Biotin - BMCC - (Apr/26/2011 )
I am performing an acyl biotin exchange with Biotin-BMCC (Pierce). I use avidin-beads from Pierce to isolate the biotinylated proteins. To get the proteins to come off of the beads, I add Non reducing buffer and boil for 7 minutes before performing Western blot. My protein of interest is only detected in non-reducing dye (Western blotting) hence I cannot use reducing dye. Is there as way around this?
you could use a different primary antibody (one that will recognize the reduced protein).