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Top : New Forum Archives (2009-): : SDS-PAGE-and-Western-Blotting
1. Low weight protein degradation during western blot - (reply: 1)
2. white light conversion screen alternative - (reply: 1)
3. Biorad Miniprotean review - (reply: 1)
4. SDS PAGE gel length effect on resolution - (reply: 1)
5. 2D Blots problems - (reply: 6)
6. phospho-creb western blotting - (reply: 3)
7. Unspecific binding of multiple antibodies - (reply: 3)
8. gelatin zymogram - (reply: 1)
9. No or Super Strong Signal - (reply: 4)
10. Blotchy Blots - (reply: 2)
11. Storing temperature for protein after SDS sample buffer and boiling? - (reply: 2)
12. Dirty blots/high background - (reply: 5)
13. Heating to denature and reduce protein: same temperature? - (reply: 1)
14. Neurotoxicity of acrylamide - (reply: 2)
15. 2 bands very close in the expected place - (reply: 2)
16. Pressing Too Hard on Transfer Sandwich - (reply: 3)
17. bacterial culture volume for recombinant protein to be detected in Western Blot - (reply: 3)
18. SDS-PAGE High MW bands dissapear! - (reply: 7)
19. Non-specific binding of protein in western blot - (reply: 3)
20. SDS-PAGE vertical lane streaking - (reply: 1)
21. More than 10 min exposure time in chemiluminescence detection - (reply: 2)
22. Storing membrane after antibody incubation - (reply: 2)
23. layer in between resolving and stacking gel - (reply: 1)
24. Lane spillover (?) on Western Blot - (reply: 3)
25. mRNA vs protein correlation issues, breaks central dogma - (reply: 1)
26. ECM1 on a western blot, why is one band much lower than the rest? - (reply: 1)
27. Insanely difficult time trying to cast large FACE gel for PROTEAN XI system - (reply: 1)
28. black blot images on AI600 imager, .tif shows faint bands - (reply: 1)
29. No band detected in WB - (reply: 4)
30. Concentration of Sample Buffer to boil protein samples - (reply: 2)

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