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Restriction enzyme Related Discussions
  1. Digestion bands appear faint - (reply: 1)
  2. DNA disappearing after restriction - (reply: 10)
  3. Restriction digestion and CIP - (reply: 2)
  4. Digest two sites located next to each other - (reply: 5)
  5. Looking for software that permutes codons to find new restriction sites - (reply: 4)
  6. Does a restriction enzyme cut at a related site? - (reply: 5)
  7. primer design with restriction enzyme - (reply: 1)
  8. Restriction to remove background - (reply: 4)
  9. Restriction and ligation time interval - (reply: 1)
  10. ClaI restriction enzyme efficency - (reply: 1)
  11. sequantial digest - (reply: 25)
  12. DMSO in restriction digest - (reply: 1)
  13. Partial digest XhoI NotI NEB - (reply: 6)
  14. Do you recommend Fermentas restriction enzymes? - (reply: 4)
  15. Enzyme digestion resulted in extra bands from plasmid miniprep product - (reply: 10)
  16. restriction enzymes/sites to avoid - (reply: 4)
  17. Designing cloning primes (restriction site and kozak sequence) - (reply: 1)
  18. Digestion necessary after PCR? - (reply: 7)
  19. Can we add a restriction site to a gene? - (reply: 1)
  20. Problem in plasmid digestion after transformation - (reply: 4)
  21. Low RNA quantity after on column DNAse digestion - (reply: 1)
  22. Small yield of RNA after on column DNAs digestion , Why - (reply: 3)
  23. Glycogen affect restriction enzyme digestion? - (reply: 1)
  24. Why loading buffer can't go down after enzyme digestion? - (reply: 1)
  25. Loss of DNA after restriction digestion cleanup - (reply: 2)
  26. primer checking & restriction enzyme based methylation specific polymerase c - (reply: 8)
  27. Restriction Digest Question - (reply: 4)
  28. Plasmid DNA digestion problem - (reply: 3)
  29. Plasmid digestion and gel purification using HindIII - (reply: 3)
  30. New ordered Restriction Enzymes not working, need help! - (reply: 2)
  31. Verified Plasmids(via digestion) > Transfection and Visible GFP > GFP de - (reply: 2)
  32. RNase digestion followed by Radiolabelling iCLIP/CLIP - (reply: 1)
  33. Colony PCR positive and Digestion negative????? - (reply: 11)
  34. No insert for PCR cloning and restriction enzyme digestion - (reply: 4)
  35. different restriction enzymes different companies, can it work?? - (reply: 1)
  36. Digestion using Collagenase and dispase - (reply: 1)
  37. Help! Plasmid resistant to restriction digest after acquiring Flag tag - (reply: 3)
  38. Question about DNA purification from a restriction digestion reaction - (reply: 1)
  39. Question about Double Digestion followed by PCR amplification - (reply: 2)
  40. Column for removing short DNA digestion products - (reply: 6)
  41. how are cyotoxic Tcells generated, with reference to concept of MHC restriction - (reply: 2)
  42. PelB Leader Sequence Cleavage - (reply: 1)
  43. Use magnetic beads to purify restriction enzyme digestion product - (reply: 1)
  44. Double digestion with BamHI and BglII - (reply: 4)
  45. Digestion of pRSETA vector leads to funny migration profile - (reply: 2)
  46. Problems with Digestion? - (reply: 7)
  47. plasmid digestion - problem with enzymes? - (reply: 4)
  48. classic cloning with restriction enzymes into empty pcDNA3.1D - (reply: 1)
  49. Sequencing problem and restricition enzyme digestion - (reply: 9)
  50. Reappearing band shifts/smears after double digest and gel purification - (reply: 2)
  51. Do I need to sequence after digestion? - (reply: 9)
  52. Problem with double digestion: one enzyme is not working - (reply: 8)
  53. PCR amplification with new restriction sites troubleshooting - (reply: 2)
  54. Deleting restriction enzyme sites - (reply: 1)
