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Restriction enzyme Related Discussions
  1. Problem with restriction digestion of cloned PCR product - (reply: 5)
  2. RNase-A digestion in NEB cutsmart buffer - (reply: 1)
  3. Removal of cleaved peptide after Protease cleavage - (reply: 4)
  4. Comparing DNA fragments using restriction map - (reply: 1)
  5. Doing a double digest - (reply: 3)
  6. Problem with double digest cloning - (reply: 3)
  7. Can restriction enzymes cleave RNA? - (reply: 1)
  8. Double Digest pCD Vector - (reply: 3)
  9. Double digestion problem with PUCsp vector - (reply: 1)
  10. RE digestion and ligation troubleshooting. - (reply: 1)
  11. Insert phosphorylation or digestion - which should be done first? - (reply: 4)
  12. Primer design with EcoRI ends - (reply: 2)
  13. Does collagenase digestion require quenching? - (reply: 2)
  14. Problems with SgrAI digest, ligation, killer cut and weird bands on gel - (reply: 4)
  15. Problem with BamHI Digestion - (reply: 7)
  16. Restriction double digest - (reply: 1)
  17. PCR product and Restriction products bands are not of the expected size - (reply: 4)
  18. Digestion bands appear faint - (reply: 3)
  19. trypsin digestion of 2D-PAGE protein spot - (reply: 2)
  20. DNA disappearing after restriction - (reply: 10)
  21. Restriction digestion and CIP - (reply: 2)
  22. Digest two sites located next to each other - (reply: 5)
  23. Looking for software that permutes codons to find new restriction sites - (reply: 4)
  24. Does a restriction enzyme cut at a related site? - (reply: 5)
  25. primer design with restriction enzyme - (reply: 1)
  26. Restriction to remove background - (reply: 4)
  27. Restriction and ligation time interval - (reply: 1)
  28. ClaI restriction enzyme efficency - (reply: 1)
  29. sequantial digest - (reply: 25)
  30. DMSO in restriction digest - (reply: 1)
  31. Partial digest XhoI NotI NEB - (reply: 6)
  32. Do you recommend Fermentas restriction enzymes? - (reply: 5)
  33. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  34. restriction enzymes/sites to avoid - (reply: 4)
  35. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
  36. Digestion necessary after PCR? - (reply: 9)
  37. Can we add a restriction site to a gene? - (reply: 1)
  38. Problem in plasmid digestion after transformation - (reply: 4)
  39. Low RNA quantity after on column DNAse digestion - (reply: 1)
  40. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  41. Glycogen affect restriction enzyme digestion? - (reply: 1)
  42. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  43. Loss of DNA after restriction digestion cleanup - (reply: 2)
  44. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  45. Restriction Digest Question - (reply: 4)
  46. Plasmid DNA digestion problem - (reply: 3)
  47. Plasmid digestion and gel purification using HindIII - (reply: 3)
  48. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  49. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  50. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  51. Colony PCR positive and Digestion negative????? - (reply: 11)
  52. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  53. different restriction enzymes different companies, can it work?? - (reply: 1)
  54. Digestion using Collagenase and dispase - (reply: 1)
  55. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  56. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  57. Question about Double Digestion followed by PCR amplification - (reply: 2)
  58. Column for removing short DNA digestion products - (reply: 6)
  59. how are cyotoxic Tcells generated, with reference to concept of MHC restriction - (reply: 2)
  60. PelB Leader Sequence Cleavage - (reply: 1)
  61. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  62. Double digestion with BamHI and BglII - (reply: 4)
  63. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  64. Problems with Digestion? - (reply: 7)
  65. plasmid digestion - problem with enzymes? - (reply: 4)
  66. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  67. Sequencing problem and restricition enzyme digestion - (reply: 9)
  68. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  69. Do I need to sequence after digestion? - (reply: 9)
  70. Problem with double digestion: one enzyme is not working - (reply: 8)
  71. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  72. Deleting restriction enzyme sites - (reply: 1)
  73. Creating primers to add restriction sites to vector backbone - (reply: 7)
  74. Restriction site rearrangement - (reply: 1)
  75. Cleavage of GST-tag with Factor Xa - (reply: 1)
  76. Restriction digest band shift? - (reply: 1)
  77. problem in cloning PCR primer design with restriction site - (reply: 4)
  78. In solution digestion - (reply: 2)
  79. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
  80. PCR and restriction enzyme digestion - (reply: 3)
  81. Probelm with double digestion - (reply: 1)
  82. How to set up simultaneous digestion? - (reply: 3)
  83. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  84. restriction enzyme - (reply: 2)
  85. SalI and double digestion. - (reply: 2)
