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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. How many nitrocellulose blots can be safely stripped and incubated in primary/se - (reply: 1)
2. 2x laemmli buffer - (reply: 4)
3. Relative humidity in the electrophoresis lab - (reply: 3)
4. Almost (?) accidental use of 10X transfer buffer for 350-kDa protein - (reply: 4)
5. how can i explain the different pattern from my labmate's one - (reply: 8)
6. Ladder and proteins do not migrate into stacking gel - (reply: 5)
7. Western Blot: Unequal Sample Migration - (reply: 4)
8. Western blot with smeared bands - (reply: 3)
9. SDS PAGE sample preparation question, and discussion? - (reply: 3)
10. too much heating LDS-sample buffer - (reply: 3)
11. problem of cell lysate - (reply: 3)
12. Viral Proteins Are Not Migrating Down the Gel - (reply: 10)
13. Blocking agent in western blotting of histone proteins - (reply: 1)
14. Best western loading control for comparing different cell lines - (reply: 2)
15. western blotting running problem? - (reply: 2)
16. Keeping apoptotic/detached cells for WB? - (reply: 3)
17. Advice with WB please - (reply: 3)
18. Western Blot Wet Transfer Conditions - (reply: 10)
19. I can detect my Flag-HA tagged protein with anti-HA but not anti-Flag - (reply: 4)
20. Western Blot and Inmunofluorescence contradictions - (reply: 12)
21. Nanog double band in WB - (reply: 1)
22. My beta actin bands are changing - (reply: 6)
23. ECL Substrate Choices - (reply: 2)
24. Why does my gel have streaks instead of bands? - (reply: 2)
25. Left ECL kit at room temp. Is it ruined? - (reply: 2)
26. what do you think is the problem with this polyacrylamide gel? - (reply: 2)
27. Native PAGE problem - not entering gel enough - (reply: 8)
28. film saturation in immunoblots - (reply: 1)
29. Problems with wells on SDS PAGE gels - (reply: 3)
30. Protein is present, but not detected by antibody - (reply: 4)

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