How much R^2 that can be accept for standard curve of Bradford's assay? - (Mar/03/2009 )

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?

-MT-TIKY-

Yup it is

ButI like BCA or Lowry more thn Bradford for protein estimation.

TC

MT-TIKY on Mar 3 2009, 03:30 PM said:

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?

-T C-

T C on Mar 3 2009, 05:23 PM said:

Yup it is

ButI like BCA or Lowry more thn Bradford for protein estimation.

TC

MT-TIKY on Mar 3 2009, 03:30 PM said:

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?

THANKS

-MT-TIKY-

T C on Mar 3 2009, 02:23 AM said:

Yup it is

ButI like BCA or Lowry more thn Bradford for protein estimation.

TC

MT-TIKY on Mar 3 2009, 03:30 PM said:

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?

lowry has a higher chemical competibility than bradford

-Nrelo-

MT-TIKY on Mar 3 2009, 03:00 AM said:

I try to set standard curve by using BSA. I got R^2 = 0.987, is it acceptable?

It depends: Does the line fit the data (i.e. would a curve fit better?)? What are your residual plots like - random, non-random? Do you have any points on your standard curve outside of the given sensitivities of the Bradford assay?

-bob1-