  55. Creating primers to add restriction sites to vector backbone - (reply: 7)
  56. Restriction site rearrangement - (reply: 1)
  57. Cleavage of GST-tag with Factor Xa - (reply: 1)
  58. Restriction digest band shift? - (reply: 1)
  59. problem in cloning PCR primer design with restriction site - (reply: 4)
  60. In solution digestion - (reply: 2)
  61. Partial cleavage of HA (Hemagglutinin) tag? - (reply: 4)
  62. PCR and restriction enzyme digestion - (reply: 3)
  63. Probelm with double digestion - (reply: 1)
  64. How to set up simultaneous digestion? - (reply: 3)
  65. adding Nde1 and Xho 1 restriction site in PCR primer - (reply: 2)
  66. restriction enzyme - (reply: 2)
  67. SalI and double digestion. - (reply: 2)
  68. Restriction Enzyme Digest not working - (reply: 2)
  69. Incomplete digestion of plasmid with single enzyme - (reply: 1)
  70. problem with plasmid digestion - (reply: 5)
  71. Do you have to purify target vector after double digest from MCS piece - (reply: 1)
  72. can restriction enzymes be stored at -70C? - (reply: 3)
  73. Restriction Enzymes - (reply: 4)
  74. cloning: band at different site than desired after RE digestion - (reply: 11)
  75. Restriction enzyme - PCR sheep blood gDNA - (reply: 5)
  76. pcr product digestion problem - (reply: 4)
  77. Digest does not confirm presence of fragment - (reply: 5)
  78. Does Proteinase K digest RNAse in lysis buffer? - (reply: 2)
  79. Smear after digestion of a plasmid from a Dam- strain - (reply: 1)
  80. Restriction enzymes for a gene - (reply: 2)
  81. Proteinase K digestion time. - (reply: 5)
  82. Restriction problems - (reply: 4)
  83. no product on gel after restriction digestion - (reply: 3)
  84. Plasmid Purification and Digestion Problem - (reply: 2)
  85. mass spectrometry and tryptic digestion on membrane proteins - (reply: 5)
  86. Vector Digestion Problem - (reply: 4)
  87. Peptide recuperation after FactorXa cleavage using GST fusion - (reply: 1)
  88. co-IP after ubiquitin cleavage - (reply: 5)
  89. Colony PCR and digestion with KpnI - (reply: 10)
  90. Subcloning - RE digest and ligation and transformation - (reply: 5)
  91. Use PCR purification kit to purify restriction digestion - (reply: 2)
  92. No bands at all after double digestion - (reply: 1)
  93. Digest for insert orientation - (reply: 4)
  94. digestion and labelling of large ammount of DNA - (reply: 2)
  95. Digestion with EcoRI and NdeI - any tips? - (reply: 5)
  96. His Tag Cleavage (protein precipitates) - (reply: 3)
  97. Did my EcoRI restriction digestion work? picture included! - (reply: 49)
  98. problem of restriction enzyme digestion - (reply: 3)
  99. Simoltanious Multi-restriction digestion - (reply: 3)
  100. Using unopened restriction enzyme "expired" in 2010/11 - (reply: 3)
  101. Restriction digestion problems - (reply: 3)
  102. Problem in Vector-Insert digestion result - (reply: 1)
  103. Digest with AarI and Pml I - (reply: 1)
  104. SDS-PAGE staining for digestion assay - (reply: 1)
  105. Problems with Restriction Digest Results - (reply: 7)
  106. Digestion - (reply: 2)
  107. Digestion of Genomic DNA - (reply: 1)
  108. Need help troubleshooting digest. - (reply: 1)
  109. Help with unknown band in restriction digest - (reply: 1)
  110. Sequential Restriction Enzyme Digestion - (reply: 1)
  111. Primers for Introduction of new restriction sites to a vector - (reply: 3)
  112. problem in cloning involving partial digestion - (reply: 1)
  113. Why perform restriction enzyme digestion again? - (reply: 1)
  114. Restriction buffer precipitates - (reply: 3)
  115. Help with restriction analysis - (reply: 1)
  116. PARP cleavage kinetics during apoptosis - (reply: 3)
