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BSA + DTT versus -DTT - (Jun/15/2011 )

Hi,

I've just ran a SDS-PAGE gel with diferent proteins diluted in a buffer containing BSA. In the samples were I added DTT the BSA band did not ran as far as the same sample without DTT. What is the explanation for that?

-protchem-

protchem on Wed Jun 15 13:53:46 2011 said:


Hi,

I've just ran a SDS-PAGE gel with diferent proteins diluted in a buffer containing BSA. In the samples were I added DTT the BSA band did not ran as far as the same sample without DTT. What is the explanation for that?

Hola , with reducer the molecule is missfolded and have more difficlty to cross the acrylamide matrix; without the folded protein is more structurated, more compact and run a bit fast. Wait for the opinion of more forers that if Im wrong they give you a more specific explanation. Good luck

-protolder-