Protocol Online logo
Top : New Forum Archives (2009-): : Molecular Biology

Restriction Enzymes - Enzymes from different companies work together? (Dec/21/2009 )

Our lab does not have many restriction enzymes and i was wondering if an enzyme from Invitrogen and one from NEB can work using NEB Buffers?


Check the composition of the buffers, but many will work well together - note that different REs often have different buffers.


I have many RE from various source and usually don't have a problem with mix and matching. The only time it really was an issue, what I ended up doing is looking at the buffer composition and after the first digestion I added only the missing components from the buffer of the next digestion.
Make up a stock solution of 10 fold of the missing components and add it to the reaction buffer.
Good luck