Can anyone recommend some immunoprecipitation kits? - (Oct/16/2014 )
I will try to immunoprecipitate (IP) some small peptide from the insect blood (haemolymph). I have no IP experience. Can anyone recommend some immunoprecipitation kits to me?
I find Invitrogen/Life's protein A and protein G Dynabeads to be pretty reliable and easy to use. Not exactly a kit, but the buffers you find in most kits are very simple to prepare (and much cheaper).
Usually all you need for a straightforward IP is:
1) A good solid phase (I recommend Dynabeads and a magnet over agarose/sepharose slurry with centrifugation)
2) A simple IP buffer (NaCl between 100-300mM, 50mM Tris, and 0.1-1% Triton or NP40, pH 7.4 is a good start - adjust for your system).
3) A clean and verified antibody
With those it's just a matter of optimizing your antibody amount and IP method.
All that said, I had some decent luck with the Pierce magnetic direct IP/CoIP kit. Overall though, I don't think it performs any better than a home-made IP system.