  117. Why do double digest (XhoI & SacI) of NEB use buffer 1 ? - (reply: 4)
  118. Plasmid digestion after transformation - (reply: 4)
  119. PCRed on restriction sites -- how do I purify it? - (reply: 8)
  120. Puzzle about plamid and its digest, plz help - (reply: 8)
  121. restriction digestion buffers contaminated with dnase - (reply: 3)
  122. smear restriction digest - (reply: 1)
  123. What do you expect to see in E. coli digested with EcoRI? - (reply: 8)
  124. How to Prepare Digestion Buffer - (reply: 4)
  125. troubles in DNA digestion - (reply: 7)
  126. Enzymetic digestion with ApaI and HincII - (reply: 2)
  127. I've run out of ideas.... Restriction enzyme digestion - (reply: 6)
  128. double digest problem - (reply: 3)
  129. Restriction Digest Problem - (reply: 5)
  130. Double digest after cloning doesn't give gene - (reply: 6)
  131. Restriction Site Destruction - (reply: 1)
  132. Problem with restriction digestion of agarose-embedded DNA - (reply: 4)
  133. Help in RE digestion of vector - (reply: 5)
  134. How to add restriction enzyme site into the DNA fragment - (reply: 3)
  135. Plasmid digestion create a bigger plasmid - (reply: 1)
  136. restricted DNA from the enzymes Bam H1, Hind111, Eco R1 and Sau 3A fall in? - (reply: 1)
  137. How do I insert a DNA sequence into a multiple cloning site and add restriction - (reply: 1)
  138. double digestion with sac1, hindIII - (reply: 3)
  139. Restriction Enzyme Inactivation & Pfu PCR - (reply: 2)
  140. help in digesting DNA fragment using restriction enzyme - (reply: 2)
  141. useing a uniqeu enzyme for both insert and vector digestion - (reply: 14)
  142. Help designing primers with restriction sites - (reply: 3)
  143. Restriction digestion of clones give undesired product - (reply: 3)
  144. XhoI digestion - (reply: 12)
  145. Distance between RE sites for double digestion - (reply: 5)
  146. Thrombin removel His tag protein digestion - (reply: 1)
  147. Enzyme restriction - (reply: 8)
  148. Random digest of double stranded DNA - (reply: 1)
  149. real-time PCR after restriction enzyme digestion - (reply: 6)
  150. how to purify Fc fragment - Fc fragment purification after papain digestion (reply: 8)
  151. Cloning Difficulty - Problems with digestion/ligation (reply: 3)
  152. Sequential digestion with SmaI and EcoRI...... - (reply: 5)
  153. Double digestion with NcoI and XhoI - (reply: 3)
  154. What is the expected pattern of restriction digestion of human genomic DNA? - (reply: 2)
  155. Restriction Enzyme sites in my genes - (reply: 1)
  156. vectors please help - vectors cleavage sites (reply: 1)
  157. 126pb insert clonnig/ digestion issues - (reply: 3)
  158. problem in digestion - (reply: 1)
  159. restriction digestion against colony PCR - (reply: 1)
  160. how long for a double digestion - (reply: 2)
  161. PCR- 3.5KB region with added histag and restriction site - Help please (reply: 3)
  162. DNA purification from restriction digest - It keeps on degrading :( :( :( (reply: 4)
  163. RE digestion - (reply: 4)
  164. No bands on agarose gel after restriction digestion. - (reply: 10)
  165. metylated cloning vector - restriction digest of the insert (reply: 2)
  166. Frequency of restriction enzyme sites in a genome - (reply: 5)
  167. positive colony PCR, negative restriction digest, positive PC from minipreps - Cloning nightmare (reply: 2)
  168. Digestion of mini-prep by mistake - (reply: 3)
  169. restriction digestion - (reply: 1)
  170. digestion with BamHI - SacI - molecular biology (reply: 5)
  171. C-terminal Cleavage During Overexpression - (reply: 3)
  172. Strange problem with digestion and dephosporylation of PCR product - (reply: 2)
  173. restriction digest simple question - (reply: 4)
