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Restriction enzyme Related Discussions
  1. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
  2. Primer design with EcoRI ends - (reply: 2)
  3. Does collagenase digestion require quenching? - (reply: 2)
  4. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  5. Problem with BamHI Digestion - (reply: 7)
  6. Restriction double digest - (reply: 1)
  7. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  8. Digestion bands appear faint - (reply: 3)
  9. trypsin digestion of 2D-PAGE protein spot - (reply: 2)
  10. DNA disappearing after restriction - (reply: 10)
  11. Restriction digestion and CIP - (reply: 2)
  12. Digest two sites located next to each other - (reply: 5)
  13. Looking for software that permutes codons to find new restriction sites - (reply: 4)
  14. Does a restriction enzyme cut at a related site? - (reply: 5)
  15. primer design with restriction enzyme - (reply: 1)
  16. Restriction to remove background - (reply: 4)
  17. Restriction and ligation time interval - (reply: 1)
  18. ClaI restriction enzyme efficency - (reply: 1)
  19. sequantial digest - (reply: 25)
  20. DMSO in restriction digest - (reply: 1)
  21. Partial digest XhoI NotI NEB - (reply: 6)
  22. Do you recommend Fermentas restriction enzymes? - (reply: 5)
  23. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  24. restriction enzymes/sites to avoid - (reply: 4)
  25. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
  26. Digestion necessary after PCR? - (reply: 9)
  27. Can we add a restriction site to a gene? - (reply: 1)
  28. Problem in plasmid digestion after transformation - (reply: 4)
  29. Low RNA quantity after on column DNAse digestion - (reply: 1)
  30. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  31. Glycogen affect restriction enzyme digestion? - (reply: 1)
  32. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  33. Loss of DNA after restriction digestion cleanup - (reply: 2)
  34. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  35. Restriction Digest Question - (reply: 4)
  36. Plasmid DNA digestion problem - (reply: 3)
  37. Plasmid digestion and gel purification using HindIII - (reply: 3)
  38. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  39. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  40. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  41. Colony PCR positive and Digestion negative????? - (reply: 11)
  42. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  43. different restriction enzymes different companies, can it work?? - (reply: 1)
  44. Digestion using Collagenase and dispase - (reply: 1)
  45. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  46. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  47. Question about Double Digestion followed by PCR amplification - (reply: 2)
  48. Column for removing short DNA digestion products - (reply: 6)
  49. how are cyotoxic Tcells generated, with reference to concept of MHC restriction - (reply: 2)
  50. PelB Leader Sequence Cleavage - (reply: 1)
  51. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  52. Double digestion with BamHI and BglII - (reply: 4)
  53. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  54. Problems with Digestion? - (reply: 7)
  55. plasmid digestion - problem with enzymes? - (reply: 4)
  56. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  57. Sequencing problem and restricition enzyme digestion - (reply: 9)
  58. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  59. Do I need to sequence after digestion? - (reply: 9)
  60. Problem with double digestion: one enzyme is not working - (reply: 8)
  61. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  62. Deleting restriction enzyme sites - (reply: 1)
  63. Creating primers to add restriction sites to vector backbone - (reply: 7)
  64. Restriction site rearrangement - (reply: 1)
  65. Cleavage of GST-tag with Factor Xa - (reply: 1)
  66. Restriction digest band shift? - (reply: 1)
  67. problem in cloning PCR primer design with restriction site - (reply: 4)
  68. In solution digestion - (reply: 2)
  69. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
  70. PCR and restriction enzyme digestion - (reply: 3)
  71. Probelm with double digestion - (reply: 1)
  72. How to set up simultaneous digestion? - (reply: 3)
  73. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  74. restriction enzyme - (reply: 2)
  75. SalI and double digestion. - (reply: 2)
  76. Restriction Enzyme Digest not working - (reply: 2)
  77. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  78. problem with plasmid digestion - (reply: 5)
  79. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  80. can restriction enzymes be stored at -70C? - (reply: 3)
  81. Restriction Enzymes - (reply: 4)
  82. cloning: band at different site than desired after RE digestion - (reply: 11)
  83. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  84. pcr product digestion problem - (reply: 4)
