stacking gel - Tris pH 8.8 (Aug/10/2009 )
Hi,
I made a solution of 1.5M tris with a required pH of 8.8. Howver, when I was adjusting the pH, I added too much HCL initially initially and had to adjust it by adding some alkaline. Is that ok? Will it affect my western?
-SF_HK-
No problem 1.5M has not to be very precise
-Gerard-
SF_HK on Aug 10 2009, 01:48 AM said:
Hi,
I made a solution of 1.5M tris with a required pH of 8.8. Howver, when I was adjusting the pH, I added too much HCL initially initially and had to adjust it by adding some alkaline. Is that ok? Will it affect my western?
I made a solution of 1.5M tris with a required pH of 8.8. Howver, when I was adjusting the pH, I added too much HCL initially initially and had to adjust it by adding some alkaline. Is that ok? Will it affect my western?
If I were you, I would throw away what you have prepared and make a brand new reagent. Be safe than sorry later.
M and pH of solutions do matter for obtaining good resolution. Evne if you don't run into problems with your current protein, your may, in the future, when you use the compromised reagent, forgetting how it was made.
-cellcounter-
also, the separation gel buffer is 1.5M, Ph 8.8. the stacking gel buffer is 1M, pH 6.8 (the pH being the more important parameter, volume used can be adjusted for differing concentration).
-mdfenko-
Hey,
Just make sure that the pH is correct and the molarity doesn't change. It will be okay.
Best,
TC
SF_HK on Aug 10 2009, 03:18 PM said:
Hi,
I made a solution of 1.5M tris with a required pH of 8.8. Howver, when I was adjusting the pH, I added too much HCL initially initially and had to adjust it by adding some alkaline. Is that ok? Will it affect my western?
I made a solution of 1.5M tris with a required pH of 8.8. Howver, when I was adjusting the pH, I added too much HCL initially initially and had to adjust it by adding some alkaline. Is that ok? Will it affect my western?
-T C-