  174. Digestion of clinical plasmids for posterior cloning - Digestion of clinical plasmids for posterior cloning (reply: 2)
  175. bizarre digestion:) - (reply: 2)
  176. Digestion and ligation problem! Need advice - How to handle this situation (reply: 1)
  177. Restriction Digestion!! - (reply: 5)
  178. restriction digest - smear - (reply: 1)
  179. Adding restriction enzyme sites to sequences - (reply: 7)
  180. any strategy to insert MCS into a specific region without any restriction sites - (reply: 5)
  181. Restriction Digest Assignment Difficulty - (reply: 3)
  182. pBR322 restriction digest - PBR322 digest with EcoRV and BstZ17I (reply: 20)
  183. PAS negative - amylase or diastase digestion (reply: 1)
  184. Protein cleavage and neurodegeneration. - (reply: 1)
  185. Dna cloning using NdeI/BamHI using pET11 vector - (reply: 4)
  186. Restriction mapping - (reply: 3)
  187. gDNA restriction digest - (reply: 1)
  188. Adding Restriction Site to DNA - (reply: 3)
  189. Dye in PCR Buffer - Inhibiting downstream techniques? - ligation/digestion/cloning (reply: 7)
  190. Single Restriction Site cloning - Cloning (reply: 6)
  191. EcoRI cloning giving only inverted inserts!!?? - (reply: 3)
  192. BamHI and SacI double digest - 100% activity in NEBuffer 4 but why not recommended? (reply: 5)
  193. Digestion problem XbaI, methylation sensitive site - (reply: 3)
  194. what is the optimal temperature for my restriction enzyme - Urgent plea (reply: 3)
  195. Identifying unknown cDNA inserts from restriction digests - (reply: 2)
  196. cleanup of digestion before ligation - (reply: 6)
  197. How to extract restriction enzyme (or restriction endonuclease) from bacteria? - (reply: 1)
  198. DNAse digestion while RNA extraction with TRIzol - anyone tried that before? (reply: 1)
  199. Restriction digestion of gDNA for phage library - (reply: 5)
  200. No band after digestion - (reply: 4)
  201. Restriction enzyme digestion problem - (reply: 2)
  202. restriction digestion - (reply: 7)
  203. Slight Smear after Digestion - (reply: 2)
  204. Can you see any digestion? - (reply: 5)
  205. problem about pIRES2-DsRed2 double digestion - (reply: 2)
  206. digestion of insert twice and ligation of 3 bits - (reply: 6)
  207. AvrII??? Friend or Foe? - molecular cloning; AvrII; digestion; resistance (reply: 1)
  208. Restriction Digest Question - regarding in silico resources (reply: 2)
  209. restriction sites to be included while calculating Tm? - (reply: 4)
  210. Double digestion and ligation problem - (reply: 1)
  211. primers with restriction site - (reply: 1)
  212. Confusion in the defination of one unit of restriction endonuclease - (reply: 1)
  213. restriction digestion - (reply: 7)
  214. Double Digest - (reply: 2)
  215. Self-ligation from a Double Digest - (reply: 4)
  216. BstUI digestion - (reply: 1)
  217. KpnI Restriction enzyme - how to detect the SNP (reply: 1)
  218. Problem with SmaI restriction digest - (reply: 6)
  219. Double digestion using EcoRI and HindIII with pHAT-CAT - (reply: 4)
  220. What is the minimum DNA concentration requried for double digestion? - (reply: 1)
  221. Addition of Restriction sites into PCR primers - (reply: 4)
  222. Streaky DNA digest - (reply: 3)
  223. Nco1 digest problem - (reply: 4)
  224. My battle with BamHI - (reply: 34)
  225. restriction enzyme query - (reply: 3)
  226. keeping restriction digested fragments at -20? - (reply: 5)
  227. partial DNA digestion formula - Linear DNA partial digestion (reply: 1)
  228. Cell Digestion Protocol - (reply: 1)
  229. Equalizing RNA samples for Reverse Transcription - Should I do this before or after DNA digestion (reply: 2)
  230. restriction - (reply: 2)