  85. Digest does not confirm presence of fragment - (reply: 5)
  86. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  87. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  88. Restriction enzymes for a gene - (reply: 2)
  89. Proteinase K digestion time. - (reply: 5)
  90. Restriction problems - (reply: 4)
  91. no product on gel after restriction digestion - (reply: 3)
  92. Plasmid Purification and Digestion Problem - (reply: 2)
  93. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  94. Vector Digestion Problem - (reply: 4)
  95. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
  96. co-IP after ubiquitin cleavage - (reply: 5)
  97. Colony PCR and digestion with KpnI - (reply: 10)
  98. Subcloning - RE digest and ligation and transformation - (reply: 5)
  99. Use PCR purification kit to purify restriction digestion - (reply: 2)
  100. No bands at all after double digestion - (reply: 1)
  101. Digest for insert orientation - (reply: 4)
  102. digestion and labelling of large ammount of DNA - (reply: 2)
  103. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
  104. His Tag Cleavage (protein precipitates) - (reply: 3)
  105. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
  106. problem of restriction enzyme digestion - (reply: 3)
  107. Simoltanious Multi-restriction digestion - (reply: 3)
  108. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  109. Restriction digestion problems - (reply: 3)
  110. Problem in Vector-Insert digestion result - (reply: 1)
  111. Digest with AarI and Pml I - (reply: 1)
  112. SDS-PAGE staining for digestion assay - (reply: 1)
  113. Problems with Restriction Digest Results - (reply: 7)
  114. Digestion - (reply: 2)
  115. Digestion of Genomic DNA - (reply: 1)
  116. Need help troubleshooting digest. - (reply: 1)
  117. Help with unknown band in restriction digest - (reply: 1)
  118. Sequential Restriction Enzyme Digestion - (reply: 1)
  119. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  120. problem in cloning involving partial digestion - (reply: 1)
  121. Why perform restriction enzyme digestion again? - (reply: 1)
  122. Restriction buffer precipitates - (reply: 3)
  123. Help with restriction analysis - (reply: 1)
  124. PARP cleavage kinetics during apoptosis - (reply: 3)
  125. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
  126. Plasmid digestion after transformation - (reply: 4)
  127. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  128. Puzzle about plamid and its digest, plz help - (reply: 8)
  129. restriction digestion buffers contaminated with dnase - (reply: 3)
  130. smear restriction digest - (reply: 1)
  131. What do you expect to see in E. coli digested with EcoRI? - (reply: 8)
  132. How to Prepare Digestion Buffer - (reply: 4)
  133. troubles in DNA digestion - (reply: 7)
  134. Enzymetic digestion with ApaI and HincII - (reply: 2)
  135. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  136. double digest problem - (reply: 3)
  137. Restriction Digest Problem - (reply: 5)
  138. Double digest after cloning doesn't give gene - (reply: 6)
  139. Restriction Site Destruction - (reply: 1)
  140. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
  141. Help in RE digestion of vector - (reply: 5)
  142. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  143. Plasmid digestion create a bigger plasmid - (reply: 1)
  144. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  145. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  146. double digestion with sac1, hindIII - (reply: 3)
  147. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  148. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  149. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  150. Help designing primers with restriction sites - (reply: 3)
  151. Restriction digestion of clones give undesired product - (reply: 3)
  152. XhoI digestion - (reply: 12)
  153. Distance between RE sites for double digestion - (reply: 5)
  154. Thrombin removel His tag protein digestion - (reply: 1)
  155. Enzyme restriction - (reply: 8)
  156. Random digest of double stranded DNA - (reply: 1)
  157. real-time PCR after restriction enzyme digestion - (reply: 6)
  158. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  159. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  160. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
  161. Double digestion with NcoI and XhoI - (reply: 3)
  162. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
  163. Restriction Enzyme sites in my genes - (reply: 1)
  164. vectors please help - vectors cleavage sites (reply: 1)
  165. 126pb insert clonnig/ digestion issues - (reply: 3)
  166. problem in digestion - (reply: 1)
  167. restriction digestion against colony PCR - (reply: 1)
  168. how long for a double digestion - (reply: 2)
  169. PCR- 3.5KB region with added histag and restriction site - Help please (reply: 3)
  170. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  171. RE digestion - (reply: 4)