  86. Restriction Enzyme Digest not working - (reply: 2)
  87. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  88. problem with plasmid digestion - (reply: 5)
  89. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  90. can restriction enzymes be stored at -70C? - (reply: 3)
  91. Restriction Enzymes - (reply: 4)
  92. cloning: band at different site than desired after RE digestion - (reply: 11)
  93. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  94. pcr product digestion problem - (reply: 4)
  95. Digest does not confirm presence of fragment - (reply: 5)
  96. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  97. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  98. Restriction enzymes for a gene - (reply: 2)
  99. Proteinase K digestion time. - (reply: 5)
  100. Restriction problems - (reply: 4)
  101. no product on gel after restriction digestion - (reply: 3)
  102. Plasmid Purification and Digestion Problem - (reply: 2)
  103. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  104. Vector Digestion Problem - (reply: 4)
  105. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
  106. co-IP after ubiquitin cleavage - (reply: 5)
  107. Colony PCR and digestion with KpnI - (reply: 10)
  108. Subcloning - RE digest and ligation and transformation - (reply: 5)
  109. Use PCR purification kit to purify restriction digestion - (reply: 2)
  110. No bands at all after double digestion - (reply: 1)
  111. Digest for insert orientation - (reply: 4)
  112. digestion and labelling of large ammount of DNA - (reply: 2)
  113. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
  114. His Tag Cleavage (protein precipitates) - (reply: 3)
  115. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
  116. problem of restriction enzyme digestion - (reply: 3)
  117. Simoltanious Multi-restriction digestion - (reply: 3)
  118. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  119. Restriction digestion problems - (reply: 3)
  120. Problem in Vector-Insert digestion result - (reply: 1)
  121. Digest with AarI and Pml I - (reply: 1)
  122. SDS-PAGE staining for digestion assay - (reply: 1)
  123. Problems with Restriction Digest Results - (reply: 7)
  124. Digestion - (reply: 2)
  125. Digestion of Genomic DNA - (reply: 1)
  126. Need help troubleshooting digest. - (reply: 1)
  127. Help with unknown band in restriction digest - (reply: 1)
  128. Sequential Restriction Enzyme Digestion - (reply: 1)
  129. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  130. problem in cloning involving partial digestion - (reply: 1)
  131. Why perform restriction enzyme digestion again? - (reply: 1)
  132. Restriction buffer precipitates - (reply: 3)
  133. Help with restriction analysis - (reply: 1)
  134. PARP cleavage kinetics during apoptosis - (reply: 3)
  135. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
  136. Plasmid digestion after transformation - (reply: 4)
  137. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  138. Puzzle about plamid and its digest, plz help - (reply: 8)
  139. restriction digestion buffers contaminated with dnase - (reply: 3)
  140. smear restriction digest - (reply: 1)
  141. What do you expect to see in E. coli digested with EcoRI? - (reply: 8)
  142. How to Prepare Digestion Buffer - (reply: 4)
  143. troubles in DNA digestion - (reply: 7)
  144. Enzymetic digestion with ApaI and HincII - (reply: 2)
  145. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  146. double digest problem - (reply: 3)
  147. Restriction Digest Problem - (reply: 5)
  148. Double digest after cloning doesn't give gene - (reply: 6)
  149. Restriction Site Destruction - (reply: 1)
  150. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
  151. Help in RE digestion of vector - (reply: 5)
  152. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  153. Plasmid digestion create a bigger plasmid - (reply: 1)
  154. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  155. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  156. double digestion with sac1, hindIII - (reply: 3)
  157. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  158. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  159. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  160. Help designing primers with restriction sites - (reply: 3)
  161. Restriction digestion of clones give undesired product - (reply: 3)
  162. XhoI digestion - (reply: 12)
  163. Distance between RE sites for double digestion - (reply: 5)
  164. Thrombin removel His tag protein digestion - (reply: 1)
  165. Enzyme restriction - (reply: 8)
  166. Random digest of double stranded DNA - (reply: 1)
  167. real-time PCR after restriction enzyme digestion - (reply: 6)
  168. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  169. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  170. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
  171. Double digestion with NcoI and XhoI - (reply: 3)
  172. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
  173. Restriction Enzyme sites in my genes - (reply: 1)
  174. vectors please help - vectors cleavage sites (reply: 1)
  175. 126pb insert clonnig/ digestion issues - (reply: 3)