  231. To clean or not to clean.....? - PCR product clean up prior to restriction enzyme digestion (reply: 3)
  232. How to make digestion buffer? - (reply: 4)
  233. Problem with a Double digestion - ClaI/Esp3I digestion does not want to work! (reply: 6)
  234. addition of BclI restriction site to PCR primer - (reply: 6)
  235. restriction enzyme flanking sequence preferance - (reply: 2)
  236. screening of positive clones - colony PCR /Plasmid isolation and Restriction digestion (reply: 5)
  237. Double digestion and cloning - (reply: 2)
  238. Restriction digestion - (reply: 6)
  239. Time required for digestion using Fast digest enzymes - (reply: 7)
  240. Incompatible restriction sites - Will E.coli ligate for me? (reply: 2)
  241. restriction digestion of PCR product - (reply: 7)
  242. Colony PCR screen positive - insert digestion negative - (reply: 2)
  243. Restriction Enzymes - Enzymes from different companies work together? (reply: 2)
  244. SNP-RFLP - problem with artifical restriction site (reply: 1)
  245. restriction problem - (reply: 3)
  246. Restriction enzyme buffer running out ? - (reply: 1)
  247. AdEasy Vector System: Why PacI digestion? - (reply: 1)
  248. Restriction buffer composition - (reply: 2)
  249. overnight restriction digestion- good or bad? - Should we modify amount of enzyme added for overnight digests? (reply: 12)
  250. question about restriction enzyme digest - (reply: 1)
  251. how to get sharp band for restriction enzyme - how to get sharp band for restriction enzyme (reply: 2)
  252. smear for digestion - (reply: 11)
  253. need help in my Fse1 restriction ezyme - (reply: 4)
  254. Restriction enzymes - (reply: 3)
  255. Need help for restriction enzymes - (reply: 5)
  256. Restriction digest - (reply: 7)
  257. Help! Double restriction digestion failed.... - (reply: 6)
  258. Digestion Protocols - (reply: 1)
  259. HELP! Can't double digest pcDNA3.1 vector - (reply: 6)
  260. RE digest problem - (reply: 6)
  261. ClustalX font (for submission purpose) - Submitting manuscript, font restriction. (reply: 4)
  262. Gene insertion without desired unique restriction sites - (reply: 3)
  263. detection of restriction? - (reply: 1)
  264. The protocol for double digestion with BamHI and SacI - (reply: 15)
  265. Restriction digestion troubleshooting - (reply: 10)
  266. Which digestion enzyme? - (reply: 1)
  267. protein digestion - (reply: 2)
  268. How To Fix NaOH/Tris DNA Digest Error? - (reply: 1)
  269. BamHI and HindIII double digest - driving me mad... (reply: 1)
  270. Brain tumour tissue enzymatic digestion for culturing cells - (reply: 2)
  271. NEB Restriction Enzymes and Buffer - Restriction (reply: 13)
  272. Inversed PCR issues - DNA isolation, restriction, ligation and PCR with no success (reply: 1)
  273. Supercoiling blocking restriction site? - (reply: 6)
  274. Low Thrombin Cleavage Efficiency Problem - I am trying to cleave a GST tagged protein(about 35 kDa + 26 kDa GST) (reply: 1)
  275. Hammerhead ribozyme cleavage - (reply: 2)
  276. Throbin cleavage declines with older protein? - It used to work, now doesn't! (reply: 1)
  277. Factor XA secondary cleavage sites - (reply: 1)
  278. PCR product disappears after restriction digestion - (reply: 4)
  279. Which are the cheap restriction enzymes? - Does anyone have a list? (reply: 5)
  280. Chimeric antibody Pepsin digestion - (reply: 1)
  281. restriction digestion - how long are restriction overhangs intact?? (reply: 3)
  282. Control Restriction Digestion - got one additional band - what does this mean? (reply: 5)
  283. Restriction Enzyme Digest of Genomic DNA - How to get complete digestion? (reply: 9)
