gH2A.X blotting problem - (Oct/27/2011 )

Dear All,

I have a problem with blotting gH2A.X from total cell lysate (RIPA) or insoluble nuclear extracts (2%SDS in Tris). The bands look funny (see image) and I know that the problem is probably the genomic DNA. But ultrasound treatment of the sample did not help.

Does somebody have a solution to my problem?

you may have too much salt in your samples.