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SDS-PAGE-and-Western-Blotting
1.
DMSO -
(reply: 1)
2.
several questions on running SDS-PAGE -
(reply: 1)
3.
Voltage issues during transfer -
(reply: 2)
4.
problems with housekeeping antibodies when re-probing membranes -
(reply: 3)
5.
What percentage gel should I make? -
(reply: 1)
6.
Western Blot problems-vertical streaking -
(reply: 3)
7.
Protein extraction from dead cells -
(reply: 1)
8.
SDS-PAGE gel and running buffer -
(reply: 1)
9.
The same strong bands with different primary antibody -
(reply: 1)
10.
Trouble Western Blotting a 360kDa Protein -
(reply: 4)
11.
pERK and pAKT in MDA-MB-231 cells by western blot! -
(reply: 1)
12.
many bands Western Blot -
(reply: 2)
13.
degradation of signal in IP samples -
(reply: 1)
14.
Secondary Antibody Problem -
(reply: 2)
15.
LC3 Autophagy Huh7 -
(reply: 1)
16.
lysing mammalian cells in sds -
(reply: 3)
17.
Western blot mystery - possibly a sample preparation problem? -
(reply: 11)
18.
How to store a membrane? -
(reply: 1)
19.
Sample does not sit in the well despite (glycerol checked) -
(reply: 4)
20.
Western blot sample loading question -
(reply: 1)
21.
help for the method of meat sample preperation for SDS PAGE 1D -
(reply: 4)
22.
stripping too much? -
(reply: 1)
23.
sample precipitation problem in western blotting -
(reply: 2)
24.
Problem: mouse antibodies with mouse tissues -
(reply: 4)
25.
which protein can I use as a loading control for nuclear protein fraction? -
(reply: 2)
26.
Can we use ELISA capturing antibody for Western Blot Primary antibody? -
(reply: 1)
27.
MMP-9 westernblot extra bands at 25kda and 60kda -
(reply: 6)
28.
Reuse of SDS-PAGE electrode buffers -
(reply: 2)
29.
Upside-down smiley occurs when running SDS PAGE -
(reply: 3)
30.
Western transfer tank heating up -
(reply: 2)
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