidly decomposing HRP substrate - (reply: 2)
3. protein size in SDS-PAGE - (reply: 4)
4. Protein not migrating through gel - (reply: 9)
5. Western Sensitivity: ECL versus DyLight 800 Detection - (reply: 1)
6. MAb detected on ELISA but no mIgG detected in WB - (reply: 1)
7. Extraction of bands from SDS-PAGE - (reply: 2)
8. Dumbbell-shaped bands on the WB - (reply: 3)
9. visualization of small peptide on tris-ricine sds page gel - (reply: 2)
10. Deformed bands in gel - (reply: 8)
11. non specific immunoblot - (reply: 2)
12. Protein stuck in the origin of resolving gel - (reply: 12)
13. detection of small peptide by the SDS-PAGE gel - (reply: 1)
14. Help in western blotting GAPDH bands are not even - (reply: 1)
15. 37kb and 7kb protein in western blot - (reply: 24)
16. Dot blot/Western background signal problem - (reply: 1)
17. Blocking and Washing membrane - (reply: 3)
18. detection of a protein band of protein complex - (reply: 3)
19. Cell lysis - (reply: 1)
20. SDS-PAGE protein analysis with antibodies alone (not WB) - (reply: 1)
21. Western blot detects exogenous protein but not endogenous - (reply: 2)
22. White bands in western blotting - (reply: 4)
23. Question with the molecular weight of my protein - (reply: 1)
24. Possible Protein Aggregation Riddle - (reply: 3)
25. How to obtain a control positive for my WB - (reply: 3)
26. Mystery of SDS gel, working with the protein GB1 (T2Q mutation) - (reply: 3)
27. Antibody not binding to low MW targets - (reply: 4)
28. Western blot problem....laddering pattern of white bands on Nitrocellulose membr - (reply: 5)
29. Problem with Western Blotting - BCA assay shows protein but no protein bands whe - (reply: 3)
30. Question on loss of signal between samples in western blot - (reply: 4)

Pages: 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35