Digestion Protocols - (Oct/08/2009 )
I'm having some problems finding out a protocol to digest skin (Stratum corneum, epidermis, dermis) with enzymes!
I've determined that papain (to digest dermis - colagen) and trypsin (to digest epidermis) era the correct enzymes to the procedure, but there is nothing in the literature..I' ve looked everywhere.
It depends on what you want to do downstream?
Culture the cells / Protein isolation / Nucleic acid isolation…