PMSF - (Feb/20/2009 )
Whats the role of PMSF in the protein storage. What it does?????
-samita-
PMSF (phenylmethanesulphonylfluoride or phenylmethylsulphonyl fluoride) is a serine protease inhibitor therefore it binds specifically to the active serine residue in a serine proteases avoiding the proteolysis of these kind of proteases over your specific protein in a crude extract. It is very useful in purification procedures, but i know that it has a low half-life so you have to take this into account.
-paramyosin-
it is also irreversible so the proteases that it kills stay killed. the pmsf will only need to be replenished if anything is added to (or anything grows in) the solution.
-mdfenko-