Constitutively Ubiquitination Mark? - (Apr/03/2010 )
Hi again, earlier i had asked a question about constitutive acetylation marks.
Now, i need to know if an ubiquitin mimic exists. I am do not know of the DUB for the particular protein I'm studying.
Any suggestions? Thanks.
Have you tried FK2? It's an Ab for mono- and poly-ubiquitinated conjugates.
Doda on Apr 4 2010, 07:43 PM said:
I thought it will only blot for any ubiquitin present on a protein. I am looking to see if there is a such a thing as a ubiquitin-mimic like a phosphomemitic. I know in some of my experiments i'll be using an acetylation mimic by substitutin lysine residues for glutamate residues to mimic acetylation.
To be honest, I don't know if there is (are) such mimicking replacement(s), as for phospho- and acetyl-mimetics. But I've read some articles in which they replaced Leu/Ile by Ala in order to inhibit the UIM. Besides, there is also one study that showed that Ser residues flanking ub-Lys enhaced its ubiquitination (http://www.pnas.org/content/103/13/4958.full).
I'm not quite sure if it's exactly what you're looking for, but I hope your experiment works fine.
Btw, if you find out such a residue substitution that leads to increased Ub, please, let me know.
Good luck there!