  176. problem in digestion - (reply: 1)
  177. restriction digestion against colony PCR - (reply: 1)
  178. how long for a double digestion - (reply: 2)
  179. PCR- 3.5KB region with added histag and restriction site - Help please (reply: 3)
  180. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  181. RE digestion - (reply: 4)
  182. No bands on agarose gel after restriction digestion. - (reply: 10)
  183. metylated cloning vector - restriction digest of the insert (reply: 2)
  184. Frequency of restriction enzyme sites in a genome - (reply: 5)
  185. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  186. Digestion of mini-prep by mistake - (reply: 3)
  187. restriction digestion - (reply: 1)
  188. digestion with BamHI - SacI - molecular biology (reply: 5)
  189. C-terminal Cleavage During Overexpression - (reply: 3)
  190. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  191. restriction digest simple question - (reply: 4)
  192. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  193. bizarre digestion:) - (reply: 2)
  194. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  195. Restriction Digestion!! - (reply: 5)
  196. restriction digest - smear - (reply: 1)
  197. Adding restriction enzyme sites to sequences - (reply: 7)
  198. any strategy to insert MCS into a specific region without any restriction sites - (reply: 5)
  199. Restriction Digest Assignment Difficulty - (reply: 3)
  200. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
  201. PAS negative - amylase or diastase digestion (reply: 1)
  202. Protein cleavage and neurodegeneration. - (reply: 1)
  203. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  204. Restriction mapping - (reply: 3)
  205. gDNA restriction digest - (reply: 1)
  206. Adding Restriction Site to DNA - (reply: 3)
  207. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  208. Single Restriction Site cloning - Cloning (reply: 6)
  209. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
  210. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
  211. Digestion problem XbaI, methylation sensitive site - (reply: 3)
  212. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
  213. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
  214. cleanup of digestion before ligation - (reply: 6)
  215. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  216. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  217. Restriction digestion of gDNA for phage library - (reply: 5)
  218. No band after digestion - (reply: 4)
  219. Restriction enzyme digestion problem - (reply: 2)
  220. restriction digestion - (reply: 7)
  221. Slight Smear after Digestion - (reply: 2)
  222. Can you see any digestion? - (reply: 5)
  223. problem about pIRES2-DsRed2 double digestion - (reply: 2)
  224. digestion of insert twice and ligation of 3 bits - (reply: 6)
  225. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  226. Restriction Digest Question - regarding in silico resources (reply: 2)
  227. restriction sites to be included while calculating Tm? - (reply: 4)
  228. Double digestion and ligation problem - (reply: 1)
  229. primers with restriction site - (reply: 1)
  230. Confusion in the defination of one unit of restriction endonuclease - (reply: 1)
  231. restriction digestion - (reply: 7)
  232. Double Digest - (reply: 2)
  233. Self-ligation from a Double Digest - (reply: 4)
  234. BstUI digestion - (reply: 1)
  235. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
  236. Problem with SmaI restriction digest - (reply: 6)
  237. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
  238. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
  239. Addition of Restriction sites into PCR primers - (reply: 4)
  240. Streaky DNA digest - (reply: 3)
  241. Nco1 digest problem - (reply: 4)
  242. My battle with BamHI - (reply: 35)
  243. restriction enzyme query - (reply: 3)
  244. keeping restriction digested fragments at -20? - (reply: 5)
  245. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
  246. Cell Digestion Protocol - (reply: 1)
  247. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  248. restriction - (reply: 2)
  249. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  250. How to make digestion buffer? - (reply: 4)
  251. Problem with a Double digestion - ClaI/Esp3I digestion does not want to work! (reply: 6)
  252. addition of BclI restriction site to PCR primer - (reply: 6)
  253. restriction enzyme flanking sequence preferance - (reply: 2)
  254. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  255. Double digestion and cloning - (reply: 2)
  256. Restriction digestion - (reply: 6)
  257. Time required for digestion using Fast digest enzymes - (reply: 7)
  258. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  259. restriction digestion of PCR product - (reply: 7)
  260. Colony PCR screen positive - insert digestion negative - (reply: 2)
  261. Restriction Enzymes - Enzymes from different companies work together? (reply: 2)
  262. SNP-RFLP - problem with artifical restriction site (reply: 1)
  263. restriction problem - (reply: 3)
  264. Restriction enzyme buffer running out ? - (reply: 1)
