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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Sample buffer - (reply: 1)
2. How to prep mouse urine samples for SDS-PAGE and Coomassie staining? - (reply: 2)
3. WB imaging on Vilber-Lourmat Fusion - (reply: 1)
4. Forgot to block after stripping - please send help - (reply: 1)
5. Triton-X-100 - (reply: 1)
6. How to add prepare protein sample for loading into the gel? - (reply: 3)
7. Need help with my SDS-PAGE gels - (reply: 9)
8. Transfer failure - (reply: 1)
9. Low weight protein degradation during western blot - (reply: 1)
10. white light conversion screen alternative - (reply: 1)
11. Biorad Miniprotean review - (reply: 1)
12. SDS PAGE gel length effect on resolution - (reply: 1)
13. 2D Blots problems - (reply: 6)
14. phospho-creb western blotting - (reply: 3)
15. Unspecific binding of multiple antibodies - (reply: 3)
16. gelatin zymogram - (reply: 1)
17. No or Super Strong Signal - (reply: 4)
18. Blotchy Blots - (reply: 2)
19. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
20. Dirty blots/high background - (reply: 5)
21. Heating to denature and reduce protein: same temperature? - (reply: 1)
22. Neurotoxicity of acrylamide - (reply: 2)
23. 2 bands very close in the expected place - (reply: 2)
24. Pressing Too Hard on Transfer Sandwich - (reply: 3)
25. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
26. SDS-PAGE High MW bands dissapear! - (reply: 7)
27. Non-specific binding of protein in western blot - (reply: 3)
28. SDS-PAGE vertical lane streaking - (reply: 1)
29. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
30. Storing membrane after antibody incubation - (reply: 2)

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