  284. 5-aza-2'-deoxycytidine inhibits digest? - Can this base analog inhibit RE-digest? (reply: 1)
  285. Restriction Enzyme Digest of Genomic DNA - Problem with RE-digest + PCR in CpG island assay (reply: 1)
  286. Is gDNA digestion before bisulfite conversion important? - (reply: 5)
  287. choosing restriction sites - (reply: 5)
  288. Troubleshooting restriction digest - (reply: 1)
  289. Xho1 doesn't digest pEGFP.N2 completely, why? - (reply: 6)
  290. two step digestion - (reply: 6)
  291. BamHI restriction digest - (reply: 9)
  292. restriction enzyme problem - restriction enzyme problem (reply: 4)
  293. No product after restriction digestion - (reply: 26)
  294. prteine cleavage?? - (reply: 1)
  295. Basic doubt on restriction digestion - (reply: 2)
  296. adjacent restriction sites - (reply: 6)
  297. restriction digestion of PURIFIED PRODUCT - (reply: 5)
  298. comparison of bisulfite sequencing and base specific cleavage coupled with Mass - (reply: 5)
  299. Restriction endonuclease - cut only double stand DNA? (reply: 4)
  300. MBO1 digestion - (reply: 4)
  301. cDNA synthesis - need amplified cDNA to do RE digestion (reply: 6)
  302. strange extra bands on digest of miniprep? - (reply: 5)
  303. need help with agarose gel electrophoresis and restriction enzyme - (reply: 7)
  304. looking for a vector with a Neo cassette, HindIII and BamHI restriction sites (i - (reply: 7)
  305. sequential digest trouble with pUC 19 and XbaI - (reply: 1)
  306. Changing Restriction Enzyme Sites with oligos - (reply: 2)
  307. random cleavage of plasmids - insertional mutagenesis (reply: 2)
  308. Smear in my restriction digest - (reply: 12)
  309. Abnormal Nanodrop Results for pTurbo EcoRI digest - (reply: 7)
  310. Double Digest using enzymes from diff companies - (reply: 4)
  311. How to clean up restriction enzyme digestion - (reply: 8)
  312. full digestion of plasmid to get only dNTPs - (reply: 3)
  313. messed up restriction sites - (reply: 3)
  314. Cloning Issues with Rest Digest/Ligation/Transformation - (reply: 2)
  315. dpn1 digest - (reply: 5)
  316. Dnase digestion and PCR - (reply: 4)
  317. Restriction site - efficiency of restriction (reply: 4)
  318. Digestion of a plasmid - (reply: 1)
  319. different digestion pattern for same clone - (reply: 4)
  320. T4 DNA Ligase & Restriction Enzyme Expression - I am looking to save money! (reply: 4)
  321. RNA appearance on 1.5% gel after DNase I digestion - (reply: 2)
  322. Troubleshooting Digest, ligation, transformation - (reply: 7)
  323. Ligation after SalI & XhoI Digestion - (reply: 3)
  324. design primer - when I design a primer is it necessary to include the restriction (reply: 1)
  325. CIP required for double digestion of plasmid? - (reply: 3)
  326. Chromatin Remodelling / restriction enzyme accessibility - (reply: 1)
  327. Viral Shuttle Plasmid Restriction digests - (reply: 1)
  328. Why is Cleavage of DNA using restrcition enzyme more suitable for genetic engine - !! confused confused confused !! (reply: 4)
  329. Sequential restriction digestion of plasmid - (reply: 58)
  330. restriction enzyme based on forward or reverse sequence - restriction enzyme based on forward or reverse sequence (reply: 2)
  331. Digest genomic (eukaryote) DNA before running PCR, is it necessary? - I do not get any band from PCR using genomic DNA and different primers (reply: 6)
  332. problem in double digestion - (reply: 2)
  333. How to detect incomplete enzyme digests - (reply: 3)
  334. double digestion not working - (reply: 4)
  335. After double digestion, one weird band on the very top! - (reply: 4)
  336. After Double Digestion, there is one band on the very top. What is it? - (reply: 2)
  337. Restriction enzyme digest - (reply: 19)