  172. No bands on agarose gel after restriction digestion. - (reply: 10)
  173. metylated cloning vector - restriction digest of the insert (reply: 2)
  174. Frequency of restriction enzyme sites in a genome - (reply: 5)
  175. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  176. Digestion of mini-prep by mistake - (reply: 3)
  177. restriction digestion - (reply: 1)
  178. digestion with BamHI - SacI - molecular biology (reply: 5)
  179. C-terminal Cleavage During Overexpression - (reply: 3)
  180. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  181. restriction digest simple question - (reply: 4)
  182. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  183. bizarre digestion:) - (reply: 2)
  184. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  185. Restriction Digestion!! - (reply: 5)
  186. restriction digest - smear - (reply: 1)
  187. Adding restriction enzyme sites to sequences - (reply: 7)
  188. any strategy to insert MCS into a specific region without any restriction sites - (reply: 5)
  189. Restriction Digest Assignment Difficulty - (reply: 3)
  190. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
  191. PAS negative - amylase or diastase digestion (reply: 1)
  192. Protein cleavage and neurodegeneration. - (reply: 1)
  193. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  194. Restriction mapping - (reply: 3)
  195. gDNA restriction digest - (reply: 1)
  196. Adding Restriction Site to DNA - (reply: 3)
  197. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  198. Single Restriction Site cloning - Cloning (reply: 6)
  199. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
  200. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
  201. Digestion problem XbaI, methylation sensitive site - (reply: 3)
  202. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
  203. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
  204. cleanup of digestion before ligation - (reply: 6)
  205. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  206. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  207. Restriction digestion of gDNA for phage library - (reply: 5)
  208. No band after digestion - (reply: 4)
  209. Restriction enzyme digestion problem - (reply: 2)
  210. restriction digestion - (reply: 7)
  211. Slight Smear after Digestion - (reply: 2)
  212. Can you see any digestion? - (reply: 5)
  213. problem about pIRES2-DsRed2 double digestion - (reply: 2)
  214. digestion of insert twice and ligation of 3 bits - (reply: 6)
  215. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  216. Restriction Digest Question - regarding in silico resources (reply: 2)
  217. restriction sites to be included while calculating Tm? - (reply: 4)
  218. Double digestion and ligation problem - (reply: 1)
  219. primers with restriction site - (reply: 1)
  220. Confusion in the defination of one unit of restriction endonuclease - (reply: 1)
  221. restriction digestion - (reply: 7)
  222. Double Digest - (reply: 2)
  223. Self-ligation from a Double Digest - (reply: 4)
  224. BstUI digestion - (reply: 1)
  225. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
  226. Problem with SmaI restriction digest - (reply: 6)
  227. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
  228. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
  229. Addition of Restriction sites into PCR primers - (reply: 4)
  230. Streaky DNA digest - (reply: 3)
  231. Nco1 digest problem - (reply: 4)
  232. My battle with BamHI - (reply: 34)
  233. restriction enzyme query - (reply: 3)
  234. keeping restriction digested fragments at -20? - (reply: 5)
  235. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
  236. Cell Digestion Protocol - (reply: 1)
  237. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  238. restriction - (reply: 2)
  239. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  240. How to make digestion buffer? - (reply: 4)
  241. Problem with a Double digestion - ClaI/Esp3I digestion does not want to work! (reply: 6)
  242. addition of BclI restriction site to PCR primer - (reply: 6)
  243. restriction enzyme flanking sequence preferance - (reply: 2)
  244. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  245. Double digestion and cloning - (reply: 2)
  246. Restriction digestion - (reply: 6)
  247. Time required for digestion using Fast digest enzymes - (reply: 7)
  248. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  249. restriction digestion of PCR product - (reply: 7)
  250. Colony PCR screen positive - insert digestion negative - (reply: 2)
  251. Restriction Enzymes - Enzymes from different companies work together? (reply: 2)
  252. SNP-RFLP - problem with artifical restriction site (reply: 1)
  253. restriction problem - (reply: 3)
  254. Restriction enzyme buffer running out ? - (reply: 1)
  255. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  256. Restriction buffer composition - (reply: 2)
  257. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  258. question about restriction enzyme digest - (reply: 1)