  265. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  266. Restriction buffer composition - (reply: 2)
  267. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  268. question about restriction enzyme digest - (reply: 1)
  269. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  270. smear for digestion - (reply: 11)
  271. need help in my Fse1 restriction ezyme - (reply: 4)
  272. Restriction enzymes - (reply: 3)
  273. Need help for restriction enzymes - (reply: 5)
  274. Restriction digest - (reply: 7)
  275. Help! Double restriction digestion failed.... - (reply: 6)
  276. Digestion Protocols - (reply: 1)
  277. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  278. RE digest problem - (reply: 6)
  279. ClustalX font (for submission purpose) - Submitting manuscript, font restriction. (reply: 4)
  280. Gene insertion without desired unique restriction sites - (reply: 3)
  281. detection of restriction? - (reply: 1)
  282. The protocol for double digestion with BamHI and SacI - (reply: 15)
  283. Restriction digestion troubleshooting - (reply: 10)
  284. Which digestion enzyme? - (reply: 1)
  285. protein digestion - (reply: 2)
  286. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
  287. BamHI and HindIII double digest - driving me mad... (reply: 1)
  288. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  289. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  290. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  291. Supercoiling blocking restriction site? - (reply: 6)
  292. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
  293. Hammerhead ribozyme cleavage - (reply: 2)
  294. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
  295. Factor XA secondary cleavage sites - (reply: 1)
  296. PCR product disappears after restriction digestion - (reply: 4)
  297. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  298. Chimeric antibody Pepsin digestion - (reply: 1)
  299. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
  300. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  301. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  302. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
  303. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  304. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  305. choosing restriction sites - (reply: 5)
  306. Troubleshooting restriction digest - (reply: 1)
  307. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
  308. two step digestion - (reply: 6)
  309. BamHI restriction digest - (reply: 9)
  310. restriction enzyme problem - restriction enzyme problem (reply: 4)
  311. No product after restriction digestion - (reply: 26)
  312. prteine cleavage?? - (reply: 1)
  313. Basic doubt on restriction digestion - (reply: 2)
  314. adjacent restriction sites - (reply: 6)
  315. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  316. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
  317. Restriction endonuclease - cut only double stand DNA? (reply: 4)
  318. MBO1 digestion - (reply: 4)
  319. cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
  320. strange extra bands on digest of miniprep? - (reply: 5)
  321. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  322. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  323. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
  324. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  325. random cleavage of plasmids - insertional mutagenesis (reply: 2)
  326. Smear in my restriction digest - (reply: 12)
  327. Abnormal Nanodrop Results for pTurbo EcoRI digest - (reply: 7)
  328. Double Digest using enzymes from diff companies - (reply: 4)
  329. How to clean up restriction enzyme digestion - (reply: 8)
  330. full digestion of plasmid to get only dNTPs - (reply: 3)
  331. messed up restriction sites - (reply: 3)
  332. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  333. dpn1 digest - (reply: 5)
  334. Dnase digestion and PCR - (reply: 4)
  335. Restriction site - efficiency of restriction (reply: 4)
  336. Digestion of a plasmid - (reply: 1)
  337. different digestion pattern for same clone - (reply: 4)
  338. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  339. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  340. Troubleshooting Digest, ligation, transformation - (reply: 7)
  341. Ligation after SalI & XhoI Digestion - (reply: 3)
  342. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  343. CIP required for double digestion of plasmid? - (reply: 3)
  344. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  345. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  346. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  347. Sequential restriction digestion of plasmid - (reply: 58)
  348. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  349. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  350. problem in double digestion - (reply: 2)
  351. How to detect incomplete enzyme digests - (reply: 3)
  352. double digestion not working - (reply: 4)
  353. After double digestion, one weird band on the very top! - (reply: 4)
  354. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  355. Restriction enzyme digest - (reply: 19)