PMSF - (Feb/20/2009 )
Whats the role of PMSF in the protein storage. What it does?????
PMSF (phenylmethanesulphonylfluoride or phenylmethylsulphonyl fluoride) is a serine protease inhibitor therefore it binds specifically to the active serine residue in a serine proteases avoiding the proteolysis of these kind of proteases over your specific protein in a crude extract. It is very useful in purification procedures, but i know that it has a low half-life so you have to take this into account.
it is also irreversible so the proteases that it kills stay killed. the pmsf will only need to be replenished if anything is added to (or anything grows in) the solution.