  259. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  260. smear for digestion - (reply: 11)
  261. need help in my Fse1 restriction ezyme - (reply: 4)
  262. Restriction enzymes - (reply: 3)
  263. Need help for restriction enzymes - (reply: 5)
  264. Restriction digest - (reply: 7)
  265. Help! Double restriction digestion failed.... - (reply: 6)
  266. Digestion Protocols - (reply: 1)
  267. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  268. RE digest problem - (reply: 6)
  269. ClustalX font (for submission purpose) - Submitting manuscript, font restriction. (reply: 4)
  270. Gene insertion without desired unique restriction sites - (reply: 3)
  271. detection of restriction? - (reply: 1)
  272. The protocol for double digestion with BamHI and SacI - (reply: 15)
  273. Restriction digestion troubleshooting - (reply: 10)
  274. Which digestion enzyme? - (reply: 1)
  275. protein digestion - (reply: 2)
  276. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
  277. BamHI and HindIII double digest - driving me mad... (reply: 1)
  278. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  279. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  280. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  281. Supercoiling blocking restriction site? - (reply: 6)
  282. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
  283. Hammerhead ribozyme cleavage - (reply: 2)
  284. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
  285. Factor XA secondary cleavage sites - (reply: 1)
  286. PCR product disappears after restriction digestion - (reply: 4)
  287. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  288. Chimeric antibody Pepsin digestion - (reply: 1)
  289. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
  290. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  291. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  292. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
  293. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  294. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  295. choosing restriction sites - (reply: 5)
  296. Troubleshooting restriction digest - (reply: 1)
  297. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
  298. two step digestion - (reply: 6)
  299. BamHI restriction digest - (reply: 9)
  300. restriction enzyme problem - restriction enzyme problem (reply: 4)
  301. No product after restriction digestion - (reply: 26)
  302. prteine cleavage?? - (reply: 1)
  303. Basic doubt on restriction digestion - (reply: 2)
  304. adjacent restriction sites - (reply: 6)
  305. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  306. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
  307. Restriction endonuclease - cut only double stand DNA? (reply: 4)
  308. MBO1 digestion - (reply: 4)
  309. cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
  310. strange extra bands on digest of miniprep? - (reply: 5)
  311. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  312. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  313. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
  314. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  315. random cleavage of plasmids - insertional mutagenesis (reply: 2)
  316. Smear in my restriction digest - (reply: 12)
  317. Abnormal Nanodrop Results for pTurbo EcoRI digest - (reply: 7)
  318. Double Digest using enzymes from diff companies - (reply: 4)
  319. How to clean up restriction enzyme digestion - (reply: 8)
  320. full digestion of plasmid to get only dNTPs - (reply: 3)
  321. messed up restriction sites - (reply: 3)
  322. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  323. dpn1 digest - (reply: 5)
  324. Dnase digestion and PCR - (reply: 4)
  325. Restriction site - efficiency of restriction (reply: 4)
  326. Digestion of a plasmid - (reply: 1)
  327. different digestion pattern for same clone - (reply: 4)
  328. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  329. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  330. Troubleshooting Digest, ligation, transformation - (reply: 7)
  331. Ligation after SalI & XhoI Digestion - (reply: 3)
  332. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  333. CIP required for double digestion of plasmid? - (reply: 3)
  334. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  335. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  336. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  337. Sequential restriction digestion of plasmid - (reply: 58)
  338. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  339. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  340. problem in double digestion - (reply: 2)
  341. How to detect incomplete enzyme digests - (reply: 3)
  342. double digestion not working - (reply: 4)
  343. After double digestion, one weird band on the very top! - (reply: 4)
  344. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  345. Restriction enzyme digest - (reply: 19)