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1. Problem with GFP detection in western - (reply: 5)
2. Sudden Trouble with PVDF Transfer - (reply: 6)
3. Equal Loading Confusion - (reply: 5)
4. Wrong protein size on western blot - (reply: 10)
5. How to know if the signal of western blot is saturated ? - (reply: 4)
6. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
7. 5x SDS lysis buffer - (reply: 5)
8. Protein measurement of Laemmli samples - (reply: 2)
9. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
10. Uneven banding - Western blot troubleshooting - (reply: 4)
11. Western Blot with fixed cells - (reply: 5)
12. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
13. Primary antibody - (reply: 1)
14. Native-PAGE, help! - (reply: 3)
15. Trouble with purified recombinant protein bands - (reply: 3)
16. Stain in electrophoresis is going from + to - - (reply: 3)
17. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
18. Extraction of Transmembrane Proteins - (reply: 1)
19. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
20. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
21. Protein amount to load for publication figure? - (reply: 4)
22. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
23. What is different between Native protein and Denatured protein samples. - (reply: 4)
24. 3 extra bands show up on my western after an IP - (reply: 2)
25. IgG heavy chain from IP - (reply: 1)
26. Western Blotting loading control for large proteins - (reply: 2)
27. Uneven beta actin - (reply: 2)
28. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
29. NCOR1 western - (reply: 8)
30. Discrepancy between WB and Flow cytometry result - (reply: 5)
2. Sudden Trouble with PVDF Transfer - (reply: 6)
3. Equal Loading Confusion - (reply: 5)
4. Wrong protein size on western blot - (reply: 10)
5. How to know if the signal of western blot is saturated ? - (reply: 4)
6. Scotch-Brite versus other foam pad for wet transfer? - (reply: 3)
7. 5x SDS lysis buffer - (reply: 5)
8. Protein measurement of Laemmli samples - (reply: 2)
9. SDS Page Problem: Proteins stuck at stacking line - (reply: 3)
10. Uneven banding - Western blot troubleshooting - (reply: 4)
11. Western Blot with fixed cells - (reply: 5)
12. Mix of Milk, BSA, Casein for membrane blocking - (reply: 3)
13. Primary antibody - (reply: 1)
14. Native-PAGE, help! - (reply: 3)
15. Trouble with purified recombinant protein bands - (reply: 3)
16. Stain in electrophoresis is going from + to - - (reply: 3)
17. Would IP make the protein of interest run slower on SDS-PAGE? - (reply: 2)
18. Extraction of Transmembrane Proteins - (reply: 1)
19. Western Blot - Quantifying Tumor samples from two different blots - (reply: 2)
20. Protocol for detecting soluble, secreted proteins on a Western? - (reply: 1)
21. Protein amount to load for publication figure? - (reply: 4)
22. Native gel electrophoresis: Can it tell if a protein is forming dimer? - (reply: 1)
23. What is different between Native protein and Denatured protein samples. - (reply: 4)
24. 3 extra bands show up on my western after an IP - (reply: 2)
25. IgG heavy chain from IP - (reply: 1)
26. Western Blotting loading control for large proteins - (reply: 2)
27. Uneven beta actin - (reply: 2)
28. Minimum volume antibody solution for Western blot in tray? - (reply: 4)
29. NCOR1 western - (reply: 8)
30. Discrepancy between WB and Flow cytometry result - (reply: 5)