Too bright blue for bradford assay - (Jul/16/2009 )
Hi. I tried to maesure protein conc. by Bradford assay.
I got satisfactory standard curve, but I found something wierd.
My sample with bradford reagent is too bright. Actually I found the mixture had highest absorbance at 625nm.
What's wrong with my sample? Actually I avoided using detergents.
Thanks in advance.
-yja97-
yja97 on Jul 16 2009, 10:20 AM said:
Hi. I tried to maesure protein conc. by Bradford assay.
I got satisfactory standard curve, but I found something wierd.
My sample with bradford reagent is too bright. Actually I found the mixture had highest absorbance at 625nm.
What's wrong with my sample? Actually I avoided using detergents.
Thanks in advance.
I got satisfactory standard curve, but I found something wierd.
My sample with bradford reagent is too bright. Actually I found the mixture had highest absorbance at 625nm.
What's wrong with my sample? Actually I avoided using detergents.
Thanks in advance.
Could it be too concentrated? Try diluting it 1 in 10 or more.
-fishdoc-
Thanks for reply, fishdoc.
But I don't think my protein has high conc. I'm expecting around 10 ug/ml.
-yja97-
What else are in the buffer? Detergent components, such as Triton X 100 can interfere the results.
-genehunter-